Antigenic distribution of Streptococcus agalactiae isolates from pregnant women at Garankuwa hospital - South Africa

Chukwu, Martina O.; Mavenyengwa, Rooyen Tinago; Monyama, Charles M.; Bolukaoto, John Y.; Lebelo, Sogolo L.; Maloba, Motlatji R. B.; Nchabeleng, Maphoshane; Moyo, Sylvester Rogers
December 2015
Germs;Dec2015, Vol. 5 Issue 4, p125
Academic Journal
Introduction Streptococcus agalactiae (group B streptococcus; GBS) is globally recognised as one of the leading causes of neonatal sepsis and meningitis. It also causes adverse pregnancy outcomes such as stillbirth and miscarriages. Incidence of invasive disease is increasing in non-pregnant adults with underlying medical conditions (e.g., diabetes mellitus). Epidemiological studies of GBS infections are based on capsular serotyping. Genotyping of the surface anchored protein genes is also becoming an important tool for GBS studies. Currently ten different GBS serotypes have been identified. This study was performed to determine the prevalence of GBS capsular types (CTs) and surface anchored protein genes in isolates from colonized pregnant women attending antenatal clinic, at Dr George Mukhari Academic Hospital, Garankuwa, Pretoria, South Africa. Methods The samples were collected over 11 months and cultured on selective media. GBS was identified using different morphological and biochemical tests. Capsular typing was done using latex agglutination test and conventional PCR. Multiplex PCR with specific primers was used to detect the surface anchored protein genes. Results Of the 413 pregnant women recruited, 128 (30.9%) were colonized with GBS. The capsular polysaccharide (CPS) typing test showed that CPS type III (29.7%) was the most prevalent capsular type followed by CPS type Ia (25.8%), II (15.6%), IV (8.6%), V (10.9%) and Ib (8.6%); 0.7% of the isolates were nontypeable. Multiplex PCR revealed that the surface proteins genes were possessed by all the capsular types: rib (44.5%), bca (24.7%), alp2/3 (17.9%), epsilon (8.6%) and alp4 (4.7%). Conclusion The common capsular types found in this study are Ia, III, and II. The most common protein genes identified were rib and bca, and the distribution of the surface protein genes among the isolates of different capsular types showed similar trends to the distribution reported from previous studies.


Related Articles

  • Correction: Vol. 21, No. 4.  // Emerging Infectious Diseases;Aug2015, Vol. 21 Issue 8, p1489 

    A correction to the article "Population Structure and Antimicrobial Resistance of Invasive Serotype IV Group B Streptococcus, Toronto, Ontario, Canada" is presented.

  • Group B Streptococci Colonization in Pregnant Women: Is Screening Necessary? Patil, K. P.; Singla, S. S.; Nagmoti, M. B.; Swamy, M. K. // Journal of South Asian Federation of Obstetrics & Gynecology;May-Aug2013, Vol. 5 Issue 2, p64 

    Objectives: Group B Streptococcus (GBS) has been recognized as the leading cause of serious neonatal infections through mother--fetal vertical transmission in the west, however, in India, its spectrum is largely under estimated. The present study was carried out to find the incidence of...

  • Pregnant or Thinking About Getting Pregnant?  // Preventing Infections in Pregnancy Fact Sheet;2011, p1 

    The article offers tips for women who are pregnant and or planning to get pregnant can prevent infection. It suggests that hands must be washed with soap and running water and meat must be cooked to avoid harmful bacteria and viruses that can be dangerous for the mother and the unborn baby. It...

  • In Vitro Resistance to Macrolides and Clindamycin by Group B Streptococcus Isolated from Pregnant and Nonpregnant Women. Lambiase, Antonietta; Agangi, Annalisa; Del Pezzo, Mariassunta; Quaglia, Filomena; Testa, Antonio; Rossano, Fabio; Martinelli, Pasquale; Catania, Maria Rosaria // Infectious Diseases in Obstetrics & Gynecology;2012, p1 

    Background. Despite the introduction of screening bases intrapartum prophylaxis, Streptococcus agalactiae is still an important etiological agent of perinatal infections. The increasing rate of resistance and the differences in resistance pattern among countries suggest that a program of...

  • IN-VITRO EVALUATION OF THE ANTAGONISTIC EFFECTS OF THE PROBIOTICS LACTOBACILLUS RHAMNOSUS HN001 AND FLORAJEN 3 AGAINST GROUP B STREPTOCOCCI. Ephraim, Eden; Schultz, Ronald D.; Duster, Megan; Warrack, Simone; Spiegel, Carol A.; Safdar, Nasia // International Journal of Probiotics & Prebiotics;Aug-Nov2012, Vol. 7 Issue 3/4, p113 

    Group B Streptococci (GBS) are among the leading causes of bacterial infectious causing serious illnesses and death, particularly in newborns. Third trimester screening of pregnant women for GBS colonization and intrapartum antibiotic treatment of those who are positive is standard of care. The...

  • The Pore-Forming Toxin β hemolysin/cytolysin Triggers p38 MAPK-Dependent IL-10 Production in Macrophages and Inhibits Innate Immunity. Bebien, Magali; Hensler, Mary E.; Davanture, Suzel; Li-Chung Hsu; Karin, Michael; Park, Jin Mo; Alexopoulou, Lena; Liu, George Y.; Nizet, Victor; Lawrence, Toby // PLoS Pathogens;Jul2012, Vol. 8 Issue 7, Special section p1 

    Group B Streptococcus (GBS) is a leading cause of invasive bacterial infections in human newborns and immunecompromised adults. The pore-forming toxin (PFT) β hemolysin/cytolysin (βh/c) is a major virulence factor for GBS, which is generally attributed to its cytolytic functions. Here we...

  • GBS Testing During Pregnancy.  // American Family Physician;7/1/2012, Vol. 86 Issue 1, Special section p1 

    The article explains the importance of group B streptococcus (GBS) testing during pregnancy. It notes the ability of GBS to live in a woman's vagina, rectum, or urine and warns that the baby can catch it and get very sick if a pregnant woman has GBS. The test is conducted by a doctor by swabbing...

  • Streptococcus agalactiae (Lancefield Group B) Bacteraemia in Nonpregnant Adults. Wang, T. K. F; Fung, A. M. Y.; Woo, P. C. Y.; Yuen, K. Y.; Wong, S. S. Y. // European Journal of Clinical Microbiology & Infectious Diseases;Feb2002, Vol. 21 Issue 2, p140 

    This article focuses on Streptococcus agalactiae which has long been a major cause of neonatal and postpartum sepsis. In the last two decades, reports have increased regarding group B streptococcal infections in nonpregnant adults with the emergence of serotype V in infants and adults. Although...

  • Preventing invasive Group B Streptococcus (GBS) disease in South African infants: Time for change. Dangor, Z.; Lala, S. G.; Madhi, S. A. // South African Journal of Child Health;Aug2015, Vol. 9 Issue 3, p69 

    The article focuses on Group B Streptococcus (GBS) infection seen among infants, adults and pregnant women in South Africa that exhibits as early-onset disease (EOD) and late-onset disease (LOD) in life of the victim of the disease. Topics discussed includes suboptimal implementation of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics