TITLE

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

AUTHOR(S)
Lei Cui; Hongtao Hu; Wei Wei; Weimin Wang; Hong Liu
PUB. DATE
December 2016
SOURCE
International Journal of Molecular Sciences;Dec2016, Vol. 17 Issue 12, p1972
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.
ACCESSION #
120516795

 

Related Articles

  • Development of Novel antimiRzymes for Targeted Inhibition of miR-21 Expression in Solid Cancer Cells. Larcher, Leon M.; Wang, Tao; Veedu, Rakesh N. // Molecules;7/1/2019, Vol. 24 Issue 13, p2489 

    MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the regulation of gene expression. Previous reports showed an over-expression of miRNA-21 (miR-21) in various cancer cells, and its up-regulation is closely related to cancer initiation, proliferation and metastasis. In this work,...

  • The role of small RNAs on phenotypes in reciprocal hybrids between Solanum lycopersicum and S. pimpinellifolium. Junxing Li; Qian Sun; Ningning Yu; Jiajin Zhu; Xiaoxia Zou; Zhenyu Qi; Muhammad Awais Ghani; Liping Chen // BMC Plant Biology;2014, Vol. 14 Issue 1, p2 

    Background Reciprocal hybrids showing different phenotypes have been well documented in previous studies, and many factors accounting for different phenotypes have been extensively investigated. However, less is known about whether the profiles of small RNAs differ between reciprocal hybrids and...

  • Identification of differences in microRNA transcriptomes between porcine oxidative and glycolytic skeletal muscles. Yingkai Liu; Mingzhou Li; Jideng Ma; Jie Zhang; Chaowei Zhou; Tao Wang; Xiaolian Gao; Xuewei Li // BMC Molecular Biology;2013, Vol. 14 Issue 1, p1 

    Background: MicroRNAs (miRNAs) are a type of non-coding small RNA ~22 nucleotides in length that regulate the expression of protein coding genes at the post-transcriptional level. Glycolytic and oxidative myofibers, the two main types of skeletal muscles, play important roles in metabolic health...

  • MicroRNA Transcriptome of Poly I:C-Stimulated Peripheral Blood Mononuclear Cells Reveals Evidence for MicroRNAs in Regulating Host Response to RNA Viruses in Pigs. Jiying Wang; Yanping Wang; Haifei Wang; Jianfeng Guo; Huaizhong Wang; Ying Wu; Jianfeng Liu // International Journal of Molecular Sciences;Oct2016, Vol. 17 Issue 10, p1601 

    MicroRNAs (miRNAs) are one family of small noncoding RNAs that function to modulate the activity of specific mRNA targets in animals. To understand the role of miRNAs in regulating genes involved in the host immune response to RNA viruses, we profiled and characterized the miRNAs of swine...

  • Knowledge-based analysis of functional impacts of mutations in microRNA seed regions. Bhattacharya, Anindya; Cui, Yan // Journal of Biosciences;Oct2015, Vol. 40 Issue 4, p791 

    MicroRNAs are a class of important post-transcriptional regulators. Genetic and somatic mutations in miRNAs, especially those in the seed regions, have profound and broad impacts on gene expression and physiological and pathological processes. Over 500 SNPs were mapped to the miRNA seeds, which...

  • Large non-coding RNA in pluripotency. Le Bot, Nathalie // Nature Cell Biology;May2013, Vol. 15 Issue 5, p449 

    The article presents a study by Liu and colleagues which showed that linc-RoR, a big non-coding RNA, increases pluripotency factors in embryonic stem cells (ESCs) and keeps microRNAs away from their target sites.

  • Novel roles of non-coding brain RNAs in health and disease. Soreq, Hermona // Frontiers in Molecular Neuroscience;Jun2014, Vol. 7, p1 

    An introduction is presented which outlines the theme of the issue focusing on non-coding RNA and microRNA research.

  • The role of microRNA-30c-2* as an anti-angiogenic mediator. Shantikumar, S.; Caporali, A.; Meloni, M.; Marchetti, M.; Howard, L.; Martelli, F.; Emanueli, C. // Proceedings of the Physiological Society;2013, p986P 

    Purpose: MicroRNAs (miRs) are small noncoding RNAs which negatively regulate the expression of targeted mRNA transcripts. Some miRs are already known to modulate angiogenesis. The p75 neurotrophin receptor (p75NTR) is upregulated in endothelial cells (ECs) of diabetic models and contributes to...

  • Identification of lncRNA MEG3 Binding Protein Using MS2-Tagged RNA Affinity Purification and Mass Spectrometry. Liu, Shanshan; Zhu, Juanjuan; Jiang, Taifeng; Zhong, Yiran; Tie, Yi; Wu, Yongge; Zheng, Xiaofei; Jin, Yinghua; Fu, Hanjiang // Applied Biochemistry & Biotechnology;Aug2015, Vol. 176 Issue 7, p1834 

    Long noncoding RNAs (lncRNAs) are nonprotein coding transcripts longer than 200 nucleotides. Recently in mammals, thousands of long noncoding RNAs have been identified and studied as key molecular players in different biological processes with protein complexes. As a long noncoding RNA,...

  • Microarray profiling of long non-coding RNA (lncRNA) associated with hypertrophic cardiomyopathy. Wei Yang; Yuan Li; Fawei He; Haixiang Wu // BMC Cardiovascular Disorders;Jul2015, Vol. 15 Issue 1, p1 

    Background: Hypertrophic cardiomyopathy (HCM) is an inherited disorder with around 1400 known mutations; however the molecular pathways leading from genotype to phenotype are not fully understood. LncRNAs, which account for approximately 98 % of human genome, are becoming increasingly...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics