TITLE

PLASMID PROFILING AND CURING OF MULTIDRUG RESISTANT ESCHERICHIA COLI RECOVERED FROM RETAIL CHICKEN MEAT

AUTHOR(S)
Gohar, M.; Sheikh, A. A.; Anjum, A. A.; Hussain, T.; Muhammad, J.; Tabbassum, A.; Kanwal, A.; Safdar, I.
PUB. DATE
August 2015
SOURCE
JAPS: Journal of Animal & Plant Sciences;Aug2015, Vol. 25 Issue 4, p984
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Confirmed E. coli (n = 25) were analyzed for the presence of plasmids and cured using temperature and sodium dodecyl sulphate (SDS) treatments. Plasmid profiling of the isolates showed that 96 % isolates were harboring plasmids of varying size and number. The average plasmid number among the isolates was 2.28, ranging from 0 -- 5 plasmids per isolate and plasmid size varied from 100bp -- 12kb which was distributed randomly in these isolates. Statistically no significant correlation (p>0.05) was found between antibiotic resistance and plasmid profiles. Plasmid curing study showed 3.9% and 16.7% curing efficiency with that of temperature and SDS treatment, respectively. Statistical analysis revealed that resistant isolates, treated with either physical or chemical treatments produced significant results (p<0.05) of plasmid curing. Antimicrobial resistance genes might be located on plasmid and statistically no relationship was observed between antibiotic resistance and plasmid profiles.
ACCESSION #
110326121

 

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