7-difluoromethoxyl-5,4'-di-n-octyl genistein inhibits ovarian cancer stem cell characteristics through the downregulation of FOXM1

July 2014
Oncology Letters;2014, Vol. 8 Issue 1, p295
Academic Journal
7-Difluoromethoxyl-5,4'-di-n-octylgenistein (DFOG) is a novel synthetic genistein analogue that possesses anti-cancer activity in a variety of cancers, including ovarian cancer. The objective of the present study was to investigate whether DFOG inhibits the self-renewal capacity of ovarian cancer stem-like cells (OCSLCs) and to identify its potential mechanism of action. It was found that the sphere-forming cells (SFCs) of the SKOV3 cell line exhibited a self-renewal capacity and high tumorigenicity, indicating that they possessed the properties of ovarian cancer stem cells (OCSCs). It was also shown for the first time that DFOG preferentially inhibited proliferation, self-renewal capacity and expression of stem cell markers [cluster of differentiation (CD)133, CD44 and aldehyde dehydrogenase 1 (ALDH1)] in the SFCs derived from the SKOV3 cells. These effects were accompanied by the downregulation of forkhead box M1 (FOXM1) expression. Overexpression of FOXM1 rescued the DFOG-induced downregulation of FOXM1, CD133, CD44 and ALDH1 protein expression. It also inhibited the self-renewal capacity of the SFCs derived from the SKOV3 cells. Thus, DFOG appears to inhibit the characteristics of OCSLCs by downregulating FOXM1 expression.


Related Articles

  • Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells. Du, Yuanda; Feng, Jinhong; Wang, Renqing; Zhang, Haijie; Liu, Jian // PLoS ONE;Jun2015, Vol. 10 Issue 6, p1 

    In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3ʹ-O-β--glucopyranoside and...

  • Enrichment and characterization of ovarian cancer stem cells and its potential clinical application. Wenxia Wang; Zhenbo Zhang; Yin Zhao; Zeng Yuan; Xingsheng Yang; Beihua Kong; Wenxin Zheng // International Journal of Clinical & Experimental Medicine;2016, Vol. 9 Issue 10, p10128 

    The cancer stem cell (CSC) theory proposes that a minor population in tumor cells with specific features, such as self-renewal and reproducible tumor phenotype could contribute to tumor relapse and chemotherapy resistance. Several studies have convincingly documented the existence of ovarian...

  • Salinomycin inhibits prostate cancer growth and migration via induction of oxidative stress. Ketola, K; Hilvo, M; Hyötyläinen, T; Vuoristo, A; Ruskeepää, A-L; Orešič, M; Kallioniemi, O; Iljin, K // British Journal of Cancer;1/3/2012, Vol. 106 Issue 1, p99 

    Background:We have shown that a sodium ionophore monensin inhibits prostate cancer cell growth. A structurally related compound to monensin, salinomycin, was recently identified as a putative cancer stem cell inhibitor.Methods:The growth inhibitory potential of salinomycin was studied in a panel...

  • Succinate dehydrogenase subunit B inhibits the AMPK-HIF-1α pathway in human ovarian cancer in vitro. Lilan Chen; Ting Liu; Shu Zhang; Jinhua Zhou; Yunfei Wang; Wen Di // Journal of Ovarian Research;2014, Vol. 7 Issue 1, p80 

    Background Ovarian carcinoma is one of the most common gynecological cancers with high mortality rates. Numerous evidences demonstrate that cancer cells undergo metabolic abnormality during tumorigenesis in tumor microenvironment and further facilitate tumor progression. Succinate dehydrogenase...

  • Catalytically active telomerase holoenzyme is assembled in the dense fibrillar component of the nucleolus during S phase. Lee, Ji; Lee, Yang; Jeong, Sun; Khadka, Prabhat; Roth, Jürgen; Chung, In // Histochemistry & Cell Biology;Feb2014, Vol. 141 Issue 2, p137 

    The maintenance of human telomeres requires the ribonucleoprotein enzyme telomerase, which is composed of telomerase reverse transcriptase (TERT), telomerase RNA component, and several additional proteins for assembly and activity. Telomere elongation by telomerase in human cancer cells involves...

  • Functional features of cancer stem cells in melanoma cell lines. Zimmerer, Rüdiger M.; Korn, Philippe; Demougin, Philippe; Kampmann, Andreas; Kokemüller, Horst; Eckardt, André M.; Gellrich, Nils-Claudius; Tavassol, Frank // Cancer Cell International;2013, Vol. 13 Issue 1, p1 

    Background: Recent evidence suggests a subset of cells within a tumor with "stem-like" characteristics. These cells are able to transplant tumors in immunodeficient hosts. Distinct from non-malignant stem cells, cancer stem cells (CSC) show low proliferative rates, high self-renewing capacity,...

  • Study of the biological characteristics of p75 neurotrophin receptor positive tongue squamous cell carcinoma cells. Tong Dongdong; Zhang Fenghe; Yao Yao; Zhang Zhaotao; Wang Jinbing; Li Qing; Zhang Xinlian // West China Journal of Stomatology;Feb2014, Vol. 32 Issue 1, p18 

    Objective To study the biological characteristics of p75 neurotrophin receptor positive (p75NTR+) tongue squamous cell carcinoma cells which were separated by flow cytometry cell sorting. Methods To determine the biological characteristics of p75NTR+ cells which were separated from Tca-8113 and...

  • A c-Met inhibitor increases the chemosensitivity of cancer stem cells to the irinotecan in gastric carcinoma. Yashiro, M; Nishii, T; Hasegawa, T; Matsuzaki, T; Morisaki, T; Fukuoka, T; Hirakawa, K // British Journal of Cancer;11/12/2013, Vol. 109 Issue 10, p2619 

    Background:Cancer stem cells (CSCs) may be postulated mediators of the chemoresistance. This study aimed to determine an effective signal inhibitor with effects on the proliferation of CSCs in combination with anticancer drugs.Methods:We used three gastric cancer cell lines and three side...

  • Multiple receptor tyrosine kinases converge on microRNA-134 to control KRAS, STAT5B, and glioblastoma. Zhang, Y; Kim, J; Mueller, A C; Dey, B; Yang, Y; Lee, D-h; Hachmann, J; Finderle, S; Park, D M; Christensen, J; Schiff, D; Purow, B; Dutta, A; Abounader, R // Cell Death & Differentiation;May2014, Vol. 21 Issue 5, p720 

    Receptor tyrosine kinases (RTKs) are co-deregulated in a majority of glioblastoma (GBM), the most common and most deadly brain tumor. We show that the RTKs MET, EGFR, and PDGFR regulate microRNA-134 (miR-134) in GBM. We find that miR-134 is downregulated in human tumors and cancer stem cells and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics