TITLE

Development of RAPD-based SCAR Marker for Rapid Identification of Trichoderma harzianum

AUTHOR(S)
Sun Yuantian; Tang Libo; Su Zhenyu; Li Li
PUB. DATE
April 2015
SOURCE
Plant Diseases & Pests;Apr2015, Vol. 6 Issue 2, p12
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Based on previous morphological classification and RAPD analysis, we recovered and sequenced genomic DNA of a specific fragment obtained from No. 45 Trichoderma harzianum strain in RAPD amplification. A pair of specific primers was synthesized and the RAPD markers were successfidly converted into SCAR markers. The SCAR molecular marker exhibited the specificity that could differ T. harzianum from other Trichoderma strains. This pair of molecular primers was used for specific PCR amplification of 45 Trichoderma strains, and T. harzianum strains could amplify a 779 bp DNA band while other strains had no amplification products.
ACCESSION #
110337575

 

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