Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Ferreira, Beatriz dos Santos; Branco, Alan Trindade; de Queiroz, Karlla Salim; Filho, Goncalo Apolinario de Souza
February 2002
Protein & Peptide Letters;Feb2002, Vol. 9 Issue 1, p39
Academic Journal
A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.


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