Inhibitory roles of miR-320 in osteosarcoma via regulating E2F1

Haojie Wu; Weihua Li; Minghui Zhang; Shutao Zhu; Dengfeng Zhang; Xiao Wang
October 2016
Journal of Cancer Research & Therapeutics;2016 Special Issue, Vol. 12, pC68
Academic Journal
Objectives: Osteosarcoma (OsC) is the most common primary bone malignant tumor with lower incidence and high degree of malignancy, but the exact mechanism remains unknown. More evidence demonstrated microRNAs (miRNAs) could contribute to tumor progression. In this study, we investigated the expression and functions of miR-320 in OsC cells. Materials and Methods: miR-320 expression levels in several human OsC cell lines and human normal osteoblastic cell line were tested by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). U2OS cells were transfected with miR-320 mimics or negative control oligos. MTT assay and cell flow cytometry assay by PI staining were performed to access the cell growth rate. Bioinformatic prediction and luciferase assays were used to identify the predicted target E2F1. qRT-PCR and Western blot were performed to access the molecular alteration of E2F1. Results: miR-320 was decreased in human OsC cell lines. Heterogeneous expression of miR-320 inhibited cell proliferation and induced cell cycle arrest. Besides, we proved that miR-320 could directly regulate the expression of E2F1 in U2OS cells. Conclusion: These data suggested that miR-320 regulates the proliferation and cell cycle by targeting E2F1 in human OsC progression.


Related Articles

  • Downregulation of microRNA-210 inhibits osteosarcoma growth in vitro and in vivo. CHANGJIAN LIU; XIN TANG // Molecular Medicine Reports;2015, Vol. 12 Issue 3, p3674 

    MicroRNA-210 (miR-210), the master hypoxamir, has various roles in the development of certain cancer types. It has been reported that miR-210 expression was upregulated in patients with osteosarcoma (OS). However, little is known regarding its role in the development of human OS. In the present...

  • AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression. Zhang, Haiyun; Liu, Yujie; Xu, Kehan; Mao, Kai; Han, Weidong; Xu, Feifan; Wan, Wei; Sun, Yajun // Cancer Management & Research;Nov2019, Vol. 11, p9913 

    Introduction: Amphiphysin 1 (AMPH-1) is involved in endocytosis, and its expression is upregulated in osteosarcoma compared with osteofibrous dysplasia. Methods: We investigated the role of AMPH-1 in osteosarcoma cells via both gain-of-function and loss-of-function experiments. Results:...

  • mi-374a promotes the proliferation of osteosarcoma cell proliferation by targeting Axin2. Tan Lu; Chao Zhang; Ming-Xiang Chai; Yong-Bo An; Jin-Ling Jia // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 9, p10776 

    Background: MicroRNA-374a (mi-374a) has been implicated in several cancers. However, its role in osteosarcoma (OS) remains unclear. Thus the aim of this study was to investigate its expression and role in progression of OS. Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect...

  • MicroRNA-665 suppressed the invasion and metastasis of osteosarcoma by directly inhibiting RAB23. Chenhui Dong; Quanyin Du; Zimin Wang; Yu Wang; Siyu Wu; Aimin Wang // American Journal of Translational Research;2016, Vol. 8 Issue 11, p4975 

    MicroRNAs (miRNAs) are small, short and noncoding RNAs that regulate gene expression posttranscriptionally. Increasing evidences have demonstrated that deregulated expression of miRNAs is found in osteosarcoma. In this study, we demonstrated that miR-665 was downregulated in osteosarcoma tissues...

  • MicroRNA-125a Regulates Cell Proliferation Via Directly Targeting E2F2 in Osteosarcoma. Tao, Tieying; Shen, Qinrong; Luo, Jianmin; Xu, Yang; Liang, Wenqing // Cellular Physiology & Biochemistry (Karger AG);Oct2017, Vol. 43 Issue 2, p768 

    Background/Aims: Increasing evidence has shown that miR-125a plays important role in human cancer progression. However, little is known about the function of miR-125a in osteosarcoma. Methods: The expression of miR-125a in osteosarcoma tissues and cell lines were examined by qRT-PCR. The...

  • Downregulation of microRNA-586 Inhibits Proliferation, Invasion and Metastasis and Promotes Apoptosis in Human Osteosarcoma U2-OS Cell Line. Yang, Lei; Liu, Zong-Ming; Rao, Yan-Wei; Cui, Shao-Qian; Wang, Huan; Jia, Xiao-Jing // Cytogenetic & Genome Research;Jan2016, Vol. 146 Issue 4, p268 

    In this study, we aim to examine the association of microRNA-586 (miR-586) with osteosarcoma (OS) cell proliferation, apoptosis, invasion, and metastasis. U2-OS cell lines were divided into 4 groups: an miR-586 group, anti-miR-586 group, control group (empty plasmid) and blank group (no...

  • Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7. Xue, Zhen; Zhao, Jindong; Niu, Liyuan; An, Gang; Guo, Yashan; Ni, Linying // PLoS ONE;May2015, Vol. 10 Issue 5, p1 

    Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression and function of miR-300 in osteosarcoma is still unknown. In our study, we found that the expression of miR-300 was up-regulated in osteosarcoma tissues and...

  • MicroRNA-20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression. WENKUN ZHUO; WEIMING GE; GUOLIN MENG; SHUAIJUN JIA; XIANG ZHOU; JIAN LIU // Molecular Medicine Reports;2015, Vol. 12 Issue 4, p4989 

    MicroRNAs (miRNAs) are crucial in cancer development. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) remain largely uncharacterized. The present study investigated the role of miR-20a in OS cell proliferation. It was determined that miR-20a expression is markedly upregulated...

  • MicroRNA 520d-3p inhibits gastric cancer cell proliferation, migration, and invasion by downregulating EphA2 expression. Li, Ruixin; Yuan, Weijie; Chen, Zihua; Mei, Wenjuan; Yang, Keda // Molecular & Cellular Biochemistry;Nov2014, Vol. 396 Issue 1/2, p295 

    Aberrant expression of microRNAs (miRNAs) has been shown to play important roles in cancer progression as a result of changes in expression of their target genes. In this study, we investigated the roles of miR- 520d- 3p on gastric cancer (GC) cell proliferation, migration, and invasion, and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics