TITLE

Optimization of the Solid-phase Fermentation Medium of Metarhizium flavoviride by Response Surface Method

AUTHOR(S)
Li Maoye; Lin Huafeng; Li Shiguang; Pan Jing; Xu Amei; Chen Dexin
PUB. DATE
February 2015
SOURCE
Plant Diseases & Pests;Feb2015, Vol. 6 Issue 1, p35
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Rice flour, puparium of yellow mealworm, peanut shell and sunflower seed shell are made into the culture medium, and the optimal solid-phase fermentation formula for M. flavoviride is figured out. In the solid-phase fermentation, Design-Expert is applied to analyze the significance of all components on the sporu-lation, and response surface method used to optimize the fermentation medium of M. flavoviride. The results show that puparium of yellow mealworm is the most significant component for the sporulation; and the optimal medium formula for the fermentation of M. flavoviride; 0.25-g rice flour, 0.14-g yellow mealworm puparium, 1.00-g peanut shell, and0.25-g sunflower seedshell. In this optimal medium, the sporulation achieves 100.013× 108 spores/mLfungal liquid, 31.7% higher than that of the initial design. Application of the optimal formula will reduce the production cost, obtain higher yield of spores, produce no pollution, and offer technical support for the future large-scale production.
ACCESSION #
110337571

 

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