TITLE

Separation of Marketed Formulation containing Hydrochlorothiazide Amlodipine and Losartan through RP-HPLC Method

AUTHOR(S)
Sharma, Rajesh; Khanna, Sunil; Mishra, Ganesh Prasad
PUB. DATE
March 2016
SOURCE
International Journal of Pharmacy & Life Sciences;Mar2016, Vol. 7 Issue 3, p4937
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An attempt has been made to develop and validate an economical procedure for simultaneous estimation of hydrochlorothiazide, amlodipine and losartan in tablet dosage form. Chromatography was performed on a 25cm×4.6 mm i.d, 5μm particle, C18 column with Mixture of (A) Acetonitrile, methanol (65:35) (B) 0.4% triethylamine in 10 mM sodium dihydrogen phosphate monohydrate (NaH2PO4.H2O) buffer of the ratio of A:B is 60:40 v/v, adjusted to pH 3.0 with o-phosphoric acid (5%v/v) was used as a mobile phase at a flow rate of 1.5 ml/min. UV detection was performed at 230 nm. Total run time was less then 10 min; retention time for hydrochlorothiazide, amlodipine and losartan were 2.191, 3.780, and 5.450 minutes respectively. Limits of detection were 0.014, 0.10 and 0.0095 ng/mL limits of quantification were 0.043, 0.329 and 0.029 ng/mL for hydrochlorothiazide, amlodipine and losartan respectively. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and without any interference by the excipients.
ACCESSION #
114795791

 

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