TITLE

DNA detection on transistor arrays following mutation-specific enzymatic amplification

AUTHOR(S)
Pouthas, F.; Gentil, C.; Côte, D.; Bockelmann, U.
PUB. DATE
March 2004
SOURCE
Applied Physics Letters;3/1/2004, Vol. 84 Issue 9, p1594
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An integrated array of silicon field-effect transistor structures is used for electronic detection of label-free DNA. Measurements of the dc current–voltage characteristics of the transistors gives us access to reproducible detection of single- and double-stranded DNA, locally adsorbed on the surface of the device. We combine this approach with allele-specific polymerase chain reaction, to test for the 35delG mutation, a frequent mutation related to prelingual nonsyndromic deafness. © 2004 American Institute of Physics.
ACCESSION #
12360936

 

Related Articles

  • Maternally inherited deafness associated with a T1095C mutation in the mDNA. Tessa, Alessandro; Giannotti, Aldo; Tieri, Luigi; Vilarinho, Laura; Marotta, Giacomo; Santorelli, Filippo M // European Journal of Human Genetics;Feb2001, Vol. 9 Issue 2, p147 

    Hearing loss is a relatively frequent defect in children with a genetic or predisposition basis in about 50% of cases. Mitochondrial DNA (mtDNA)-associated disorder often present with sensorineural hearing loss (SNHL) either in isolation or as a part of a multisystem disorder in adults but the...

  • Contribution of GJB2 Mutations to Hearing Loss in the Hazara Division of Pakistan. Bukhari, Ihtisham; Mujtaba, Ghulam; Naz, Sadaf // Biochemical Genetics;Aug2013, Vol. 51 Issue 7/8, p524 

    Mutations of GJB2, which encodes connexin 26, are the most common cause of hereditary hearing loss in many human populations. This study was initiated to determine the prevalence of GJB2 mutations in individuals with hearing loss from the Hazara Division in Pakistan. We recruited 70 participants...

  • Mutations in the KCNQ4 gene are responsible for autosomal dominant deafness in four DFNA2 families. Coucke, Paul J.; Hauwe, Peter Van; Kelley, Philip M.; Kunst, Henricus; Schatteman, Isabelle; Van Velzen, Desiree; Meyers, Johan; Ensink, Robbert J.; Verstreken, Margriet; Declau, Frank; Marres, Henri; Kastury, Kumar; Bhasin, Shalender; McGuirt, Wyman T.; Smith, Richard J.H.; Cremers, Cor W.R.J.; Van de Heyning, Paul; Willems, Patrick J.; Smith, Shelley D.; Van Camp, Guy // Human Molecular Genetics;Jul99, Vol. 8 Issue 7, p1321 

    Examines mutations responsible for autosomal dominant deafness in families. Mapping of the gene; Missense mutations that alter conserved amino acids; Chromosomal localization of the gene.

  • Mutations in a novel cochlear gene cause DFNA9, a human nonsyndromic deafness with vestibular dysfunction. Robertson, Nahid G.; Lu, Leonard; Heller, Stefan; Merchant, Saumil N.; Eavey, Roland D.; McKenna, Michael; Nadol, Joseph B.; Miyamoto, Richard T.; Linthicum, Frederick H.; Lubianca Neto, José F.; Hudspeth, A.J.; Seidman, Christine E.; Morton, Cynthia C.; Seidman, J.G. // Nature Genetics;Nov98, Vol. 20 Issue 3, p299 

    DFNA9 is an autosomal dominant, nonsyndromic, progressive sensorineural hearing loss with vestibular pathology. Here we report three missense mutations in human COCH (previously described as Coch5b2), a novel cochlear gene, in three unrelated kindreds with DFNA9. All three residues mutated in...

  • Letter. Anichkina, Anna; Kulenich, Tatiana; Zinchenko, Sergey; Shagina, Irena; Polyakov, Aleksander; Ginter, Evgenii; Evgrafov, Oleg; Viktorova, Tatiana; Khusnitdonova, Elza // European Journal of Human Genetics;Feb2001, Vol. 9 Issue 2, p151 

    Investigates the association of 35delG allele mutation with deafness in Europe. Frequency of mutation; DNA analysis; Number of chromosomes with 35delG mutation.

  • Whole genome and transcriptome amplification: practicable tools for sustainable tissue biobanking? Teichman, Adriana; Storz, Martina; Dettwiler, Susanne; Moch, Holger; Schraml, Peter // Virchows Archiv;Nov2012, Vol. 461 Issue 5, p571 

    The use of whole genome amplification (WGA) and whole transcriptome amplification (WTA) techniques enables the enrichment of DNA and RNA from very small amounts of tissue. Here, we tested the suitability of WGA and WTA for tumor tissue biobanking. DNA and RNA from 13 standardized and seven...

  • Mutations in the LMNA gene do not cause axonal CMT in Czech patients. Laššuthová, Petra; Baránková, Lucia; Haberlová, Jana; Mazanec, Radim; Wallace, Andrew; Huehne, Kathrin; Rautenstrauss, Bernd; Seeman, Pavel // Journal of Human Genetics;Jun2009, Vol. 54 Issue 6, p365 

    The LMNA gene was sequenced in 98 Czech patients from 94 unrelated families with early-onset axonal Charcot–Marie–Tooth (CMT) disease consistent with both autosomal recessive inheritance and sporadic cases. Biallelic pathogenic mutations were not found in any patient in this group....

  • Detection of HIV cDNA Point Mutations with Rolling-Circle Amplification Arrays. Lingwei Wu; Quanjun Liu; Zhongwei Wu; Zuhong Lu // Molecules;Feb2010, Vol. 15 Issue 2, p619 

    In this paper we describe an isothermal rolling-circle amplification (RCA) protocol to detect gene point mutations on chips. The method is based on an allele-specific oligonucleotide circularization mediated by a special DNA ligase. The probe is circularized when perfect complementary sequences...

  • Hemoglobin Lansing (Alpha) [HBA2 CD87 (HIS>GLU) (C>A)] in a Turkish Individual Resulting from Another Nucleotide Substitution. Akar, Nejat; Torun, Didem; Öztürk, Ayşenur // Turkish Journal of Hematology;2014, Vol. 31 Issue 3, p317 

    The article presents a case study of a 21-year-old Turkish woman who was diagnosed with novel missense mutation found in codon 87 (DNA sequence). Topics discussed include isolation of DNA from a peripheral blood sample with the phenol-chloroform protocol, analysis of beta globin, hemoglobin,...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics