TITLE

Intravascular naked DNA vaccine encoding glycoprotein B induces protective humoral and cellular immunity against herpes simplex virus type 1 infection in mice

AUTHOR(S)
Cui, F-D.; Asada, H.; Kishida, T.; Itokawa, Y.; Nakaya, T.; Ueda, Y.; Yamagishi, H.; Gojo, S.; Kita, M.; Imanishi, J.; Mazda, O.
PUB. DATE
December 2003
SOURCE
Gene Therapy;Dec2003, Vol. 10 Issue 25, p2059
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Naked plasmid DNA (pDNA) vaccine expressing herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) was tested for protective activity against acute HSV-1 infection in mice. The pDNA was intravenously injected into Balb/c mice via their tail vein under high pressure, and the vaccination was performed two times at an interval of 7 days. The gB gene vaccination significantly protected the mice from subsequent intraperitoneal challenge with a lethal dose of HSV-1, which killed all the animals given control plasmid or saline. The protective activity was correlated with the dose of the plasmid inoculated, the survival rate reaching 83% in mice vaccinated with 5?µg of pDNA. The vaccinated mice were also protected from latent HSV infection. The immunized mice showed significant elevation in neutralizing antibody against HSV-1 as well as serum levels of interleukin-12 (IL-12) and interferon-? (IFN-?). When mice were immunized with 5?µg of an Epstein-Barr virus (EBV)-based plasmid vector harboring the gB, the cytotoxic T lymphocytes (CTLs) activity and proliferative response for HSV-1 were also induced. The results strongly suggest that intravenous immunization of naked pDNA may induce humoral and cellular immune responses against the virus, leading to a significant prophylactic outcome against HSV-1 infection in mice.Gene Therapy (2003) 10, 2059-2066. doi:10.1038/sj.gt.3302114
ACCESSION #
11281385

 

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