TITLE

Calcium Imaging of Murine Thoracic Aorta Endothelium by Confocal Microscopy Reveals Inhomogeneous Distribution of Endothelial Cells Responding to Vasodilator Agents

AUTHOR(S)
Marie, Isabelle; Bény, Jean-Louis
PUB. DATE
June 2002
SOURCE
Journal of Vascular Research;2002, Vol. 39 Issue 3, p260
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The aim of the study was to assess, in intact murine thoracic aorta in vitro, the distribution of endothelial cells responsive to endothelium-dependent vasodilators ACh, ATP, bradykinin and substance P, using laser line confocal microscopy in combination with two Ca[sup 2+] fluorescent dyes, Fluo-4 and Fura-red. We observed that 82 ± 3% of endothelial cells responded to ATP, 33 ± 5% to Ach, whereas less than 3% of them responded to bradykinin or substance P. In order to determine whether the findings of pharmacological tests agree with confocal microscopy data, endothelium-dependent vasodilators induced relaxation was evaluated using isometric tension measurement. We show a marked correlation between a higher number of activated endothelial cells, using confocal microscopy, and a greater degree of endothelium-dependent relaxation using isometric tension measurement (p = 0.00286). Our results suggest that endothelial cells responding to endothelium-dependent vasodilators are not homogeneously distributed in intact murine thoracic aorta. This could be due to nonhomogeneous distribution of surface receptors or to differences in post-receptor coupling mechanisms.Copyright © 2002 S. Karger AG, Basel
ACCESSION #
11636908

 

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