New Advancements in Sample Preparation for the Enrichment of Phosphorylated Peptides and Proteins

Rainer, Matthias; Bonn, Günther K.
August 2013
Chromatography Today;Aug/Sep2013, Vol. 6 Issue 3, p18
The development of a complete analytical method includes a number of steps from sample collection to the final reporting of results. Intermediate stages involve sample storage, sample preparation, isolation of analytes, their identification and quantification. However, sample preparation is one of the most crucial processes in bioanalytical applications. The results of the experiment depend on the condition of the starting material, thus, a proper experimental model and careful sample preparation is crucial in order to obtain significant and reliable results. In the context of protein science and proteomics research, phosphoproteomics has become an important research area in several life sciences. However, phosphorylated compounds are usually present at very low concentrations. Thus, highly sensitive and selective technologies are required for their enrichment and purification. This review provides a short overview about new developments in sample preparation for the enrichment of phosphorylated peptides and proteins.


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