Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s As Drug-Releasing Surfaces

Hujaya, Sry; Engbersen, Johan; Paulusse, Jos
November 2015
Pharmaceutical Research;Nov2015, Vol. 32 Issue 11, p3732
Academic Journal
Purpose: To evaluate the potential of poly(amido amine)-based multilayered thin films in surface mediated drug release. Methods: Multilayered thin films were prepared from copolymers of phenylboronic acid-functional poly(amido amine)s and chondroitin sulfate (ChS) in the presence of Alizarin Red S (ARS) as a reporter molecule. Multilayer buildup and ARS incorporation were evaluated with UV-vis spectroscopy. Glucose responsiveness of the multilayers was investigated. Finally, cellular uptake of ARS by COS-7 cells grown on the films was assessed. Results: Multilayers based on alcohol containing polymers (ABOL-BA-PAA#ChS + ARS) displayed higher ARS incorporation than multilayers based on amine-containing polymers (DAB-BA-PAA#ChS + ARS). At physiological pH, a swift initial release of up to ~40% of the ARS content was observed during the first 12 h of incubation, followed by a much slower, gradual release of ARS. The multilayers were further evaluated by culturing COS-7 cells on top of multilayer-coated well plates. Cellular uptake of the fluorescent ARS-boronate ester was quantified through flow cytometry, and a maximum uptake of up to 30% was observed. Confocal microscopy confirmed the presence of ARS-boronate ester-containing particles in the nuclei of cells. Conclusions: The investigated multilayered thin films are effective in surface-mediated delivery of the model compound ARS. These multilayered surfaces are promising as drug-releasing delivery surface for coating stents, prostheses, and other implants. [Figure not available: see fulltext.]


Related Articles

  • Responsive Boronic Acid-Decorated (Co)polymers: From Glucose Sensors to Autonomous Drug Delivery. Vancoillie, Gertjan; Hoogenboom, Richard // Sensors (14248220);Oct2016, Vol. 16 Issue 10, p1736 

    Boronic acid-containing (co)polymers have fascinated researchers for decades, garnering attention for their unique responsiveness toward 1,2- and 1,3-diols, including saccharides and nucleotides. The applications of materials that exert this property are manifold including sensing, but also...

  • Physics of two-dimensional ferroelectric polymers. Ducharme, Stephen; Palto, S. P.; Blinov, L. M.; Fridkin, V. M. // AIP Conference Proceedings;2000, Vol. 535 Issue 1, p354 

    Unique insight into the nature of ferroelectricity is emerging from the study of Langmuir-Blodgett films of vinylidene fluoride copolymers. These films are the first truly two-dimensional ferroelectrics, with thickness-independent bulk ferroelectric properties and a separate surface phase...

  • Photokinetic Drug Delivery: Light-Enhanced Permeation in an In Vitro Eye Model. Godley, Bernard F.; Kraft, Edward R.; Giannos, Steven A.; Zhao, Zhen-Yang; Haag, Anthony M.; Wen, Julie W. // Journal of Ocular Pharmacology & Therapeutics;Dec2015, Vol. 31 Issue 10, p650 

    Purpose: To investigate light-enhanced molecular movement as a potential technology for drug delivery. To do this, we developed an in vitro eye model while representing similar concentration gradient conditions and compositions found in the eye.Methods: The eye model...

  • Self-dissolving micropile array tips for percutaneous administration of insulin. Ito, Yukako; Yamazaki, Takenao; Sugioka, Nobuyuki; Takada, Kanji // Journal of Materials Science: Materials in Medicine;Feb2010, Vol. 21 Issue 2, p835 

    Two kinds of insulin were loaded into self-dissolving micropile array tip (following tip). Fully-loaded tip (f-tip) and partially-loaded tip (p-tip) were prepared using chondroitin sulfate for the percutaneous administration of insulin. One hundred micropiles were constructed on a 1.0 × 1.0...

  • Preparation and Characterization of a Collagen-Liposome-Chondroitin Sulfate Matrix with Potential Application for Inflammatory Disorders Treatment. Craciunescu, Oana; Gaspar, Alexandra; Trif, Mihaela; Moisei, Magdalena; Oancea, Anca; Moldovan, Lucia; Zarnescu, Otilia // Journal of Nanomaterials;2014, p1 

    Smart drug delivery systems with controllable properties play an important role in targeted therapy and tissue regeneration. The aim of our study was the preparation and in vitro evaluation of a collagen (Col) matrix embedding a liposomal formulation of chondroitin sulfate (L-CS) for the...

  • Effect of Different TiO-SiO Multilayer Coatings Applied by Sol-Gel Method on Antireflective Property. lari, Najme; Ahangarani, Shahrokh; Shanaghi, Ali // Journal of Materials Engineering & Performance;Jul2015, Vol. 24 Issue 7, p2645 

    Multilayer thin films prepared using the sol-gel process have been used in many antireflection applications. In this paper, antireflective nanoscale multilayer TiO-SiO coatings were formed on both sides of the glass substrates by combining sol-gel method and dip coating techniques. The coatings...

  • Local Delivery of High-Dose Chondroitinase ABC in the Sub-Acute Stage Promotes Axonal Outgrowth and Functional Recovery after Complete Spinal Cord Transection. Cheng, Chu-Hsun; Lin, Chi-Te; Lee, Meng-Jen; Tsai, May-Jywan; Huang, Wen-Hung; Huang, Ming-Chao; Lin, Yi-Lo; Chen, Ching-Jung; Huang, Wen-Cheng; Cheng, Henrich // PLoS ONE;9/22/2015, Vol. 10 Issue 9, p1 

    Chondroitin sulfate proteoglycans (CSPGs) are glial scar-associated molecules considered axonal regeneration inhibitors and can be digested by chondroitinase ABC (ChABC) to promote axonal regeneration after spinal cord injury (SCI). We previously demonstrated that intrathecal delivery of...

  • Alkanol-Induced Micelles of a Very Hydrophilic EO-PO-EO Block Copolymer: Characterization by Spectral and Scattering Methods. Parmar, Arpan; Bharatiya, Bhavesh; Patel, Keyur; Aswal, Vinod; Bahadur, Pratap // Journal of Surfactants & Detergents;Jan2013, Vol. 16 Issue 1, p105 

    The aqueous solution behavior of a PEO-PPO-PEO block copolymer (EOPOEO), was investigated in the presence of aliphatic alkanols (C, C, C and C). The non-associated polymer chains remain extremely hydrated in water, but aggregation in the form of spherical micelles was evidenced, triggered by the...

  • Electrosyntheses and Characterizations of a New Multielectrochromic Copolymer of 1-(3-methylthiophen-2-yl)pyrene and 3,4-ethylenedioxythiophene. Guoqiang Feng; Zhong Wang; Qingxin Gao; Shuang Chen; Xiaolong Xu // International Journal of Electrochemical Science;2014, Vol. 9 Issue 10, p5820 

    1-(3-Methylthiophen-2-yl)pyrene (MTP) monomer was successfully synthesized via coupling reaction. A novel conjugated copolymer based on 1-(3-methylthiophen-2-yl)pyrene (MTP) and 3,4-ethylenedioxythiophene (EDOT) was electrochemically synthesized and characterized. Characterizations of the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics