TITLE

Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy

AUTHOR(S)
Tardif, Pier-Luc; Bertrand, Marie-Jeanne; Abran, Maxime; Castonguay, Alexandre; Lefebvre, Joël; Stähli, Barbara E.; Merlet, Nolwenn; Mihalache-Avram, Teodora; Geoffroy, Pascale; Mecteau, Mélanie; Busseuil, David; Feng Ni; Abulrob, Abedelnasser; Rhéaume, Éric; L'Allier, Philippe; Tardif, Jean-Claude; Lesage, Frédéric
PUB. DATE
December 2016
SOURCE
International Journal of Molecular Sciences;Dec2016, Vol. 17 Issue 12, p2110
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology.
ACCESSION #
120516934

 

Related Articles

  • Clogged Arteries? Not All Arterial Plaque is Equally Dangerous.  // Women's Health Advisor;Jul2014, Vol. 18 Issue 7, p4 

    The article focuses on the arterial plaque which causes heart attack. Topics discussed include the comparison between types of arterial plaques which are stable and unstable plaque according to the cardiologist Dr. Robert J. Kim, MD, the several cardiac risk factors which can cause plaque such...

  • Combined optical coherence tomography and intravascular ultrasound radio frequency data analysis for plaque characterization. Classification accuracy of human coronary plaques in vitro. Goderie, T.; Soest, G.; Garcia-Garcia, H.; Gonzalo, N.; Koljenović, S.; Leenders, G.; Mastik, F.; Regar, E.; Oosterhuis, J.; Serruys, P.; Steen, A. // International Journal of Cardiovascular Imaging;Dec2010, Vol. 26 Issue 8, p843 

    This study was performed to characterize coronary plaque types by optical coherence tomography (OCT) and intravascular ultrasound (IVUS) radiofrequency (RF) data analysis, and to investigate the possibility of error reduction by combining these techniques. Intracoronary imaging methods have...

  • Intracoronary near-infrared spectroscopy: an overview of the technology, histologic validation, and clinical applications. O'Brien, Andrew; LaCombe, Andrew; Stickland, Aubrey; Madder, Ryan D. // Global Cardiology Science & Practice;2016, Vol. 2016 Issue 2, p1 

    Intracoronary near-infrared spectroscopy (NIRS) imaging, which is now clinically available in a combined NIRS and intravascular ultrasound catheter, is a novel catheter-based imaging modality capable of identifying lipid core plaque within the coronary arteries of living patients. The present...

  • Relationship between cardiovascular risk factors and biomarkers with necrotic core and atheroma size: a serial intravascular ultrasound radiofrequency data analysis. García-García, Héctor; Klauss, Volker; Gonzalo, Nieves; Garg, Scot; Onuma, Yoshinobu; Hamm, Christian; Wijns, William; Shannon, Jennifer; Serruys, Patrick // International Journal of Cardiovascular Imaging;Apr2012, Vol. 28 Issue 4, p695 

    We explored the impact of patient demographics, anthropometric measurements, cardiovascular risk factors, and soluble biomarkers on necrotic core and atheroma size in patients with coronary disease. The IBIS-2 trial enrolled 330 patients. In the multivariate analysis, at baseline, creatinine had...

  • Plaque Vulnerability as Assessed by Radiofrequency Intravascular Ultrasound in Patients with Valvular Calcification. Senguttuvan, Nagendra Boopathy; Kumar, Sharath; Lee, Wang-Soo; Mishra, Sundeep; Cho, Jun Hwan; Kwon, Jee Eun; Hyeon, Seong Hyeop; Jeong, Yun Sang; Won, Hoyoun; Shin, Seung Yong; Lee, Kwang Je; Kim, Tae Ho; Kim, Chee Jeong; Kim, Sang-Wook // PLoS ONE;11/2/2016, Vol. 11 Issue 11, p1 

    Background: Cardiac valvular calcification is associated with the overall coronary plaque burden and considered an independent cardiovascular risk and prognostic factor. The purpose of this study was to evaluate the relationship between the presence of valvular calcification and plaque...

  • palpography.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1690 

    A definition of the term "palpography" which refers to imaging of atherosclerotic plaques with intravascular ultrasonography is presented.

  • Optical coherence tomography: has its time come? Lindsay, Alistair C.; Viceconte, Nicola; Di Mario, Carlo // Heart;Sep2011, Vol. 97 Issue 17, p1361 

    The authors reflect on the optical coherence tomography (OCT). The authors mentions the use of OCT in the examination of stent trut in patients. The authors state that in 5,931 patients examined with stent strut, 98.4% showed adequate neointimal coverage and revealed the absence of intra-stent...

  • Very late bare metal stent thrombosis due to neoatherosclerotic plaque rupture: an optical coherence tomography finding. Bennett, Johan; Coosemans, Mark; Adriaenssens, Tom // Heart;Oct2012, Vol. 98 Issue 19, p1470 

    The article reports the finding of Very Late Stent Thrombosis (VLST) by optical coherence tomography caused by neoatherosclerotic plaque rupture.

  • OCT identified nonobstructive STEMI lesions that do not need stenting.  // Cardiology Today;Apr2013, Vol. 16 Issue 4, p24 

    The article discusses research on optical coherence tomography identification of non-obstructive plaque erosion lesions that do not need stenting, referencing the study by F. Prati that was published in the 2013 volume 6 issue of "Journal of American College of Cardiology: Cardiovascular Imaging."

  • Cappela begins Sideguard 3 trial; CleveMed gets CE mark, CMDCAS.  // Medical Device Daily;11/20/2009, Vol. 13 Issue 225, p1 

    The article reports that Cappella has started Sideguard 3, an intravascular ultrasound/optical coherence tomography (IVUS/OCT) evaluation of the Sideguard coronary side-branch stent and delivery system. Galway is a medical device company, based in Galway, Ireland, which develops solutions for...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics