Sequence Conservation, HLA-E-Restricted Peptide, and Best-Defined CTL/CD8+ Epitopes in Gag P24 (Capsid) of HIV-1 Subtype B

Prasetyo, Afiono Agung; Dharmawan, Ruben; Sari, Yulia; Sariyatun, Ratna
February 2017
AIP Conference Proceedings;2017, Vol. 1813 Issue 1, p1
Conference Proceeding
Human immunodeficiency virus type 1 (HIV-1) remains a cause of global health problem. Continuous studies of HIV-1 genetic and immunological profiles are important to find strategies against the virus. This study aimed to conduct analysis of sequence conservation, HLA-E-restricted peptide, and best-defined CTL/CD8+ epitopes in p24 (capsid) of HIV-1 subtype B worldwide. The p24-coding sequences from 3,557 HIV subtype B isolates were aligned using MUSCLE and analysed. Some highly conserved regions (sequence conservation =95%) were observed. Two considerably long series of sequences with conservation of 100% was observed at base 349-356 and 550-557 of p24 (HXB2 numbering). The consensus from all aligned isolates was precisely the same as consensus B in the Los Alamos HIV Database. The HLA-E-restricted peptide in amino acid (aa) 14-22 of HIV-1 p24 (AISPRTLNA) was found in 55.9% (1,987/3,557) of HIV-1 subtype B worldwide. Forty-four best-defined CTL/CD8+ epitopes were observed, in which VKNWMTETL epitope (aa 181-189 of p24) restricted by B*4801 was the most frequent, as found in 94.9% of isolates. The results of this study would contribute information about HIV-1 subtype B and benefits for further works willing to develop diagnostic and therapeutic strategies against the virus.


Related Articles

  • Nonnucleoside Reverse Transcriptase Inhibitor-Sparing Antiretroviral Regimens for Treatment-Naive Volunteers Infected With HIV-1.  // Annals of Internal Medicine;10/7/2014, Vol. 161 Issue 7, pI-22 

    The article presents questions and answers related to HIV-1 treatment including how was study done, researchers findings and limitations of the study.

  • Optimal Diagnostic Tests in Primary HIV-1 Infection. Sadovsky, Richard // American Family Physician;9/15/2001, Vol. 64 Issue 6, p1091 

    Presents an abstract of the study `Diagnosis of Primary HIV-1 Infection,' by E.S Daar et al., from the January 2, 2001 issue of the `Annals of Internal Medicine.'

  • Clinical Implications of Identifying Non-B Subtypes of Human Immunodeficiency Virus Type 1... Walter, Elizabeth A.; Gilliam, Bruce; Delmar, Judy A.; Spooner, Katherine; Morris, Joseph T.; Aronson, Naomi; Wegner, Scott A.; Michael, Nelson L.; Jagodzinski, Linda L. // Clinical Infectious Diseases;9/1/2000, Vol. 31 Issue 3, p798 

    Presents the clinical implications of identifying non-B subtypes of HIV-type 1 infection. Identification of non-B subtypes of HIV-type 1 based on the low or undetected viral load; Heterogeneity among HIV-type 1 isolates; Three major phylogenetic groups of HIV; Prevalence and geographic...

  • Modeling secondary level of HIV contact tracing: its impact on HIV intervention in Cuba. Ying-Hen Hsieh; Yun-Shih Wang; de Arazoza, Hector; Lounes, Rachid // BMC Infectious Diseases;2010, Vol. 10 Issue 1, p194 

    Background: Universal HIV testing/treatment program has currently been suggested and debated as a useful strategy for elimination of HIV epidemic in Africa, although not without practical issues regarding the costs and feasibility of a fully implemented program. Methods: A mathematical model is...

  • Comment on "Using geospatial mapping to design HIV elimination strategies for sub-Saharan Africa". Fontaine, Christopher; Mahy, Mary; Izazola, José-Antonio; Ghys, Peter D. // Science Translational Medicine;8/2/2017, Vol. 9 Issue 401, p1 

    No abstract available.

  • Tests for infection with HIV: slandered goods. Mortimer, Philip P. // British Medical Journal (Clinical Research Edition);6/11/1988, Vol. 296 Issue 6637, p1615 

    Examines the accuracy of serological diagnosis for HIV infection in Africa. Availability of the tests; Characterization of courses for HIV infection;Development of antigenemia during the time between HIV exposure and antibody response.

  • HIV dectection on dried blood spot specimens in neonates.  // American Family Physician;10/1/1996, Vol. 54 Issue 5, p1743 

    Presents a summary of the article `Early detection of human immunodeficiency virus on dried blood spot specimens: Sensitivity across serial spcimens,' by A.M. Comeau, and others, from the Journal of Pediatrics, dated 1996.

  • The HIV epidemic that isn't. Verburg, Peter // Alberta Report / Newsmagazine;2/6/95, Vol. 22 Issue 8, p33 

    Reports that only a relatively small number of heterosexuals tested positive for the human immunodeficiency virus (HIV) in a 1994 test of 55,000 Albertans, according to a report released two weeks ago by the Provincial AIDS Program, a division of Alberta Health. Groups that tested positive for...

  • Gay Men's Health Crisis initiates HIV testing campaign.  // New York Amsterdam News;06/17/99, Vol. 90 Issue 25, p14 

    Reports on a HIV testing campaign in New York City. Disproportionate threat of HIV to blacks; Contact information.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics