TITLE

UVB Irradiation Alters Cellular Responses to Cytokines: Role in Extracellular Matrix Gene Expression

AUTHOR(S)
Werth, Victoria P.; Williams, Kevin Jon; Fisher, Edward A.; Bashir, Muhammad; Rosenbloom, Joel; Shi, Xiaomei
PUB. DATE
March 1997
SOURCE
Journal of Investigative Dermatology;Mar1997, Vol. 108 Issue 3, p290
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Solar radiation causes cutaneous photodamage characterized by alterations in the quantity and structure of the extracellular matrix. We determined the direct and cytokine-mediated effects of UV irradiation on mRNA levels for two matrix elements, tropoelastin and fibrillin 1. (i) Comparison of normal versus endstage photodamaged skin failed to reveal differences in these message levels. (ii) Acutely irradiated skin showed suppression of both tropoelastin and fibrillin mRNAs. (iii) UVB irradiation (50 mJ) of cultured skin fibroblasts suppressed fibrillin mRNA by 50%, consistent with a direct effect of radiation. Addition to the cultured fibroblasts of several cytokines upregulated by UVB showed that IL-1α had no effect on fibrillin mRNA in unirradiated cells, but in irradiated cells, this cytokine enhanced the suppression of fibrillin mRNA. There were no changes in the message stability, suggesting altered gene transcription. In contrast, UVB had no effect on tropoelastin mRNA levels in cultured fibroblasts, indicating the absence of a direct effect of radiation. IL-1α stimulated tropoelastin mRNA 2.8-fold in unirradiated cells, and this stimulation was entirely blocked by UVB. Overall, our results indicate acute suppression of matrix genes by UVB in vivo. The suppression of fibrillin message was a direct effect of UVB on fibroblasts and was augmented by IL-lα. Suppression of tropoelastin message by UVB occurred in vitro only in IL-1αstimulated cells. We conclude that UVB substantially alters the pattern of cellular response to cytokines. The interplay between UVB and cytokines is essential to explain the acute responses of matrix genes to UVB in vivo.
ACCESSION #
12286462

 

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