Development of Rous sarcoma Virus-like Particles Displaying hCC49 scFv for Specific Targeted Drug Delivery to Human Colon Carcinoma Cells

Kato, Tatsuya; Yui, Megumi; Deo, Vipin; Park, Enoch
November 2015
Pharmaceutical Research;Nov2015, Vol. 32 Issue 11, p3699
Academic Journal
Purpose: Virus-like particles (VLPs) have been used as drug carriers for drug delivery systems. In this study, hCC49 single chain fragment variable (scFv)-displaying Rous sarcoma virus-like particles (RSV VLPs) were produced in silkworm larvae to be a specific carrier of an anti-cancer drug. Method: RSV VLPs displaying hCC49 scFv were created by the fusion of the transmembrane and cytoplasmic domains of hemagglutinin from influenza A (H1N1) virus and produced in silkworm larvae. The display of hCC49 scFv on the surface of RSV VLPs was confirmed by enzyme-linked immunosorbent assay using tumor-associated glycoprotein-72 (TAG-72), fluorescent microscopy, and immunoelectron microscopy. Fluorescein isothiocyanate (FITC) or doxorubicin (DOX) was incorporated into hCC49 scFv-displaying RSV VLPs by electroporation and specific targeting of these VLPs was investigated by fluorescent microscopy and cytotoxicity assay using LS174T cells. Results: FITC was delivered to LS174T human colon adenocarcinoma cells by hCC49 scFv-displaying RSV VLPs, but not by RSV VLPs. This indicated that hCC49 scFv allowed FITC-loaded RSV VLPs to be delivered to LS174T cells. DOX, which is an anti-cancer drug with intrinsic red fluorescence, was also loaded into hCC49 scFv-displaying RSV VLPs by electroporation; the DOX-loaded hCC49 scFv-displaying RSV VLPs killed LS174T cells via the specific delivery of DOX that was mediated by hCC49 scFv. HEK293 cells were alive even though in the presence of DOX-loaded hCC49 scFv-displaying RSV VLPs. Conclusion: These results showed that hCC49 scFv-displaying RSV VLPs from silkworm larvae offered specific drug delivery to colon carcinoma cells in vitro. This scFv-displaying enveloped VLP system could be applied to drug and gene delivery to other target cells.


Related Articles

  • Anticancer Metallotherapeutics in Preclinical Development. Kaluđ erović, G. N.; Paschke, R. // Current Medicinal Chemistry;Nov2011, Vol. 18 Issue 31, p4738 

    This article reviews recent studies of platinum and other metal-containing antitumor drugs in preclinical development. A short overview of the development of cisplatin-based drug generations is given. Some pioneering strategies towards unconventional drugs, such as platinum complexes with...

  • Targeting tumour cells with chemical clusters. Bradley, David // New Scientist;9/8/90, Vol. 127 Issue 1733, p39 

    Reports on U.S. scientists' development of a compound called Venus Flytrap Cluster (VFC), which is effective in treating colorectal cancer. Cage of boron and carbon atoms in VFC, which acts as a carrier for a radioactive metal ion trapped inside; Radiometal ion's ability to kill a cancer cell;...

  • Functionalized Graphene Oxide as Nanocarrier for Loading and Delivery of Ellagic Acid. Kakran, M.; Sahoo, N. G.; Bao, H.; Pan, Y.; L. Li // Current Medicinal Chemistry;Oct2011, Vol. 18 Issue 29, p4503 

    In this research, we have covalently functionalized graphene oxide (GO) with hydrophilic and biocompatible Pluronic F38 (F38), Tween 80 (T80) and maltodextrin (MD) for loading and delivery of a poorly water soluble antioxidant and anticancer drug, ellagic acid (EA). The functionalized GO showed...

  • A REVIEW ON POLYMERIC MICELLAR NANOCARRIERS. DHEMBRE, G. N.; MOON, R. S.; KSHIRSAGAR, R. V. // International Journal of Pharma & Bio Sciences;Apr-Jun2011, Vol. 2 Issue 2, pP.109 

    Micelles, self-assembling nanosized (5-100 nm) colloidal particles with a hydrophobic core and hydrophilic shell are currently successfully used as pharmaceutical carriers for waterinsoluble drugs and demonstrate a series of attractive properties as drug carriers. These polymeric miscellar...

  • Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers. Dreher, Matthew R.; Liu, Wenge; Michelich, Charles R.; Dewhirst, Mark W.; Fan Yuan; Chilkoti, Ashutosh; Yuan, Fan // JNCI: Journal of the National Cancer Institute;3/1/2006, Vol. 98 Issue 5, p335 

    Background: Delivery of anticancer therapeutic agents to solid tumors is problematic. Macromolecular drug carriers are an attractive alternative drug delivery method because they appear to target tumors and have limited toxicity in normal tissues. We investigated how molecular...

  • Fabrication of Heparinized Mesoporous Silica Nanoparticles as Multifunctional Drug Carriers. Yongxiang Zhang; Xinmin Liu; Yizhong Lu; Jincai Wang; Tingfang Dong; Xiaohong Liu // Journal of Chemistry;2013, p1 

    In this study, heparinized multifunctional mesoporous silica nanoparticles were successfully synthesized and characterized. The new material not only maintains intrinsic functions of bare magnetic and fluorescent mesoporous silica materials such as targeting, imaging, and sustained release of...

  • Sustained Release and Cytotoxicity Evaluation of Carbon Nanotube-Mediated Drug Delivery System for Betulinic Acid. Tan, Julia M.; Karthivashan, Govindarajan; Arulselvan, Palanisamy; Fakurazi, Sharida; Hussein, Mohd Zobir // Journal of Nanomaterials;2014, p1 

    Carbon nanotubes (CNTs) have been widely utilized as a novel drug carrier with promising future applications in biomedical therapies due to their distinct characteristics. In the present work, carboxylic acid-functionalized single-walled carbon nanotubes (f-SWCNTs) were used as the starting...

  • Drug Delivery: Highly Condensed Boron Cage Cluster Anions in 2D Carrier and Its Enhanced Antitumor Efficiency for Boron Neutron Capture Therapy (Adv. Funct. Mater. 27/2018). Choi, Goeun; Jeon, Ie‐Rang; Piao, Huiyan; Choy, Jin‐Ho // Advanced Functional Materials;7/1/2018, Vol. 28 Issue 27, p1 

    No abstract available.

  • Carrier-Free Microspheres of an Anti-Cancer Drug Synthesized via a Sodium Catalyst for Controlled-Release Drug Delivery. Yong Xie; Xinxin Ma; Xujie Liu; Qingming Long; Yu Wang; Youwei Yao; Qiang Cai // Materials (1996-1944);Feb2018, Vol. 11 Issue 2, p1 

    There are several challenges involved in the development of effective anti-cancer drugs, including accurate drug delivery without toxic side effects. Possible systemic toxicity and the rapid biodegradation of drug carriers are potential risks in the use of carriers for drug-delivery...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics