Antimicrobial potential and taxonomic investigation of piezotolerant Streptomyces sp. NIOT-Ch-40 isolated from deep-sea sediment

Padmanaban, Vishnu; Sekar, Ashok; Verma, Pankaj; Venkatabaskaran, Srividhyalakshmi; Keppayan, Thirupathi; Gopal, Dharani; Ramalingam, Kirubagaran
February 2017
World Journal of Microbiology & Biotechnology;Feb2017, Vol. 33 Issue 2, p1
Academic Journal
Microbial-derived natural products from extreme niches such as deepsea are known to possess structural and functional novelty. With this background, the present study was designed to investigate the bioprospecting potential and systematics of a deep-sea derived piezotolerant bacterial strain NIOT-Ch-40, showing affiliation to the genus Streptomyces based on 16S RNA gene similarity. Preliminary screening for the presence of biosynthetic genes like polyketide synthase I, polyketide synthase II, non ribosomal peptide synthase, 3-amino-5-hydroxybenzoic acid synthase and spiroindimicin followed by antibacterial activity testing confirmed the presence of potent bioactivity. The secondary metabolites produced during fermentation in Streptomyces broth at 28 °C for 7 days were extracted with ethyl acetate. The extract exhibited a specific inhibitory activity against Gram-positive bacteria and was significantly effective ( p < 0.0001) against methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration and minimum bactericidal concentration against MRSA was 1.5 µg/mL, which was statistically significant in comparison with erythromycin. A multifaceted analysis of the Streptomyces spp. was carried out to delineate the strain NIOT-Ch-40 at a higher resolution which includes morphological, biochemical and molecular studies. Piezotolerance studies and comparison of fatty acid profiles at high pressures revealed that it could be considered as one of the taxonomic markers, especially for the strains isolated from the deep sea environments. In conclusion, the observation of comparative studies with reference strains indicated towards the strain NIOT-Ch-40 as an indigenous marine piezotolerant Streptomyces sp. with a higher probability of obtaining novel bioactive metabolites.


Related Articles


    In this work, actinomycetes isolates were isolated from rock wall and speleothem surfaces and soil samples of 19 karstic caves in Turkey. Out of 290 isolates isolated, 180 isolates (62%) exhibited antimicrobial activity against a panel of four bacteria, two yeasts and four filamentous fungi in...

  • Antimicrobial Resistance in Ophthalmology. Hwang, David G. // Review of Ophthalmology;Apr2012 Supplement, p6 

    The article discusses research on the mechanism of methicillin and fluoroquinolone resistance by ocular pathogens and its clinical implications. It cites the Ocular TRUST 2, a surveillance effort focusing on ocular isolates, which showed that 54% of the 155 ocular Staphylococcus aureus isolates...

  • Community-acquired methicillin-resistant Staphylococcus aureus: community transmission, pathogenesis, and drug resistance. Yamamoto, Tatsuo; Nishiyama, Akihito; Takano, Tomomi; Yabe, Shizuka; Higuchi, Wataru; Razvina, Olga; Shi, Da // Journal of Infection & Chemotherapy;Aug2010, Vol. 16 Issue 4, p225 

    Methicillin-resistant Staphylococcus aureus (MRSA) is able to persist not only in hospitals (with a high level of antimicrobial agent use) but also in the community (with a low level of antimicrobial agent use). The former is called hospital-acquired MRSA (HA-MRSA) and the latter...

  • Relative prevalence of methicilline resistant Staphylococcus aureus and its susceptibility pattern in Mulago Hospital, Kampala, Uganda. OJULONG, J.; MWAMBU, T. P.; JOLOBA, M.; BWANGA, F.; KADDU-MULINDWA, D. H. // Tanzania Journal of Health Research;Oct2009, Vol. 11 Issue 3, p149 

    Methicilline resistant Staphylococcus aureus (MRSA) strains are becoming increasingly multiresistant, and have recently developed resistance to vancomycin, which has been used successfully to treat MRSA for many years. In-vitro determination of resistance patterns of S. aureus is critical in...

  • Forever Unprepared � The Predictable Unpredictability of Pathogens. Sepkowitz, Kent A. // New England Journal of Medicine;7/9/2009, Vol. 361 Issue 2, p120 

    The author offers insights on the predictable unpredictability of the pathogens methicillin-resistant Staphylococcus aureus (MRSA) and the pre-swine edition of influenza virus. He warns about the spread of community-acquired MRSA into hospitals and clinics and the appearance of...

  • Methicillin-Resistant Staphylococcus aureus ST9 in Pigs in Thailand. Larsen, Jesper; Imanishi, Maho; Hinjoy, Soawapak; Tharavichitkul, Prasit; Duangsong, Kwanjit; Davis, Meghan F.; Nelson, Kenrad E.; Larsen, Anders R.; Skov, Robert L. // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    Background: Methicillin-resistant Staphylococcus aureus (MRSA) is an important nosocomial and community-associated pathogen. Recently, livestock-associated MRSA (LA-MRSA) has emerged and disseminated in Europe and North America and now constitutes a considerable zoonotic burden in humans with...

  • In-vitro activity of tigecycline against methicillin-resistant Staphylococcus aureus Isolated from wounds of burn patients in Tripoli-Libya. Zorgani, Abdulazziz; Elahmer, Omar; Ziglam, Hisham; Ghenghesh, Khalifa Sifaw // Journal of Microbiology & Infectious Diseases;Sep2012, Vol. 2 Issue 3, p109 

    Objectives: Tigecycline is a new glycylcycline group antibiotic with broad-spectrum activity. In the present study we report on in vitro activity of tigecycline as well as the comparator antimicrobials linezolid and quinupristin/dalfopristin against methicillin-susceptible Staphylococcus aureus...

  • Not Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA)! A Clinician's Guide to Community MRSA - Its Evolving Antimicrobial Resistance and Implications for Therapy. Chua, Kyra; Laurent, Frederic; Coombs, Geoffrey; Grayson, M. Lindsay; Howden, Benjamin P. // Clinical Infectious Diseases;Jan2011, Vol. 52 Issue 1, p99 

    There is significant diversity in methicillin-resistant Staphylococcus aureus (MRSA) clones arising in the community worldwide, with considerable geographical differences in typical antimicrobial resistance profiles. Many community clones of MRSA have a non–multidrug resistant...

  • Linezolid for endocarditis: a case series of 14 patients. Tascini, Carlo; Bongiorni, Maria Grazia; Doria, Roberta; Polidori, Marina; Iapoce, Riccardo; Fondelli, Serena; Tagliaferri, Enrico; Soldati, Ezio; Di Paolo, Antonello; Leonildi, Alessandro; Menichetti, Francesco // Journal of Antimicrobial Chemotherapy (JAC);Mar2011, Vol. 66 Issue 3, p679 

    The article discusses several cases of endocarditis treated with linezolid. Linezolid is considered an antibiotic for treatment of complicated skin and soft tissue infections and pneumonia. The case series include 12 patients at Cisanello Hospital in Pisa, Italy who were treated with linezolid...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics