TITLE

Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

AUTHOR(S)
DOS SANTOS, FABIO FELIPE; NASCIMENTO, HELOISA; MUCCIOLI, CRISTINA; DA COSTA, DEISE FIALHO; RIZZO, LUIZ VICENTE; COMMODARO, ALESSANDRA GONÇALVES; BELFORT JR, RUBENS
PUB. DATE
November 2015
SOURCE
Arquivos Brasileiros de Oftalmologia;Nov/Dec2015, Vol. 78 Issue 6, p356
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To evaluate the ability of real-time quantitative PCR (qPCR) for detecting Toxoplasma gondii DNA in the peripheral blood and aqueous humor of patients with toxoplasmic active focal necrotizing retinochoroiditis. Methods: Fifty-five patients with infectious uveitis seen from 2009 to 2013 at the Department of Ophthalmology and Visual Sciences of the Federal University of São Paulo were enrolled in this study. Forty-three patients had toxoplasmic active focal necrotizing retinochoroiditis, and the remaining 12 had non-toxoplasmic infectious uveitis and served as controls. qPCR analysis for T. gondii DNA was performed on the patients' peripheral blood and aqueous humor samples. Results: The qPCR was positive for T. gondii DNA in 37.21% (16/43) of the aqueous humor samples and 2.33% (1/43) of the peripheral blood samples; further, 16.27% (7/43) of the patients had positive results in both their blood and aqueous humor samples. Conclusion: qPCR was able to detect T. gondii DNA in patients with toxoplasmic active focal necrotizing retinochoroiditis in the blood as well as the aqueous humor and can help with the diagnosis of the disease.
ACCESSION #
112019430

 

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