Nanotechnology for neuronal ion channels

Lehmann-Horn F; Jurkat-Rott K; Lehmann-Horn, F; Jurkat-Rott, K
November 2003
Journal of Neurology, Neurosurgery & Psychiatry;Nov2003, Vol. 74 Issue 11, p1466
Academic Journal
journal article
Ion channels provide the basis for the regulation of electrical excitability in the central and peripheral nervous systems. This review deals with the techniques that make the study of structure and function of single channel molecules in living cells possible. These are the patch clamp technique, which was derived from the conventional voltage clamp method and is currently being developed for automated and high throughput measurements; and fluorescence and nano-techniques, which were originally applied to non-biological surfaces and are only recently being used to study cell membranes and their proteins, especially in combination with the patch clamp technique. The characterisation of the membrane channels by techniques that resolve their morphological and physical properties and dynamics in space and time in the nano range is termed nanoscopy.


Related Articles

  • Dynamics of Functioning of Voltage-Operated Ion Channels in the Dendrite Plasma Membrane. Magura, I.; Bogdanova, N.; Dolgaya, E. // Neurophysiology;Nov2013, Vol. 45 Issue 5/6, p495 

    Dendrites provide the analysis and transmission of numerous and variable synaptic signals to the soma and axon hillock of nerve cells. According to generally accepted concepts, generation of action potentials (APs) is initiated precisely in the latter cell compartment. The problem of the...

  • Ghrelin modulates the activity and synaptic input organization of midbrain dopamine neurons while promoting appetite. Abizaid, Alfonso; Zhong-Wu Liu; Andrews, Zane B.; Shanabrough, Marya; Borok, Erzsebet; Elsworth, John D.; Roth, Robert H.; Sleeman, Mark W.; Picciotto, Marina R.; Tschöp, Matthias H.; Xiao-Bing Gao; Horvath, Tamas L.; Liu, Zhong-Wu; Tschöp, Matthias H; Gao, Xiao-Bing // Journal of Clinical Investigation;Dec2006, Vol. 116 Issue 12, p3229 

    The gut hormone ghrelin targets the brain to promote food intake and adiposity. The ghrelin receptor growth hormone secretagogue 1 receptor (GHSR) is present in hypothalamic centers controlling energy metabolism as well as in the ventral tegmental area (VTA), a region important for motivational...

  • Mechanisms of cardiac arrhythmias and sudden death in transgenic rabbits with long QT syndrome. Brunner, Michael; Xuwen Peng; Gong Xin Liu; Xiao-Qin Ren; Ziv, Ohad; Choi, Bum-Rak; Mathur, Rajesh; Hajjiri, Mohammed; Odening, Katja E.; Steinberg, Eric; Folco, Eduardo J.; Pringa, Ekatherini; Centracchio, Jason; Macharzina, Roland R.; Donahay, Tammy; Schofield, Lorraine; Rana, Naveed; Kirk, Malcolm; Mitchell, Gary F.; Poppas, Athena // Journal of Clinical Investigation;Jun2008, Vol. 118 Issue 6, p2246 

    Long QT syndrome (LQTS) is a heritable disease associated with ECG QT interval prolongation, ventricular tachycardia, and sudden cardiac death in young patients. Among genotyped individuals, mutations in genes encoding repolarizing K+ channels (LQT1:KCNQ1; LQT2:KCNH2) are present in...

  • A Small Fraction of Strongly Cooperative Sodium Channels Boosts Neuronal Encoding of High Frequencies. Huang, Min; Volgushev, Maxim; Wolf, Fred // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Generation of action potentials (APs) is a crucial step in neuronal information processing. Existing biophysical models for AP generation almost universally assume that individual voltage-gated sodium channels operate statistically independently, and their avalanche-like opening that underlies...

  • Integration of Sensory Quanta in Cuneate Nucleus Neurons In Vivo. Bengtsson, Fredrik; Brasselet, Romain; Johansson, Roland S.; Arleo, Angelo; Jörntell, Henrik // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Discriminative touch relies on afferent information carried to the central nervous system by action potentials (spikes) in ensembles of primary afferents bundled in peripheral nerves. These sensory quanta are first processed by the cuneate nucleus before the afferent information is transmitted...

  • Structural biology: Peering into the spark of life. Horn, Richard // Nature;7/21/2011, Vol. 475 Issue 7356, p305 

    The article focuses on the study by J. Payandeh and colleagues on the crystal structure of the voltage-gated sodium channel NavAb. It states that sodium channels of cell membranes play a critical role in the induction of bioeletrical events, which lead to hormone release or muscle contraction....

  • A Statistical Model for In Vivo Neuronal Dynamics. Surace, Simone Carlo; Pfister, Jean-Pascal // PLoS ONE;11/16/2015, Vol. 10 Issue 11, p1 

    Single neuron models have a long tradition in computational neuroscience. Detailed biophysical models such as the Hodgkin-Huxley model as well as simplified neuron models such as the class of integrate-and-fire models relate the input current to the membrane potential of the neuron. Those types...

  • Intracellular recording of action potentials by nanopillar electroporation. Xie, Chong; Lin, Ziliang; Hanson, Lindsey; Cui, Yi; Cui, Bianxiao // Nature Nanotechnology;Mar2012, Vol. 7 Issue 3, p185 

    Action potentials have a central role in the nervous system and in many cellular processes, notably those involving ion channels. The accurate measurement of action potentials requires efficient coupling between the cell membrane and the measuring electrodes. Intracellular recording methods such...

  • Recent advances in Cys-loop receptor structure and function. Sine, Steven M.; Engel, Andrew G. // Nature;3/23/2006, Vol. 440 Issue 7083, p448 

    Throughout the nervous system, moment-to-moment communication relies on postsynaptic receptors to detect neurotransmitters and change the membrane potential. For the Cys-loop superfamily of receptors, recent structural data have catalysed a leap in our understanding of the three steps of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics