TITLE

Effects of antiplatelet agents on pulmonary haemodynamic response to fMLP in endotoxin primed rats

AUTHOR(S)
Song C; Suzuki S; Kubo H; Chida M; Hoshikawa Y; Tabata T; Kondo T
PUB. DATE
January 2004
SOURCE
Thorax;Jan2004, Vol. 59 Issue 1, p39
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
BACKGROUND: The interaction between neutrophils and platelets may be important in the modulation of pulmonary haemodynamics under systemic inflammatory conditions. A study was undertaken to examine whether antiplatelet agents inhibit platelet-neutrophil adherence and ameliorate the pulmonary haemodynamic response to fMLP by inhibiting thromboxane release in endotoxin primed lungs. fMLP stimulates neutrophils but not platelets; however, thromboxane synthesis in neutrophils is very low. METHODS: Rats were pretreated with either cilostazol (300 mg/kg) or aspirin (50 mg/kg) before endotoxin priming (5 mg/kg). Platelets in the lung were identified by fluorescent immunohistochemistry. Platelet-neutrophil adherence was analysed by flow cytometry of the lung vascular flush. The effect of fMLP (10(-6) M) on thromboxane release, lung weight (an indicator of pulmonary vasoconstriction), and lung filtration coefficient was determined in an isolated lung system perfused at a constant pressure difference. RESULTS: Endotoxin induced platelet accumulation and platelet-neutrophil adherence in the lung capillary were completely inhibited by cilostazol and aspirin while neutrophil recruitment was not affected. The fMLP challenge caused a significant increase in thromboxane B2 levels in endotoxin primed lungs. The fMLP induced decrease in lung weight was enhanced by endotoxin priming (from -4.9 to -63.9 mg, 95% CI of mean difference -99.5 to -10.5 mg, p<0.05). The fMLP induced increase in the lung filtration coefficient was also enhanced by endotoxin priming (from 0.63 to 2.40 mg/min/cm H2O/g, 95% CI of mean difference 1.17 to 2.37 mg/min/cm H2O/g, p<0.05). Treatment with cilostazol and aspirin completely inhibited the enhanced pulmonary haemodynamic response to fMLP. CONCLUSION: The neutrophil-platelet interaction is of critical importance in the modulation of pulmonary haemodynamics via thromboxane.
ACCESSION #
12137039

 

Related Articles

  • Roles for thromboxane A2 and leukotrienes in endotoxin-induced acute renal failure. Badr, Kamal F.; Kelley, Vicki E.; Rennke, Helmut G.; Brenner, Barry M. // Kidney International;Oct1986, Vol. 30 Issue 4, p474 

    Bolus i.v. administration of 100 μg/kg of E. Coli lipopolysaccharide endotoxin (LPS) to adult male Munich-Wistar rats (N = 18) resulted in a progressive fall in RBF and GFR from 6.9 ± 0.2 SE and 1.1 ± 0.05 ml/min to minimal values at 50 minutes of 3.8 ± 0.4 and 0.32 ± 0.08 (P <...

  • Ozone-Induced Mucous Cell Metaplasia. Luster, Michael I. // Toxicological Sciences;Apr2001, Vol. 60 Issue 2, p193 

    The article highlighted in this issue is “Endotoxin Enhancement of Ozone-Induced Mucous Cell Metaplasia Is Neutrophil-Dependent in Rat Nasal Epithelium,” by James G. Wagner, Steven J. Van Dyken, Jon A. Hotchkiss, and Jack R. Harkema (pp. 338–347).

  • Effects of Oral Administration of Antioxidant Taurine on Haemotological Parameters in Wistar Rats. Anand, P.; Rajakumar, D.; Felix, A. John William; Balasubramanian, T. // Pakistan Journal of Biological Sciences;2010, Vol. 13 Issue 16, p785 

    No abstract available.

  • The effects of PPAR-? ligand pioglitazone on platelet aggregation and arterial thrombus formation. Li, Dayuan; Chen, Kui; Sinha, Nandita; Zhang, Xingjiang; Wang, Yin; Sinha, Anjan K.; Romeo, Francesco; Mehta, Jawahar L. // Cardiovascular Research;Mar2005, Vol. 65 Issue 4, p907 

    Abstract: Background: It has been suggested that peroxisome proliferator-activated receptor (PPAR)-? ligands reduce the development of atherosclerosis and myocardial ischemia�reperfusion injury; both of these phenomena are associated with platelet activation. We postulated that PPAR-?...

  • Hypertonic saline reduces inflammation and enhances the resolution of oleic acid induced acute lung injury. Kennedy, Muiris T.; Higgins, Brendan D.; Costello, Joseph F.; Curtin, William A.; Laffey, John G. // BMC Pulmonary Medicine;2008, Vol. 8, Special section p1 

    Background: Hypertonic saline (HTS) reduces the severity of lung injury in ischemia-reperfusion, endotoxin-induced and ventilation-induced lung injury. However, the potential for HTS to modulate the resolution of lung injury is not known. We investigated the potential for hypertonic saline to...

  • Anti-Inflammatory Effects of Lipoxins on Lipopolysaccharide-Induced Uveitis in Rats. Karim, Md. Jahurul; Bhattacherjee, Parimal; Biswas, Sumana; Paterson, Christopher A. // Journal of Ocular Pharmacology & Therapeutics;Dec2009, Vol. 25 Issue 6, p483 

    Purpose: Lipoxins exert potent anti-inflammatory and pro-resolving actions by reducing polymorphonuclear neutrophil (PMN) infiltration. This study describes the effect of lipoxin A4 and a stable analog on the resolution of ocular inflammation induced by intravitreal injection of...

  • Platelet-Mediated Cardioprotective Effect Against Ischemia-Reperfusion Injury in Isolated Rat Hearts: Role of Platelet Number and Contribution of Supernatant of Aggregated Platelets. Baichun Yang; Mehta, Paulette; Mehta, Jaawhar L. // Journal of Cardiovascular Pharmacology & Therapeutics;Mar1998, Vol. 3 Issue 1, p23 

    The article focuses on the role of platelet number and the contribution of the supernatant of aggregated platelets in cardioprotection. Rats were subjected to ischemia and reperfusion which induced myocardial dysfunction. Results show that rat hearts perfused with platelets and supernatant of...

  • Pravastatin and Clopidogrel Combined Inhibit Intimal Hyperplasia in a Rat Carotid Endarterectomy Model. Bledsoe, Shelly L.; Barr, John C.; Fitzgerald, Ryan T.; Brown, Aliza T.; Faas, Fred H.; Eidt, John F.; Moursi, Mohammed M. // Vascular & Endovascular Surgery;Jan/Feb2006, Vol. 40 Issue 1, p49 

    Intimal hyperplasia, resulting from a complex cascade of events involving platelets, leukocytes, and smooth muscle cells, may be inhibited by the HMG-CoA reductase inhibitor pravastatin, which demonstrates inhibition of platelet activity and leukocyte adhesion and may be associated with...

  • The Effects of Short-Term Training on Platelet Functions and Total Antioxidant Capacity in Rats. Ficicilar, H.; Zergeroglu, A. M.; Ersoz, G.; Erdogan, A.; Ozdemir, S.; Tekin, D. // Physiological Research;2006, Vol. 55 Issue 2, p151 

    The purpose of the present study was to investigate the effect of short-term endurance training on plasma total antioxidant status (TAS) and on in vitro platelet aggregation and ATP release. Blood samples were collected from the abdominal aorta of rats following short-term treadmill exercise (25...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics