Comparative Evaluation of TRAIL, FGF-2 and VEGF-A-Induced Angiogenesis In Vitro and In Vivo

Cartland, Siân P.; Genner, Scott W.; Zahoor, Amna; Kavurma, MaryM.
December 2016
International Journal of Molecular Sciences;Dec2016, Vol. 17 Issue 12, p2025
Academic Journal
Tumor necrosis-factor-related apoptosis-inducing ligand (TRAIL) has been implicated in angiogenesis; the growth of new blood vessels from an existing vessel bed. Our aim was to compare pro-angiogenic responses of TRAIL, vascular endothelial growth-factor-A (VEGF-A) and fibroblast growth-factor-2 (FGF-2) either separately (10 ng/mL) or in combination, followed by the assessment of proliferation, migration and tubule formation using human microvascular endothelial-1 (HMEC-1) cells in vitro. Angiogenesis was also measured in vivo using the Matrigel plug assay. TRAIL and FGF-2 significantly augmented HMEC-1 cell proliferation and migration, with combination treatment having an enhanced effect on cell migration only. In contrast, VEGF-A did not stimulate HMEC-1 migration at 10 ng/mL. Tubule formation was induced by all three factors, with TRAIL more effective compared to VEGF-A, but not FGF-2. TRAIL at 400 ng/mL, but not VEGF-A, promoted CD31-positive staining into the Matrigel plug. However, FGF-2 was superior, stimulating cell infiltration and angiogenesis better than TRAIL and VEGF-A in vivo. These findings demonstrate that each growth factor is more effective at different processes of angiogenesis in vitro and in vivo. Understanding how these molecules stimulate different processes relating to angiogenesis may help identify new strategies and treatments aimed at inhibiting or promoting dysregulated angiogenesis in people.


Related Articles

  • CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells. Król, Magdalena; Majchrzak, Kinga; Mucha, Joanna; Homa, Agata; Bulkowska, Małgorzata; Jakubowska, Arleta; Karwicka, Malwina; Pawłowski, Karol M.; Motyl, Tomasz // BMC Veterinary Research;5/1/2013, Vol. 9 Issue 1, Special section p1 

    Background: Tumor-associated macrophages (TAMs) have high impact on the cancer development because they can facilitate matrix invasion, angiogenesis, and tumor cell motility. It gives cancer cells the capacity to invade normal tissues and metastasize. The signaling of colony-stimulating factor-1...

  • Induction of RANTES by TWEAK/Fn14 Interaction in Human Keratinocytes. Jin, Long; Atsuhito; Nakayama, Masafumi; Yamaguchi, Noriko; Kojima, Yuko; Nakano, Nobuhiro; Tsuboi, Ryoli; Okumura, Ko; Yaqita, Hideo; Oqawa, Hideoki // Journal of Investigative Dermatology;May2004, Vol. 122 Issue 5, p1175 

    TNF-like weak inducer of apoptosis (TWEAK), a member of the tumor necrosis factor (TNF) family, is a multifunctional cytokine that regulate cellular proliferation, angiogenesis, inflammation, and apoptosis. In this study, we investigated the effect of TWEAK on human keratinocytes. Primary...

  • Osteoprotegerin and RANKL differentially regulate angiogenesis and endothelial cell function. McGonigle, Joseph S.; Giachelli, Cecilia M.; Scatena, Marta // Angiogenesis;Mar2009, Vol. 12 Issue 1, p35 

    Osteoprotegerin (OPG) a soluble tumor necrosis factor receptor family molecule protects endothelial cells from apoptosis in vitro and promotes neovascularization in vivo. In this study, we assessed the role of OPG and its ligands, receptor activator of nuclear factor-κB ligand (RANKL) and...

  • Tumor necrosis factor and cancer, buddies or foes? Xia WANG; Yong LIN // Acta Pharmacologica Sinica;Nov2008, Vol. 29 Issue 11, p1275 

    Tumor necrosis factor (TNF) is a multifunctional cytokine that plays important roles in diverse cellular events such as cell survival, proliferation, differentiation, and death. As a pro-inflammatory cytokine, TNF is secreted by inflammatory cells, which may be involved in...

  • VEGI, a new member of the TNF family activates Nuclear Factor-κB and c-Jun N-terminal kinase and modulates cell growth. Haridas, Valsala; Shrivastava, Anju; Su, Jeffrey; Yu, Guo-Liang; Ni, Jian; Liu, Ding; Chen, Su-Fang; Ni, Yansong; Ruben, Steve M; Gentz, Reiner; Aggarwal, Bharat B // Oncogene;11/11/99, Vol. 18 Issue 47, p6496 

    Recently a new member of the human tumor necrosis factor (TNF) family named as VEGI was reported. However, very little is known about the biological activities displayed by this cytokine. In this report, we show that in myeloid cells VEGI activated the transcription factor κB (NF-κB) as...

  • TNF receptor-associated factor 6 regulates proliferation, apoptosis, and invasion of glioma cells. Peng, Zhang; Shuangzhu, Yue; Yongjie, Jiang; Xinjun, Zhang; Ying, Liu // Molecular & Cellular Biochemistry;May2013, Vol. 377 Issue 1/2, p87 

    Tumor necrosis factor receptor-associated factor 6 (TRAF6), which plays an important role in inflammation and immune response, is an essential adaptor protein for the NF-κB (nuclear factor κB) signaling pathway. Recent studies have shown that TRAF6 played an important role in tumorigenesis...

  • TWEAK, a multifunctional cytokine in kidney injury. Sanz, Ana Belen; Sanchez-Niño, Maria D; Ortiz, Alberto // Kidney International;Oct2011, Vol. 80 Issue 7, p708 

    Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a cytokine of the TNF superfamily that activates the Fn14 receptor. TWEAK may regulate cell proliferation, cell death, cell differentiation, and inflammation. TWEAK and Fn14 are constitutively present in the kidney. Sources of TWEAK...

  • β-1,4-Galactosyltransferase I involved in Schwann cells proliferation and apoptosis induced by tumor necrosis factor-alpha via the activation of MAP kinases signal pathways. Yuan, Qin; Yang, Huiguang; Cheng, Chun; Li, Chunmiao; Wu, Xiujie; Huan, Weipeng; Sun, Huiqing; Zhou, Zhengming; Wang, Yingying; Zhao, Yunhong; Lu, Xiang; Shen, Aiguo // Molecular & Cellular Biochemistry;Jun2012, Vol. 365 Issue 1/2, p149 

    β-1,4-galactosyltransferase-I (β-1,4-GalT-I) plays a critical role in the initiation and maintenance of peripheral nervous system inflammatory reaction. However, the exact function of β-1,4-GalT-I in the regulation of SCs proliferation and apoptosis remains unclear. In this study, we...

  • Expression of TNF-α and Related Signaling Molecules in the Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis Patients. Raghav, Sunil Kumar; Gupta, Bhawna; Agrawal, Charu; Chaturvedi, Ved P.; Das, Hasi R. // Mediators of Inflammation;1/1/2006, Vol. 2006, Special section p1 

    We examined the role of tumor necrosis factor (TNF-α) and its related signaling intermediates leading to apoptosis/proliferation in the peripheral blood mononuclear cells (PBMCs) of RA patients. The constitutive expression of mRNA for TNF-α receptors (TNFR-I and TNFR-II) and the adapter...

  • TWEAK (tumor necrosis factor-like weak inducer of apoptosis) activates CXCL16 expression during renal tubulointerstitial inflammation. Izquierdo, María Concepción; Sanz, Ana B; Mezzano, Sergio; Blanco, Julia; Carrasco, Susana; Sanchez-Niño, María Dolores; Benito-Martín, Alberto; Ruiz-Ortega, Marta; Egido, Jesús; Ortiz, Alberto // Kidney International;Jun2012, Vol. 81 Issue 11, p1098 

    TWEAK (tumor necrosis factor-like weak inducer of apoptosis) is a TNF superfamily cytokine that activates the fibroblast growth factor-inducible 14 (Fn14) receptor. Transcriptional analysis of experimental kidney tubulointerstitial inflammation showed a correlation between an upregulation of the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics