TITLE

Comparison of enteric protozoan infections in four Australian hospitals: variable tests and variable results

AUTHOR(S)
FLETCHER-LARTEY, STEPHANIE M.; ANDRESEN, DAVID; VAN HAL, SEBASTIAN; MERIF, JUAN; STARK, DAMIEN; RAWLINSON, WILLIAM; HARKNESS, JOHN; ELLIS, JOHN
PUB. DATE
September 2016
SOURCE
Parasitology Open;9/9/2016, Vol. 2, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
There is limited evidence of the prevalence of enteric protozoon infections in developed settings. We estimated the prevalence of enteric protozoa and evaluated the outcome of testing algorithms used in hospital settings in Sydney, Australia. This retrospective study assessed microbiological data from four public clinical laboratories. Pooled data from the four hospitals revealed the most common enteric protozoon detected was Blastocystis spp. in an average of 5.4% of cases, followed by Giardia intestinalis (1.1%) and Dientamoeba fragilis (0.8%). Protozoon detection rates between hospitals were significantly different and could be based on multiple factors. The modified iron haematoxylin staining method, consistently detected higher rates of Blastocystis spp., and G. intestinalis in comparison with microscopy of wet preparations, as well as higher rates of G. intestinalis and Cryptosporidium when compared with enzyme immunoassay. The study concludes that there are multiple factors that contribute to the variability in protozoa detection rates in metropolitan hospitals, including widespread variability in the testing protocols for enteric protozoa, individual and population characteristics. A gold standard approach for diagnosis of enteric protozoa is recommended. Molecular diagnostic methods such as polymerase chain reaction would provide consistency across laboratories and yield more reliable estimates of the actual prevalence of enteric protozoa.
ACCESSION #
118067158

 

Related Articles

  • Detection of concurrent infection of dairy cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods. Fayer, Ronald; Santin, Monica; Macarisin, Dumitru // Parasitology Research;Sep2012, Vol. 111 Issue 3, p1349 

    Of fecal specimens examined from 47 dairy cattle ranging in age from neonates to multiparous cows, 9, 10, 24, and 17 were positive for Blastocystis spp., Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi, respectively, as determined by PCR. Eight 3- to 5-month-old cattle were...

  • Detection of Cryptosporidium and Giardia (oo)cysts by IFA, PCR and LAMP in surface water from Rasht, Iran. Mahmoudi, Mohammad-Reza; Kazemi, Bahram; Mohammadiha, Anita; Mirzaei, Asad; Karanis, Panagiotis // Transactions of the Royal Society of Tropical Medicine & Hygiene;Aug2013, Vol. 107 Issue 8, p511 

    Background Cryptosporidium and Giardia in water supplies is acknowledged as a public health problem. In the present study, we applied immunofluorescence assay (IFA), PCR and loop-mediated isothermal amplification (LAMP) for the detection of the two protozoa. Methods Over a period of 12 months,...

  • Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness. Morris Jones; Christopher Whipps; Robert Ganac; N. Hudson; Kenneth Boroom // Parasitology Research;Jan2009, Vol. 104 Issue 2, p341 

    Abstract  Chronic gastrointestinal illness of unknown etiology is a significant problem in the United States. Using a real-time LightCycler PCR assay we detected Blastocystis in nine patients from a metropolitan area of Corvallis, Oregon who presented with diarrhea, abdominal...

  • Proteaese activity of Blastocystis hominis subtype3 in symptomatic and asymptomatic patients. Abdel-Hameed, Dina; Hassanin, Omayma // Parasitology Research;Aug2011, Vol. 109 Issue 2, p321 

    Despite accumulating evidence indicating that Blastocystis hominis is pathogenic and that cysteine proteases are involved in its pathogenesis, few researches discussed the protease activity of B. hominis genetic subtypes. Therefore, the present study aims to identify the underlying pathogenic...

  • A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis. Funda Dogruman-Al; Hande Dagci; Hisao Yoshikawa; Özgur Kurt; Mete Demirel // Parasitology Research;Aug2008, Vol. 103 Issue 3, p685 

    Abstract   Blastocystis hominis is one of the most common eukaryotic organisms in the intestinal tract of humans, while its pathogenic potential is still controversial. A total of 286 stool samples obtained from adult and pediatric patients with or without gastrointestinal symptoms in two...

  • Epidemiology and Molecular Relationships of Cryptosporidium spp. in People, Primates, and Livestock from Western Uganda. Salyer, Stephanie J.; Gillespie, Thomas R.; Rwego, Innocent B.; Chapman, Colin A.; Goldberg, Tony L. // PLoS Neglected Tropical Diseases;Apr2012, Vol. 6 Issue 4, p1 

    Background: Cryptosporidium is one of the most common parasitic diarrheal agents in the world and is a known zoonosis. We studied Cryptosporidium in people, livestock, and non-human primates in the region of Kibale National Park, Uganda. Land use change near the park has resulted in fragmented...

  • Intestinal parasitoses in a tertiary-care hospital located in a non-endemic setting during 2006-2010. Calderaro, Adriana; Montecchini, Sara; Rossi, Sabina; Gorrini, Chiara; De Conto, Flora; Medici, Maria Cristina; Chezzi, Carlo; Arcangeletti, Maria Cristina // BMC Infectious Diseases;2014, Vol. 14 Issue 1, p1 

    Background The aim of this study was to assess the epidemiology of intestinal parasitoses during a 5-year period in patients attending a tertiary-care hospital in a non-endemic setting. Methods In the period 2006-2010, 15,752 samples from 8,886 patients with clinically suspected parasitosis were...

  • Association of Blastocystis hominis genetic subtypes with urticaria. Hameed, Dina M.; Hassanin, Omayma M.; Zuel-Fakkar, Nehal Mohamed // Parasitology Research;Mar2011, Vol. 108 Issue 3, p553 

    lthough intestinal parasites are a possible cause of skin disorders, there are few case reports concerning the role of Blastocystis hominis in urticaria. To clarify this association, we determined the frequency of B. hominis genetic subtype in urticarial patients by stool culture and polymerase...

  • Molecular characterization of Cryptosporidium in children in Oyo State, Nigeria: implications for infection sources. Ayinmode, Adekunle; Fagbemi, Benjamin; Xiao, Lihua // Parasitology Research;Jan2012, Vol. 110 Issue 1, p479 

    A study was conducted to detect and identify Cryptosporidium spp. in 43 children from Oyo State, Nigeria. Using nested polymerase chain reaction, 11.6% of the children were identified as positive for Cryptosporidium spp. Restriction fragment length polymorphism analysis and DNA sequencing of the...

  • Assessment of molecular methods as a tool for detecting pathogenic protozoa isolated from water bodies. Adamska, M.; Sawczuk, M.; Kolodziejczyk, L.; Skotarczak, B. // Journal of Water & Health;2015, Vol. 13 Issue 4, p953 

    Several species belong to the Cryptosporidium and Giardia genus, the main parasitic protozoa occurring in water, but only some of them are infectious to humans. We investigated the occurrence of Cryptosporidium and Giardia and identified their species in the water samples collected from natural...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics