Secondary Structure Adopted by the Gly-Gly-X Repetitive Regions of Dragline Spider Silk

Gray, Geoffrey M.; van der Vaart, Arjan; Chengchen Guo; Jones, Justin; Onofrei, David; Cherry, Brian R.; Lewis, Randolph V.; Yarger, Jeffery L.; Holland, Gregory P.
December 2016
International Journal of Molecular Sciences;Dec2016, Vol. 17 Issue 12, p2023
Academic Journal
Solid-state NMR and molecular dynamics (MD) simulations are presented to help elucidate the molecular secondary structure of poly(Gly-Gly-X), which is one of the most common structural repetitive motifs found in orb-weaving dragline spider silk proteins. The combination of NMR and computational experiments provides insight into the molecular secondary structure of poly(Gly-Gly-X) segments and provides further support that these regions are disordered and primarily non-β-sheet. Furthermore, the combination of NMR and MD simulations illustrate the possibility for several secondary structural elements in the poly(Gly-Gly-X) regions of dragline silks, including β-turns, 310-helicies, and coil structures with a negligible population of α-helix observed.


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