Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey

January 2016
Veterinární Medicína;2016, Vol. 61 Issue 1, p17
Academic Journal
This study was aimed at determining the presence of C. burnetii in cattle and sheep herds in the Kars region of Turkey using serological and molecular methods. As a serological technique, C.burnetii IgG in blood sera and milk samples were investigated with ELISA. The results of these examinations revealed that 108 (43.2%) out of 250 sheep blood serum samples and 52 (14.85%) out of 350 cattle blood serum samples were seropositive for C. burnetii antibodies by ELISA. Out of 350 cattle and 250 sheep milk samples examined with ELISA, 36 (10.28%) and 42 (16.8%) were found to be seropositive, respectively. For molecular analysis, a Trans-PCR amplifying the IS1111A transposase gene of C. burnetii was conducted. Five (1.42%) out of 350 cattle milk samples, and one (0.4%) from 250 sheep milk samples were found to be positive for C. burnetii DNA. The results obtained in this study have demonstrated the presence of Q fever in cattle and sheep in the Kars region, and the dissemination of the infectious agent within milk. This situation poses a potential risk for animal and human health. Ultimately, this study points the way to future investigations into the presence of C. burnetii, which causes Q fever in cattle and sheep, and will contribute to the protection and control of the disease.


Related Articles

  • Coxiella burnetii in bulk tank milk samples from dairy goat and dairy sheep farms in the Netherlands in 2008. den Brom, R. van; van Engelen, E.; Luttikholt, S.; Moll, L.; van Maanen, K.; Vellema, P. // Veterinary Record: Journal of the British Veterinary Association;3/24/2012, Vol. 170 Issue 12, p310 

    The article presents a study on the prevalence of Q fever among samples of dairy goat and sheep bulk tank milk (BTM) in the Netherlands. The study used various methods such as real-time polymerase chain reaction (RT-PCT), enzyme-linked immunosorbent assay (ELISA) and immunohistorchemistry (IHC)....

  • Spread of Coxiella burnetii infection in a flock of sheep after an episode of Q fever. Berri, M.; Crochet, D.; Santiago, S.; Rodolakis, A. // Veterinary Record: Journal of the British Veterinary Association;12/3/2005, Vol. 157 Issue 23, p737 

    The article investigates the prevalence of Coxiella burnetii infection in the sheep flock following an episode of Q fever. The ewes were treated with oxytetracycline to reduce the shedding of C. burnetii and the prevent further abortions. with this regard, the seroprevelance of C. burnetii in...

  • Immuno-PCR for the early serological diagnosis of acute infectious diseases: the Q fever paradigm. Malou, N.; Renvoise, A.; Nappez, C.; Raoult, D. // European Journal of Clinical Microbiology & Infectious Diseases;Aug2012, Vol. 31 Issue 8, p1951 

    To reduce the delay in diagnosis of Q fever, we have adapted the ultrasensitive immuno-PCR method for the detection of Phase II IgM anti- Coxiella burnetii. We compared its performance to ELISA, IFA and PCR using 31 acute Q fever sera and 50 control sera. The best sensitivity was obtained by...

  • Serological Evidence of Coxiella burnetii Infection in Cattle and Goats in Bangladesh. Haider, Najmul; Rahman, Md.; Khan, Salah; Mikolon, Andrea; Osmani, Muzaffor; Gurley, Emily; Shanta, Ireen; Paul, Suman; Macfarlane-Berry, Laura; Islam, Ariful; Islam, Ausraful; Desmond, James; Epstein, Jonathan; Priestley, Rachael; Kersh, Gilbert; Rahman, Mohammed; Daszak, Peter; Luby, Stephen; Massung, Robert; Zeidner, Nord // EcoHealth;Jun2015, Vol. 12 Issue 2, p354 

    We tested 1149 ruminant sera conveniently collected from three districts of Bangladesh to identify the serological evidence of Coxiella burnetii infection in cattle and goats by enzyme-linked immunosorbent assay. We found that 0.7% (8/1149) of ruminants had detectable immunoglobulin G for C....

  • Progression of Coxiella burnetii infection after implementing a two-year vaccination program in a naturally infected dairy cattle herd. Piñero, Alvaro; Barandika, Jesús F.; Hurtado, Ana; García-Pérez, Ana L. // Acta Veterinaria Scandinavica;2014, Vol. 56 Issue 1, p1 

    Background The high prevalence of Coxiella burnetii infection in dairy cattle herds recently reported and the long survival time of the bacterium in the environment pose a risk to human and animal health that calls for the implementation of control measures at herd level. This study presents the...

  • Comprehensive in vivo Mapping of the Human Basal Ganglia and Thalamic Connectome in Individuals Using 7T MRI. Lenglet, Christophe; Abosch, Aviva; Yacoub, Essa; De Martino, Federico; Sapiro, Guillermo; Harel, Noam // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Basal ganglia circuits are affected in neurological disorders such as Parkinson's disease (PD), essential tremor, dystonia and Tourette syndrome. Understanding the structural and functional connectivity of these circuits is critical for elucidating the mechanisms of the movement and...

  • Surveillance for Malaria Elimination in Swaziland: A National Cross-Sectional Study Using Pooled PCR and Serology. Hsiang, Michelle S.; Hwang, Jimee; Kunene, Simon; Drakeley, Chris; Kandula, Deepika; Novotny, Joseph; Parizo, Justin; Jensen, Trevor; Tong, Marcus; Kemere, Jordan; Dlamini, Sabelo; Moonen, Bruno; Angov, Evelina; Dutta, Sheetij; Ockenhouse, Christian; Dorsey, Grant; Greenhouse, Bryan // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Background: To guide malaria elimination efforts in Swaziland and other countries, accurate assessments of transmission are critical. Pooled-PCR has potential to efficiently improve sensitivity to detect infections; serology may clarify temporal and spatial trends in exposure....

  • Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia. Nyirenda, Stanley S.; Hang’ombe, Bernard M.; Kilonzo, Bukheti S.; Kabeta, Mathews N.; Cornellius, Mundia; Sinkala, Yona; Hang'ombe, Bernard M // Tropical Doctor;Jan2017, Vol. 47 Issue 1, p38 

    Plague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century. Two months after a suspected plague outbreak in Nyimba district, samples were collected from 94 livestock (goats and...

  • Comparison of ELISA and indirect immunofluorescent antibody assay detecting Coxiella burnetii IgM phase II for the diagnosis of acute Q fever. Meekelenkamp, J.; Schneeberger, P.; Wever, P.; Leenders, A. // European Journal of Clinical Microbiology & Infectious Diseases;Jun2012, Vol. 31 Issue 6, p1267 

    A commercially available enzyme-linked immunosorbent assay (ELISA) detecting Coxiella burnetii phase II-specific IgM for the diagnosis of acute Q fever was compared with indirect immunofluorescent antibody assay (IFA). IFA is the current reference method for the detection of antibodies against...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics