TITLE

Downregulation of CD147 by chitooligosaccharide inhibits MMP-2 expression and suppresses the metastatic potential of human gastric cancer

AUTHOR(S)
ZHIGUO LUO; XIAOXIA DONG; QING KE; QIWEN DUAN; LI SHEN
PUB. DATE
July 2014
SOURCE
Oncology Letters;2014, Vol. 8 Issue 1, p361
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Metastasis is considered to be the major cause of mortality in patients with cancer, and gastric cancer is a highly metastatic cancer. In the present study, the anti-metastatic activity of chitooligosaccharide (COS) in human gastric cancer cells and its underlying mechanism were investigated. It was found that COS significantly inhibited SGC-7901 cell proliferation and metastasis in a dose-dependent manner, as observed by MTT, wound-healing and Transwell assays. Quantitative real-time polymerase chain reaction and western blot analysis indicated that COS could decrease the expression of cluster of differentiation 147 (CD147) and subsequently reduce matrix metalloproteinase-2 (MMP-2) expression. A clear dose-dependent inhibition of MMP-2 activity was also observed in SGC-7901 cells following treatment with COS in gelatin zymography experiments. Furthermore, overexpression of CD147 (when transfected with pEGFP-C1 plasmid) in SGC-7901 cells partially protected against COS-induced inhibition of MMP-2. The results of the present study demonstrated the potential of COS in suppressing gastric cancer metastasis, and that the CD147/MMP-2 pathway may be involved as the key mechanism of its anti-metastatic effect.
ACCESSION #
110397735

 

Related Articles

  • Downregulation of FBP1 Promotes Tumor Metastasis and Indicates Poor Prognosis in Gastric Cancer via Regulating Epithelial-Mesenchymal Transition. Li, Jing; Wang, Ying; Li, Qing-Guo; Xue, Jin-Jun; Wang, Zhu; Yuan, Xin; Tong, Jian-Dong; Xu, Li-Chun // PLoS ONE;12/15/2016, Vol. 11 Issue 12, p1 

    Background: Recent studies indicated that some glycolytic enzymes are complicated, multifaceted proteins rather than simple components of the glycolytic pathway. FBP1 plays a vital role in glucose metabolism, but its role in gastric cancer tumorigenesis and metastasis has not been fully...

  • Claudin-4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase-2 and -9 expression. TSANN-LONG HWANG; TZU-TSUNG CHANGCHIEN; CHEE-CHAN WANG; CHI-MING WU // Oncology Letters;2014, Vol. 8 Issue 3, p1367 

    Claudin-4 is a member of a large family of transmembrane proteins known as claudins, which are essential for the formation and maintenance of tight junctions. Our previous studies have revealed that claudin-4 proteins are overexpressed in metastatic gastric cancer. To clarify the roles of...

  • Multidrug-Resistance Related Long Non-Coding RNA Expression Profile Analysis of Gastric Cancer. Wang, Ying; Wu, Kaichun; Yang, Zhiping; Zhao, Qingchuan; Fan, Dongmei; Xu, Po; Nie, Yongzhan; Fan, Daiming // PLoS ONE;8/20/2015, Vol. 10 Issue 8, p1 

    The effect of chemotherapy of gastric cancer (GC) remains very poor because of multidrug resistance (MDR). However, the mechanisms underlying MDR of GC remains far from fully understood. The aim of this study is to illustrate the potential mechanisms of the MDR of GC at mainly the long...

  • MicroRNA-206: Effective Inhibition of Gastric Cancer Progression through the c-Met Pathway. Zheng, Zhiqiang; Yan, Dongsheng; Chen, Xiaoyan; Huang, He; Chen, Ke; Li, Guangjing; Zhou, Linglin; Zheng, Dandan; Tu, LiLi; Dong, Xiang Da // PLoS ONE;7/17/2015, Vol. 10 Issue 7, p1 

    MicroRNAs are endogenous short chain nucleotide RNAs that regulate gene function by direct binding of target mRNAs. In this study, we investigated the effects of microRNA-206 (miR-206) on the development of gastric cancer. miR-206 was first confirmed to be downregulated in gastric cancer...

  • Gene or genome? The meaning of gains/losses of chromosome. Itoh, Fumio; Imai, Kohzoh // Journal of Gastroenterology;1999, Vol. 34 Issue 1, p147 

    Editorial. Comments on a study which determined the association of numerical aberrations of chromosome 17 in primary gastric cancers with lymph node metastasis. Methodology of the study; Kinds of caretaker abnormality; Arguments on the study's conclusion.

  • Whole genome and transcriptome sequencing of matched primary and peritoneal metastatic gastric carcinoma. Zhang, J.; Huang, J. Y.; Chen, Y. N.; Yuan, F.; Zhang, H.; Yan, F. H.; Wang, M. J.; Wang, G.; Su, M.; Lu, G; Huang, Y.; Dai, H.; Ji, J.; Zhang, J. N.; Jiang, Y. N.; Chen, S. J.; Zhu, Z. G.; Yu, Y. Y. // Scientific Reports;9/4/2015, p13750 

    Gastric cancer is one of the most aggressive cancers and is the second leading cause of cancer death worldwide. Approximately 40% of global gastric cancer cases occur in China, with peritoneal metastasis being the prevalent form of recurrence and metastasis in advanced disease. Currently, there...

  • Hypoxia-Inducible lncRNA-AK058003 Promotes Gastric Cancer Metastasis by Targeting γ-Synuclein. Yafang Wang; Xiangqiang Liu; Hongbo Zhang; Li Sun; Yongan Zhou; Haifeng Jin; Hongwei Zhang; Hui Zhang; Jiaming Liu; Hao Guo; Yongzhan Nie; Kaichun Wu; Daiming Fan; Helong Zhang; Lili Liu // Neoplasia;Dec2014, Vol. 16 Issue 12, p1094 

    Hypoxia has been implicated as a crucial microenvironmental factor that induces cancer metastasis. We previously reported that hypoxia could promote gastric cancer (GC) metastasis, but the underlying mechanisms are not clear. Long noncoding RNAs (lncRNAs) have recently emerged as important...

  • OCT3/4 expression is correlated with the invasion of gastric carcinoma. NA LI; WEIQIANG WANG; BIN XU; HONGYUN GONG // Oncology Letters;2014, Vol. 8 Issue 1, p12 

    The present study aimed to evaluate the effect of OCT3/4 on the invasion and metastasis ability of gastric cancer. First, the expression level of OCT3/4 was detected in gastric cancer tissues of different tumor-node-metastasis stages. Furthermore, the correlation between the expression of OCT3/4...

  • Frequent somatic allelic inactivation of the E-cadherin gene in gastric carcinomas. Becker, Karl-Friedrich; Hofler, Heinz // JNCI: Journal of the National Cancer Institute;7/19/95, Vol. 87 Issue 14, p1082 

    Examines whether E-cadherin gene is the target of allelic inactivation in gastric cancer using intragenic polymorphisms. Advantages of using intragenic polymorphisms; Methodology; Frequent somatic allelic inactivation of the E-cadherin gene in gastric carcinomas.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics