UVB Irradiation Alters Cellular Responses to Cytokines: Role in Extracellular Matrix Gene Expression

Werth, Victoria P.; Williams, Kevin Jon; Fisher, Edward A.; Bashir, Muhammad; Rosenbloom, Joel; Shi, Xiaomei
March 1997
Journal of Investigative Dermatology;Mar1997, Vol. 108 Issue 3, p290
Academic Journal
Solar radiation causes cutaneous photodamage characterized by alterations in the quantity and structure of the extracellular matrix. We determined the direct and cytokine-mediated effects of UV irradiation on mRNA levels for two matrix elements, tropoelastin and fibrillin 1. (i) Comparison of normal versus endstage photodamaged skin failed to reveal differences in these message levels. (ii) Acutely irradiated skin showed suppression of both tropoelastin and fibrillin mRNAs. (iii) UVB irradiation (50 mJ) of cultured skin fibroblasts suppressed fibrillin mRNA by 50%, consistent with a direct effect of radiation. Addition to the cultured fibroblasts of several cytokines upregulated by UVB showed that IL-1α had no effect on fibrillin mRNA in unirradiated cells, but in irradiated cells, this cytokine enhanced the suppression of fibrillin mRNA. There were no changes in the message stability, suggesting altered gene transcription. In contrast, UVB had no effect on tropoelastin mRNA levels in cultured fibroblasts, indicating the absence of a direct effect of radiation. IL-1α stimulated tropoelastin mRNA 2.8-fold in unirradiated cells, and this stimulation was entirely blocked by UVB. Overall, our results indicate acute suppression of matrix genes by UVB in vivo. The suppression of fibrillin message was a direct effect of UVB on fibroblasts and was augmented by IL-lα. Suppression of tropoelastin message by UVB occurred in vitro only in IL-1αstimulated cells. We conclude that UVB substantially alters the pattern of cellular response to cytokines. The interplay between UVB and cytokines is essential to explain the acute responses of matrix genes to UVB in vivo.


Related Articles

  • Control of Fibrosis in Systemic Scleroderma. Mauch, Cornelia; Eckes, Beate; Hunzelmann, Nicolas; Oono, Takashi; Kozlowska, Ewa; Krieg, Thomas // Journal of Investigative Dermatology;Jan1993, Vol. 100 Issue 1, p92S 

    Scleroderma is characterized by an excessive deposition of collagen in all involved organs. This is due to an overproduction of extracellular matrix (ECM) molecules following induction of gene expression, whereas there is no evidence that the composition of the connective tissue matrix is...

  • Jabra Expands Hands-Free Offerings.  // TWICE: This Week in Consumer Electronics;11/06/2000, Vol. 15 Issue 25, p39 

    Introduces EarSet, EarBoom and EarBud, hands-free communication products produced by Jabra company in San Diego, California. Integration of ergonomic ear mold EarGel on the products; Features and price of the products; Target consumers.

  • Hypersensitive site 7 of the TH2 locus control region is essential for expressing TH2 cytokine genes and for long-range intrachromosomal interactions. Lee, Gap Ryol; Spilianakis, Charalampos G.; Flavell, Richard A. // Nature Immunology;Jan2005, Vol. 6 Issue 1, p42 

    Several regulatory regions are important for the expression of genes encoding T helper type 2 (TH2) cytokines, including TH2-specific DNase I hypersensitivity sites in the TH2 cytokine locus control region. Among these sites, Rad50 hypersensitive site 7 (RHS7) shows rapid TH2-specific...

  • Suppressive Effect of Antioxidants on Intercellular Adhesion Molecule-1 (ICAM-1) Expression in Human Epidermal Keratinocytes. Ikeda, Mitsunori; Schroeder, Kendra K.; Mosher, Laura B.; Woods, Connie W.; Akeson, Ann L. // Journal of Investigative Dermatology;Dec94, Vol. 103 Issue 6, p791 

    Intercellular adhesion molecule-1 (ICAM-1) is strongly expressed by human epidermal keratinocytes during the course of inflammatory skin diseases. To test the possibility that reactive oxygen species produced in the skin during an inflammatory response affect ICAM-1 expression, cultured human...

  • Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3a Synthesis in Activated Macrophages. Jalonen, Ulla; Nieminen, Riina; Vuolteenaho, Katriina; Kankaanranta, Hannu; Moilanen, Eeva // Mediators of Inflammation;12/1/2006, Vol. 2006 Issue 6, p1 

    In inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We...

  • Infrared A Radiation Influences the Skin Fibroblast Transcriptome: Mechanisms and Consequences. Calles, Christian; Schneider, Maren; Macaluso, Filippina; Benesova, Tereza; Krutmann, Jean; Schroeder, Peter // Journal of Investigative Dermatology;Jun2010, Vol. 130 Issue 6, p1524 

    Infrared A (IRA) radiation (760–1440 nm) is a major component of solar radiation and, similar to UVR, causes photoaging of human skin by increasing the expression of matrix metalloproteinase-1 in human skin fibroblasts. In this study, we assessed the IRA-induced transcriptome in primary...

  • Expression of the Vanin Gene Family in Normal and Inflamed Human Skin: Induction by Proinflammatory Cytokines. Jansen, Patrick A. M.; Kamsteeg, Marijke; Rodijk-Olthuis, Diana; van Vlijmen-Willems, Ivonne M. J. J.; de Jongh, Gys J.; Bergers, Mieke; Tjabringa, Geuranne S.; Zeeuwen, Patrick L. J. M; Schalkwijk, Joost // Journal of Investigative Dermatology;Sep2009, Vol. 129 Issue 9, p2167 

    The vanin gene family encodes secreted and membrane-bound ectoenzymes that convert pantetheine into pantothenic acid and cysteamine. Recent studies in a mouse colitis model indicated that vanin-1 has proinflammatory activity and suggest that pantetheinases are potential therapeutic targets in...

  • Effector Cells. Vohr, Hans-Werner // Encyclopedic Reference of Immunotoxicology;2005, p221 

    A definition of the term "effector cells" is presented. It refers to short-lived cells involved in resolving the current antigenic challenge. They are commonly applied to activated CD4 T cells that secrete high levels of cytokines in response to antigen. An effector cell is used for a lymphocyte...

  • Tension-Compression Loading with Chemical Stimulation Results in Additive Increases to Functional Properties of Anatomic Meniscal Constructs. Huey, Daniel J.; Athanasiou, Kyriacos A. // PLoS ONE;2011, Vol. 6 Issue 11, p1 

    Objective This study aimed to improve the functional properties of anatomically-shaped meniscus constructs through simultaneous tension and compression mechanical stimulation in conjunction with chemical stimulation. Methods Scaffoldless meniscal constructs were subjected to simultaneous tension...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics