TITLE

Evaluation of a Rapid Quantitative Diagnostic Test for Adenovirus Type 4

AUTHOR(S)
Faix, Dennis J.; Houng, Huo-Shu H.; Gaydos, Joel C.; Liu, Shih-Kang S.; Connors, John T.; Brown, Xiomara; Asher, Ludmila V.; Vaughn, David W.; Binn, Leonard N.
PUB. DATE
February 2004
SOURCE
Clinical Infectious Diseases;2/1/2004, Vol. 38 Issue 3, p391
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Acute respiratory disease (ARD) due to adenoviruses is a reemerging disease in military recruits. It is a challenge for clinicians to accurately diagnose this disease and to appropriately treat affected individuals. This study investigated the utility of a quantitative, rapid-cycle, real-time fluorogenic polymerase chain reaction (PCR) technique for detecting adenovirus type 4 (Ad4) in a clinical setting. Throat swab specimens and clinical data were collected from US Army basic trainees hospitalized with ARD at Fort Jackson, South Carolina. A total of 140 throat swab specimens were collected from 83 subjects. Rapid PCR results (obtained in <2 h) had a sensitivity of 100% and a specificity of 100%, compared with viral culture. There was no difference, qualitative or quantitative, between frozen and fresh samples for PCR detection of Ad4. Individuals with test results positive for Ad4 were hospitalized longer than were individuals with negative test results. Higher virus loads at hospital admission corresponded to longer lengths of stay for Ad4-positive subjects.
ACCESSION #
12080866

 

Related Articles

  • Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Lu, X.; Erdman, D. D. // Archives of Virology;Aug2006, Vol. 151 Issue 8, p1587 

    Human adenoviruses (Ads) are responsible for a substantial disease burden. Type-specific identification of Ads can help guide therapeutic and disease prevention strategies and aid epidemiological investigations. Immunotyping of Ads by serum neutralization (SN) is laborious and time consuming and...

  • A new example of viral intein in Mimivirus. Ogata, Hiroyuki; Raoult, Didier; Claverie, Jean-Michel // Virology Journal;2005, Vol. 2, p8 

    Background: Inteins are "protein introns" that remove themselves from their host proteins through an autocatalytic protein-splicing. After their discovery, inteins have been quickly identified in all domains of life, but only once to date in the genome of a eukaryote-infecting virus. Results:...

  • A cost effective real-time PCR for the detection of adenovirus from viral swabs. Al-Siyabi, Turkiya; Binkhamis, Khalifa; Wilcox, Melanie; Wong, Sallene; Pabbaraju, Kanti; Tellier, Raymond; Hatchette, Todd F.; LeBlanc, Jason J. // Virology Journal;2013, Vol. 10 Issue 1, p1 

    Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost...

  • Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control. Alsaleh, Asma N.; Whiley, David M.; Bialasiewicz, Seweryn; Lambert, Stephen B.; Ware, Robert S.; Nissen, Michael D.; Sloots, Theo P.; Grimwood, Keith // BMC Infectious Diseases;2014, Vol. 14 Issue 1, p1 

    Background Carefully conducted, community-based, longitudinal studies are required to gain further understanding of the nature and timing of respiratory viruses causing infections in the population. However, such studies pose unique challenges for field specimen collection, including as we have...

  • From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area. Leifels, Mats; Hamza, Ibrahim Ahmed; Krieger, Marion; Wilhelm, Michael; Mackowiak, Martin; Jurzik, Lars // PLoS ONE;11/23/2016, Vol. 11 Issue 11, p1 

    Quantitative PCR methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. However, conclusions for public health drawn from results of such molecular techniques are limited due to...

  • Development of a nested PCR assay to detect equine infectious anemia proviral DNA from peripheral blood of naturally infected horses. Dong, Jian-Bao; Zhu, Wei; Cook, Frank; Goto, Yoshitaka; Horii, Yoichiro; Haga, Takeshi // Archives of Virology;Nov2012, Vol. 157 Issue 11, p2105 

    Equine infectious anemia (EIA) has posed a major challenge and caused significant losses to the equine industry worldwide. PCR detection methods have considerable potential as an adjunct to conventional serological diagnostic techniques. However, most published PCR methods, including that...

  • Discovery of a novel circular DNA virus in the Forbes sea star, Asterias forbesi. Fahsbender, Elizabeth; Hewson, Ian; Rosario, Karyna; Tuttle, Allison; Varsani, Arvind; Breitbart, Mya // Archives of Virology;Sep2015, Vol. 160 Issue 9, p2349 

    A single-stranded DNA (ssDNA) virus, Asterias forbesi-associated circular virus (AfaCV), was discovered in a Forbes sea star displaying symptoms of sea star wasting disease (SSWD). The AfaCV genome organization is typical of circular Rep-encoding ssDNA (CRESS-DNA) viruses and is similar to that...

  • Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes. Li, Huixia; Zhu, Xueliang; Zheng, Yadong; Wang, Shuai; Liu, Zhenyong; Dou, Yongxi; Li, Hui; Cai, Xuepeng; Luo, Xuenong // Archives of Virology;Jul2013, Vol. 158 Issue 7, p1477 

    Orf virus (ORFV) is an enveloped virus with a double-stranded DNA genome, causing a contagious pustular dermatitis, mainly in goats and sheep. In this study, two strains of ORFV were isolated from sheep and goat samples in Xinjiang and Shaanxi, China. The B2L and virus interferon resistance...

  • Frequency and subtype of BK virus infection in Iranian patients infected with HIV. Akhgari, Shahla; Mohraz, Minoo; Azadmanesh, Kayhan; Vahabpour, Rouhollah; Kazemimanesh, Monireh; Aghakhani, Arezoo; Jozpanahi, Manizheh; Banifazl, Mohammad; Bavand, Anahita; Ramezani, Amitis // Medical Microbiology & Immunology;Feb2016, Vol. 205 Issue 1, p57 

    Human polyomavirus BK virus (BKV) is a double-stranded DNA virus that infects approximately 90 % of the general population as a subclinical or mild infection. In immunosuppressed patients, such as HIV cases, BKV may be reactivated resulting hemorrhagic cystitis and tubulointerstitial nephritis....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics