Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

November 2015
Arquivos Brasileiros de Oftalmologia;Nov/Dec2015, Vol. 78 Issue 6, p356
Academic Journal
Purpose: To evaluate the ability of real-time quantitative PCR (qPCR) for detecting Toxoplasma gondii DNA in the peripheral blood and aqueous humor of patients with toxoplasmic active focal necrotizing retinochoroiditis. Methods: Fifty-five patients with infectious uveitis seen from 2009 to 2013 at the Department of Ophthalmology and Visual Sciences of the Federal University of São Paulo were enrolled in this study. Forty-three patients had toxoplasmic active focal necrotizing retinochoroiditis, and the remaining 12 had non-toxoplasmic infectious uveitis and served as controls. qPCR analysis for T. gondii DNA was performed on the patients' peripheral blood and aqueous humor samples. Results: The qPCR was positive for T. gondii DNA in 37.21% (16/43) of the aqueous humor samples and 2.33% (1/43) of the peripheral blood samples; further, 16.27% (7/43) of the patients had positive results in both their blood and aqueous humor samples. Conclusion: qPCR was able to detect T. gondii DNA in patients with toxoplasmic active focal necrotizing retinochoroiditis in the blood as well as the aqueous humor and can help with the diagnosis of the disease.


Related Articles

  • Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR. Sang-Eun Lee; Sung-Hee Hong; Seong-Ho Lee; Young-II Jeong; Su Jin Lim; Oh Woong Kwon; Sun Hyun Kim; Young Sung You; Shin-Hyeong Cho; Won-Ja Lee // Korean Journal of Parasitology;Sep2012, Vol. 50 Issue 3, p229 

    Toxoplasma gondii is a zoonotic parasite resulting in human infections and one of the infectious pathogens leading to uveitis and retinochoroiditis. The present study was performed to assess T. gondii infection in 20 ocular patients with chronic irregular recurrent uveitis (20 aqueous humor and...

  • Unusual Abundance of Atypical Strains Associated with Human Ocular Toxoplasmosis. Grigg, Michael E.; Ganatra, Jyotsom; Boothroyd, John C.; Margolis, Tood P. // Journal of Infectious Diseases;9/1/2001, Vol. 184 Issue 5, p633 

    To facilitate genotyping of Toxoplasma gondii in vitreous fluid of patients with severe or atypical ocular toxoplasmosis, polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) assays were developed for SAG3 (p43) and SAG4 (p18), 2 single-copy surface antigen genes....

  • Clinical Features and Treatment of Ocular Toxoplasmosis. Young-Hoon Park; Ho-Woo Nam // Korean Journal of Parasitology;Aug2013, Vol. 51 Issue 4, p393 

    Ocular toxoplasmosis is a disease caused by the infection with Toxoplasma gondii through congenital or acquired routes. Once the parasite reaches the retina, it proliferates within host cells followed by rupture of the host cells and invasion into neighboring cells to make primary lesions....

  • Aqueous humor polymerase chain reaction in uveitis - utility and safety. Chronopoulos, Argyrios; Roquelaure, Daniel; Souteyrand, Georges; Seebach, Jörg Dieter; Schutz, James Scott; Thumann, Gabriele // BMC Ophthalmology;10/28/2016, Vol. 16, p1 

    Background: To study the value and safety of aqueous humor polymerase chain reaction (PCR) analysis for Herpes simplex, varicella zoster, cytomegalovirus, Epstein-Barr virus and Toxoplasma gondii in patients with uveitis. Methods: Records of 45 consecutive patients with anterior and posterior...

  • Prophylaxis for Ocular Toxoplasmosis. Kopec, R.; De Caro, G.; Chapnick, E.; Ghitan, M.; Saffra, N. // Clinical Infectious Diseases;11/15/2003, Vol. 37 Issue 10, pe147 

    Reports on the two medical cases related to toxoplasma gondii and ocular toxoplasmosis in the U.S. Pathology of the toxoplasma gondii; Characterization of ocular toxoplasmosis; Details of the medical cases involving a male and a female.

  • Seroprevalence and genetic characterization of Toxoplasma gondii in cancer patients in Anhui Province, Eastern China. Lin Wang; Liu-yuan He; De-di Meng; Zhao-wu Chen; He Wen; Gong-si Fang; Qing-li Luo; Kai-quan Huang; Ji-long Shen // Parasites & Vectors;2015, Vol. 8 Issue 1, p1 

    Background: Recent studies have indicated the predominance of Toxoplasma gondii genotype Chinese 1 in animals in China. However, little is known of the genetic features of the parasite in humans. This study aims to determine the prevalence of anti-T. gondii antibodies based on which the genetic...

  • Influence of neurotoxoplasmosis characteristics on real-time PCR sensitivity among AIDS patients in Brazil Correia, Carolina C.; Melo, Heloísa R.L.; Costa, Vláudia M.A. // Transactions of the Royal Society of Tropical Medicine & Hygiene;Jan2010, Vol. 104 Issue 1, p24 

    Summary: Cerebral toxoplasmosis among individuals with AIDS may be difficult to diagnose and needs to be differentiated from other neurological diseases. A validation study was performed on real-time PCR for detecting the B1 gene of Toxoplasma gondii in the blood and cerebrospinal fluid (CSF) of...

  • Ocular toxoplasmosis associated with scleritis. Kamath, Yogish S.; Rathinam, S. R.; Kawali, Ankush // Indian Journal of Ophthalmology;Jun2013, Vol. 61 Issue 6, p295 

    We report an atypical presentation of Toxoplasma retinochoroiditis with associated scleritis in a young and immunocompetent patient. The diagnosis was done on the basis of Polymerase chain reaction of vitreous sample, and the clinical response to specific treatment. This case highlights the...

  • OCULAR TOXOPLASMOSIS : ATYPICAL PRESENTATION. J., Azlyn Azwa; W. M., Wan Norliza; M., Ibrahim // Malaysian Journal of Medical Sciences;Jan2006 Supplement, Vol. 13, p176 

    INTRODUCTION: Toxoplasmosis is due to infestation by an obligatory intracellular protozoan parasite called Toxoplasma gondii. Ocular involvement in acquired toxoplasmosis is extremely rare. CASE REPORT: We report herein a case of atypical presentation of ocular toxoplasmosis in a young...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics