TITLE

Differential Cytotoxic Potential of Silver Nanoparticles in Human Ovarian Cancer Cells and Ovarian Cancer Stem Cells

AUTHOR(S)
Yun-Jung Choi; Jung-Hyun Park; Jae Woong Han; Eunsu Kim; Oh Jae-Wook; Seung Yoon Lee; Jin-Hoi Kim; Gurunathan, Sangiliyandi
PUB. DATE
December 2016
SOURCE
International Journal of Molecular Sciences;Dec2016, Vol. 17 Issue 12, p2077
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The cancer stem cell (CSC) hypothesis postulates that cancer cells are composed of hierarchically-organized subpopulations of cells with distinct phenotypes and tumorigenic capacities. As a result, CSCs have been suggested as a source of disease recurrence. Recently, silver nanoparticles (AgNPs) have been used as antimicrobial, disinfectant, and antitumor agents. However, there is no study reporting the effects of AgNPs on ovarian cancer stem cells (OvCSCs). In this study, we investigated the cytotoxic effects of AgNPs and their mechanism of causing cell death in A2780 (human ovarian cancer cells) and OvCSCs derived from A2780. In order to examine these effects, OvCSCs were isolated and characterized using positive CSC markers including aldehyde dehydrogenase (ALDH) and CD133 by fluorescence-activated cell sorting (FACS). The anticancer properties of the AgNPs were evaluated by assessing cell viability, leakage of lactate dehydrogenase (LDH), reactive oxygen species (ROS), and mitochondrial membrane potential (mt-MP). The inhibitory effect of AgNPs on the growth of ovarian cancer cells and OvCSCs was evaluated using a clonogenic assay. Following 1-2 weeks of incubation with the AgNPs, the numbers of A2780 (bulk cells) and ALDH+/CD133+ colonies were significantly reduced. The expression of apoptotic and anti-apoptotic genes was measured by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Our observations showed that treatment with AgNPs resulted in severe cytotoxicity in both ovarian cancer cells and OvCSCs. In particular, AgNPs showed significant cytotoxic potential in ALDH+/CD133+ subpopulations of cells compared with other subpopulation of cells and also human ovarian cancer cells (bulk cells). These findings suggest that AgNPs can be utilized in the development of novel nanotherapeutic molecules for the treatment of ovarian cancers by specific targeting of the ALDH+/CD133+ subpopulation of cells.
ACCESSION #
120516901

 

Related Articles

  • Bcl-2 family proteins. Reed, John C // Oncogene;12/24/98 Review, Vol. 17 Issue 25, p3225 

    Bcl-2 family proteins serve as critical regulators of pathways involved in apoptosis, acting to either inhibit or promote cell death. Altered expression of these proteins occurs commonly in human cancers, contributing to neoplastic cell expansion by suppressing programmed cell death and...

  • Oncolytic viral therapy: targeting cancer stem cells. Smith, Tyrel T.; Roth, Justin C.; Friedman, Gregory K.; Gillespie, G. Yancey // Oncolytic Virotherapy;2014, Vol. 3, p21 

    Cancer stem cells (CSCs) are defined as rare populations of tumor-initiating cancer cells that are capable of both self-renewal and differentiation. Extensive research is currently underway to develop therapeutics that target CSCs for cancer therapy, due to their critical role in tumorigenesis,...

  • Cancer stem cells and tumor metastasis. Shaoqiang Sun; Xue Shan Qiu // Journal of Cancer Research & Therapeutics;Nov2013 Supplement 3, Vol. 9 Issue S3, pS148 

    Cancer stem cells (CSCs) are endowed with an inherent resistance to cytotoxic drugs, and are closely related to the migration, invasiveness, and anti-apoptotic ability of the cancer cells. Epithelial-mesenchymal transition (EMT) is a process where epithelial cells acquire the highly invasive and...

  • Integrin α3 is overexpressed in glioma stem-like cells and promotes invasion. Nakada, M; Nambu, E; Furuyama, N; Yoshida, Y; Takino, T; Hayashi, Y; Sato, H; Sai, Y; Tsuji, T; Miyamoto, K-i; Hirao, A; Hamada, J-i // British Journal of Cancer;6/25/2013, Vol. 108 Issue 12, p2516 

    Background:Glioma stem-like cell (GSC) properties are responsible for gliomagenesis and recurrence. GSCs are invasive but its mechanism remains to be elucidated. Here, we attempted to identify the molecules that promote invasion in GSCs.Methods:Neurospheres and CD133+ cells were collected from...

  • A c-Met inhibitor increases the chemosensitivity of cancer stem cells to the irinotecan in gastric carcinoma. Yashiro, M; Nishii, T; Hasegawa, T; Matsuzaki, T; Morisaki, T; Fukuoka, T; Hirakawa, K // British Journal of Cancer;11/12/2013, Vol. 109 Issue 10, p2619 

    Background:Cancer stem cells (CSCs) may be postulated mediators of the chemoresistance. This study aimed to determine an effective signal inhibitor with effects on the proliferation of CSCs in combination with anticancer drugs.Methods:We used three gastric cancer cell lines and three side...

  • In this issue.  // Nature Reviews Drug Discovery;Jul2014, Vol. 13 Issue 7, p477 

    An introduction is presented in which the editor discusses various reports within the issue on topics including cancer stem cells, anticancer strategies, and pathogenesis of neuropathic pain.

  • Re: Role of Oncogenic K-Ras in Cancer Stem Cell Activation by Aberrant Wnt/β-Catenin Signaling. Cristóbal, Ion; Rincón, Raúl; Manso, Rebeca; Rojo, Federico; García-Foncillas, Jesús // JNCI: Journal of the National Cancer Institute;Aug2014, Vol. 106 Issue 8, p1 

    A letter to the editor is presented in response to the article "Role of Oncogenic K-Ras in Cancer Stem Cell Activation by Aberrant Wnt/β-Catenin Signaling," by BS Moon and colleagues in issue number 2014;106(2).

  • Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny. Kiyonori Tanoue; Yuqing Wang; Minako Ikeda; Kaoru Mitsui; Rie Irie; Takao Setoguchi; Setsuro Komiya; Shoji Natsugoe; Ken-ichiro Kosai // Journal of Translational Medicine;2014, Vol. 12 Issue 1, p1 

    Background Effective methods for eradicating cancer stem cells (CSCs), which are highly tumorigenic and resistant to conventional therapies, are urgently needed. Our previous studies demonstrated that survivin-responsive conditionally replicating adenoviruses regulated with multiple factors...

  • Rac1 is required for oncolytic NDV replication in human cancer cells and establishes a link between tumorigenesis and sensitivity to oncolytic virus. Puhlmann, J.; Puehler, F.; Mumberg, D.; Boukamp, P.; Beier, R. // Oncogene;4/15/2010, Vol. 29 Issue 15, p2205 

    Oncolytic Newcastle disease virus (NDV) replicates selectively in most human tumor cells but not in normal cells. The relationship between tumorigenesis and the selective susceptibility of most tumor cells to oncolytic NDV replication is poorly understood. A multistage skin carcinogenesis model...

  • Overcoming Barriers in Oncolytic Virotherapy with HDAC Inhibitors and Immune Checkpoint Blockade. Marchini, Antonio; Scott, Eleanor M.; Rommelaere, Jean // Viruses (1999-4915);Jan2016, Vol. 8 Issue 1, p9 

    Oncolytic viruses (OVs) target and destroy cancer cells while sparing their normal counterparts. These viruses have been evaluated in numerous studies at both pre-clinical and clinical levels and the recent Food and Drug Administration (FDA) approval of an oncolytic herpesvirus-based treatment...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics