Electrochemical Immunosensor for Carcinoembryonic Antigen Detection Based on Mo-Mn3O4/MWCNTs/Chits Nanocomposite Modified ITO Electrode

Li, Wen-Ting; Wang, Yu; Deng, Fen-Fang; Liu, Li-Li; Nan, Hai-Jun; Li, He
December 2015
NANO;Dec2015, Vol. 10 Issue 8, p-1
Academic Journal
A novel electrochemical immunosensor for determination of carcinoembryonic antigen (CEA) in human serum was fabricated by depositing Mo-Mn3O4/MWCNTs/Chits nanocomposite onto an indium-tin oxide (ITO) electrode. Mo-doped Mn3O4 (MMO) was synthesized by sol-gel method and the presence of molybdenum improved its electrochemical properties. The MMO/MWCNTs/Chits nanocomposite could accelerate the electron transfer rate and enlarge the surface area to capture a large number of Carcinoembryonic Antigen (CEA). The factors influencing the performance of the immunosensor were investigated, such as incubation time, incubation temperature and pH. Under optimal conditions, the electrochemical immunosensor could detect CEA in a linear range from 0.1ngmL to 125ngmL with a detection limit of 4.9pgmL (). In addition, it exhibited high sensitivity and acceptable stability on a promising immobilization platform for signal amplification, which could be extended to other labeled recognition systems. This electrochemical immunosensor may provide potential applications for the clinical diagnosis. In the current study, a novel electrochemical immunosensor for the determination of carcinoembryonic antigen in human serum was developed. The proposed electrochemical immunosensor has good reproducibility, good selectivity, excellent stability and accepted precision on the determination of CEA in clinical human serum samples. It may provide potential applications for the clinical diagnosis.


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