lncRNA91H 在结直肠癌患者中的表达及其与临床病理特征 和预后的关系

吉志武; 陈福军; 杨帆; 倪健; 朱小文; 于海涛; 付岩; 赵斌
June 2016
Progress in Modern Biomedicine;Jun2016, Vol. 16 Issue 18, p3511
Academic Journal
Objective: To investigate the expression of long noncoding RNA(lncRNA) 91H and its correlation with clinical pathological features and prognosis in patients with colorectal cancer(CRC). Methods: A total of 80 specimens(including cancer tissues and para cancer tissues) of patients with CRC, who underwent CRC radical resection in First Affiliated Hospital of Jiamusi University from January 2010 to January 2012, were selected. The expression of lncRNA91 in cancer tissues and para cancer tissues was detected by quantitative real-time PCR.The disturbance sequence was set; the cell proliferation rate was detected by thiazole blue(MTT) and cell apoptosis rate was detected by flow cytometry; the correlation between lncRNA91H and prognosis was analyzed by COX. Results: The relative expression amount of lncRNA91H in cancer tissues and para cancer tissues was (1.83± 0.14) and (0.36± 0.07) respectively, the difference was statistically significant (P<0.05). The expressions of lncRNA91H in different TNM stage, tumor metastasis and invasion depth were statistically different (P<0.05). Multivariate COX analysis showed that TNM stage, tumor metastasis, invasion depth and expression of lncRNA91H were the independent risk factors of prognosis of CRC (P<0.05). The apoptosis rate of negative control group was(5.67± 1.23)%, and the apoptosis rate of transfected lncRNA91H cells was(25.37± 0.89)%, the difference was statistically significant (P<0.05).Conclusion: Lnc RNA91H can be used as a specific index of prognosis of CRC patients, which is closely correlated with the clinical pathological features of TNM stage, tumor metastasis and invasion depth.


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