New highly fluorinated polymers: Modifiers of chromatographic and electrophoretic systems

Dzema, D.; Kartsova, L.; Emelianov, G.; Sukhomlinova, E.
November 2015
Protection of Metals & Physical Chemistry of Surfaces;Nov2015, Vol. 51 Issue 6, p1100
Academic Journal
A set of four highly fluorinated copolymers that differ from each other in the length of perfluorinated pendants to the fluorine-containing polymer backbone chain, charge, and acid-base functions of terminal groups (sulfonate, carboxylate, sulfamide, and N,N-diethylcarbamide) is obtained for the first time. Packed columns for gas-liquid chromatography (GLC) are prepared on the basis of synthesized fluoropolymers as stationary phases. Evaluation of main types of interactions between fluoropolymers and analytes of different nature and relative polarity of phases obtained is performed using McReynolds constants and Kovats retention indices. It is found that, under the conditions of capillary electrophoresis (CE) and micellar electrokinetic chromatography (MEKC), the influence of fluoropolymer as a component of supporting electrolyte on the increase in the efficiency (by two to three times) and separation selectivity of neutral analytes (steroid hormones) is due to two reasons: modification of walls of quartz capillary by the polymer and formation of a pseudostationary phase. Under the conditions of high-performance thin-layer chromatography (HPTLC) the introduction of highly fluorinated polymers into eluent composition results in the increase in the efficiency by two to four times in the determination of amino acids and water-soluble vitamins.


Related Articles

  • Vitamin Analysis by Capillary Electrophoresis. Altria, K. D. // LC-GC Europe;Jul2013, Vol. 26 Issue 7, p400 

    The article discusses the role of capillary electrophoresis (CE) for vitamin analysis. It explores several separation and analysis approaches including free solution CE (FSCE), micellar electro-kinetic capillary chromatography (MECC), and micro-emulsion electro-kinetic chromatography (MEEKC)....

  • Different methods of on-line preconcentration in the electrophoretic determination of amines, amino acids, and steroid hormones. Kartsova, L.; Sidorova, A.; Bessonova, E. // Journal of Analytical Chemistry;Jul2012, Vol. 67 Issue 7, p642 

    The scope and limitations of various types of on-line preconcentration for ionogenic and neutral analytes, i.e., catecholamines, amino acids and, steroid hormones, under the conditions of capillary zone electrophoresis and micellar electrokinetic chromatography are examined. The preconcentration...

  • Capillary electrophoretic analysis of hydroxyl radicals produced by respiring mitochondria. Donoghue, Margaret; Xu, Xin; Bernlohr, David; Arriaga, Edgar // Analytical & Bioanalytical Chemistry;Jul2013, Vol. 405 Issue 18, p6053 

    Here, we report the use of a capillary electrophoretic method with laser-induced fluorescence detection to evaluate hydroxyl radicals produced by respiring mitochondria. The probe, hydroxyphenylfluorescein (HPF), is separated from the product, fluorescein, in under 5 min with zeptomole and...

  • Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins. HANCU, GABRIEL; KELEMEN, HAJNAL; RUSU, AURA; GYÉRESI, ÁRPÁD // Journal of the Serbian Chemical Society;2013, Vol. 78 Issue 9, p1413 

    A rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim and ceftriaxon). The determination of cephalosporins was performed at pH 6.8, using...

  • Principles of Micellar Electrokinetic Capillary Chromatography Applied in Pharmaceutical Analysis. Hancu, Gabriel; Simon, Brigitta; Rusu, Aura; Mircia, Eleonora; Gyéresi, Árpád // Advanced Pharmaceutical Bulletin;Mar2013, Vol. 3 Issue 1, p1 

    Since its introduction capillary electrophoresis has shown great potential in areas where electrophoretic techniques have rarely been used before, including here the analysis of pharmaceutical substances. The large majority of pharmaceutical substances are neutral from electrophoretic point of...

  • Utilization of CESI Technology for Comprehensive Characterization of Biologics. Lakshmanan, Rajeswari // Spectroscopy;Supplement 2014, p34 

    The article presents a study that illustrates the capillary electrophoresis and electrospray ionization (CESI) mass spectroscopy (MS). It provides discussion of the application and concept of CESI the MS method. It offers overview of the discussion of the result, which suggests its usefulness as...

  • Separation and quantification of polar pesticides in well, surface, and drinking water by capillary electrophoresis. Amelin, V.; Bol'shakov, D.; Tretyakov, A. // Journal of Analytical Chemistry;Nov2012, Vol. 67 Issue 11, p904 

    The optimal conditions for the separation and quantification of pesticides of the following classes by capillary electrophoresis are found: aryloxy carboxylic acids, sym-triazines, triazinones, chloroacetamides, urea derivatives, neonicotinoids, carbamates, triazoles, dithiocarbamates,...

  • Phenolic acids identification by capillary electrophoresis in Lupinus ssp. flours for application in the foods. Hernández-Chávez, J. F.; Guemes Vera, N. // International Food Research Journal;2015, Vol. 22 Issue 5, p1740 

    The free phenols and phenolic acids are best considered, since they are usually identified together during plant analysis. Acid hydrolysis of plant tissues releases a number of ether-soluble phenolic acids, some of which are universal in their distribution. These acids are either associated with...

  • Identification and determination of antibacterial substances in drugs by capillary electrophoresis. Bol'shakov, D.; Amelin, V.; Nikeshina, T. // Journal of Analytical Chemistry;Jan2016, Vol. 71 Issue 1, p94 

    Methods of the quality control of finished dosage forms by capillary zone electrophoresis and micellar electrokinetic chromatography have been proposed. The optimum conditions for the separation and determination of different classes of antibacterial substances, penicillins, fluoroquinolones,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics