TITLE

Inhibitory roles of miR-320 in osteosarcoma via regulating E2F1

AUTHOR(S)
Haojie Wu; Weihua Li; Minghui Zhang; Shutao Zhu; Dengfeng Zhang; Xiao Wang
PUB. DATE
October 2016
SOURCE
Journal of Cancer Research & Therapeutics;2016 Special Issue, Vol. 12, pC68
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objectives: Osteosarcoma (OsC) is the most common primary bone malignant tumor with lower incidence and high degree of malignancy, but the exact mechanism remains unknown. More evidence demonstrated microRNAs (miRNAs) could contribute to tumor progression. In this study, we investigated the expression and functions of miR-320 in OsC cells. Materials and Methods: miR-320 expression levels in several human OsC cell lines and human normal osteoblastic cell line were tested by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). U2OS cells were transfected with miR-320 mimics or negative control oligos. MTT assay and cell flow cytometry assay by PI staining were performed to access the cell growth rate. Bioinformatic prediction and luciferase assays were used to identify the predicted target E2F1. qRT-PCR and Western blot were performed to access the molecular alteration of E2F1. Results: miR-320 was decreased in human OsC cell lines. Heterogeneous expression of miR-320 inhibited cell proliferation and induced cell cycle arrest. Besides, we proved that miR-320 could directly regulate the expression of E2F1 in U2OS cells. Conclusion: These data suggested that miR-320 regulates the proliferation and cell cycle by targeting E2F1 in human OsC progression.
ACCESSION #
119088988

 

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