TITLE

Screening the Expression Changes in MicroRNAs and Their Target Genes in Mature Cementoblasts Stimulated with Cyclic Tensile Stress

AUTHOR(S)
Liao Wang; Haikun Hu; Ye Cheng; Jianwei Chen; Chongyun Bao; Shujuan Zou; Gang Wu
PUB. DATE
December 2016
SOURCE
International Journal of Molecular Sciences;Dec2016, Vol. 17 Issue 12, p2024
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cementum is a thin layer of cementoblast-produced mineralized tissue covering the root surfaces of teeth. Mechanical forces, which are produced during masticatory activity, play a paramount role in stimulating cementoblastogenesis, which thereby facilitates the maintenance, remodeling and integrity of cementum. However, hitherto, the extent to which a post-transcriptional modulation mechanism is involved in this process has rarely been reported. In this study, a mature murine cementoblast cell line OCCM-30 cells (immortalized osteocalcin positive cementoblasts) was cultured and subjected to cyclic tensile stress (0.5 Hz, 2000 µstrain). We showed that the cyclic tensile stress could not only rearrange the cell alignment, but also influence the proliferation in an S-shaped manner. Furthermore, cyclic tensile stress could significantly promote cementoblastogenesis-related genes, proteins and mineralized nodules. From the miRNA array analyses, we found that 60 and 103 miRNAs were significantly altered 6 and 18 h after the stimulation using cyclic tensile stress, respectively. Based on a literature reviewand bioinformatics analyses, we found thatmiR-146b-5p and its target gene Smad4 play an important role in this procedure. The upregulation of miR-146b-5p and downregulation of Smad4 induced by the tensile stresswere further confirmed by qRT-PCR. The direct binding of miR-146b-5p to the three prime untranslated region (3'UTR) of Smad4 was established using a dual-luciferase reporter assay. Taken together, these results suggest an important involvement of miR-146b-5p and its target gene Smad4 in the cementoblastogenesis of mature cementoblasts.
ACCESSION #
120516848

 

Related Articles

  • Role of miR-140 in embryonic bone development and cancer. Green, Darrell; Dalmay, Tamas; Fraser, William D. // Clinical Science;Nov2015, Vol. 129 Issue 10, p863 

    Bone is increasingly viewed as an endocrine organ with key biological functions. The skeleton produces hormones and cytokines, such as FGF23 and osteocalcin, which regulate an extensive list of homoeostatic functions. Some of these functions include glucose metabolism, male fertility, blood cell...

  • Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices. Gjorevski, Nikolce; S. Piotrowski, Alexandra; Varner, Victor D.; Nelson, Celeste M. // Scientific Reports;7/17/2015, p11458 

    Collective cell migration drives tissue remodeling during development, wound repair, and metastatic invasion. The physical mechanisms by which cells move cohesively through dense three-dimensional (3D) extracellular matrix (ECM) remain incompletely understood. Here, we show directly that...

  • EFFECTS OF ADHESION AND STRETCHING ON FAILURE MECHANISMS AND OPTICAL PROPERTIES OF ORGANIC SOLAR CELLS. Oyewole, O. K.; Asare, J.; Oyewole, D. O.; Agyei-Tuffour, B.; Anye, V. C.; Kana, M. G. Zebaze; Soboyejo, W. O. // Advanced Materials Research;2016, Vol. 1132, p89 

    This paper presents the results of a study of the adhesion and optical properties of layered structures that are relevant to stretchable organic solar cells. A combination of modeling and experiments is used to investigate the effects of adhesion and stretching on failure mechanisms and optical...

  • Effects of Fat Content and Solid Fat Content on Caramel Texture Attributes. Mendenhall, Heather; Hartel, Richard // Journal of the American Oil Chemists' Society (JAOCS);Sep2016, Vol. 93 Issue 9, p1191 

    Fat plays an important role in caramel quality attributes, yet there is very little published work on how fat type and level influence caramel characteristics. Fat content was increased from 0 to 20 % to determine the effects of total fat content on caramel texture attributes such as cold flow,...

  • Tenogenic differentiation of equine adipose-tissue-derived stem cells under the influence of tensile strain, growth differentiation factors and various oxygen tensions. Raabe, Oksana; Shell, K.; Fietz, D.; Freitag, C.; Ohrndorf, A.; Christ, H.; Wenisch, S.; Arnhold, S. // Cell & Tissue Research;Jun2013, Vol. 352 Issue 3, p509 

    Mesenchymal stem cells have become extremely interesting for regenerative medicine and tissue engineering in the horse. Stem cell therapy has been proven to be a powerful and successful instrument, in particular for the healing of tendon lesions. We pre-differentiated equine...

  • Electrospinning of poly ( ε-caprolactone-co-lactide)/Pluronic blended scaffolds for skin tissue engineering. Liu, Ning-hua; Pan, Jian-feng; Miao, Yue-E; Liu, Tian-xi; Xu, Feng; Sun, Hui // Journal of Materials Science;Oct2014, Vol. 49 Issue 20, p7253 

    For skin tissue engineering, an ideal scaffold should mimic the natural extracellular matrix of the native skin. In this study, we reported a novel elastic sub-micron fiber scaffold blending poly ( ε-caprolactone-co-lactide) (PLCL) and Pluronic at different ratios by electrospinning. PLCL and...

  • Growth control by cell wall pectins. Wolf, Sebastian; Greiner, Steffen // Protoplasma;Jun2012, Vol. 249, p169 

    Plant cell growth is controlled by the balance between turgor pressure and the extensibility of the cell wall. Several distinct classes of wall polysaccharides and their interactions contribute to the architecture and the emergent features of the wall. As a result, remarkable tensile strength is...

  • Collagen-Based Scaffolds for Potential Application of Heart Valve Tissue Engineering. Qi Chen; Bruyneel, Arne; Clarke, Kieran; Carr, Carolyn; Czernuszka, Jan // Journal of Tissue Science & Engineering;Special2012, Vol. 3 Issue S, Special section p1 

    Collagen, glycosaminoglycans and elastin are the major components of heart valves. This work examines the roles of the composition of the collagen-based scaffolds on the microstructures, mechanical properties, and bioactivities. Scaffolds (with up to 80% elastin or 50% chondroitin-4-sulfate)...

  • Manipulating cinnamyl alcohol dehydrogenase (CAD) expression in flax affects fibre composition and properties. Preisner, Marta; Kulma, Anna; Zebrowski, Jacek; Dymińska, Lucyna; Hanuza, Jerzy; Arendt, Malgorzata; Starzycki, Michal; Szopa, Jan // BMC Plant Biology;2014, Vol. 14 Issue 1, p1 

    Background In recent decades cultivation of flax and its application have dramatically decreased. One of the reasons for this is unpredictable quality and properties of flax fibre, because they depend on environmental factors, retting duration and growing conditions. These factors have...

  • Restoration of tensile strength in bark samples of Ficus benjamina due to coagulation of latex during fast self-healing of fissures. Bauer, Georg; Speck, Thomas // Annals of Botany;Mar2012, Vol. 109 Issue 4, p807 

    Background and Aims The functions of plant latex have been discussed for a long time. Today, many studies support a defence mechanism as being its main function. A role as a self-healing mechanism was never attributed to the coagulation of latex. In this study we quantified the contribution of...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics