TITLE

Effect of Nitrate, Ammonium and Urea on Growth and Pinnatoxin G Production of Vulcanodinium rugosum

AUTHOR(S)
Abadie, Eric; Kaci, Lamia; Berteaux, Tom; Hess, Philipp; Sechet, Véronique; Masseret, Estelle; Rolland, Jean Luc; Laabir, Mohamed
PUB. DATE
September 2015
SOURCE
Marine Drugs;2015, Vol. 13 Issue 9, p5642
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Vulcanodinium rugosum, a recently described dinoflagellate species producing a potent neurotoxin (pinnatoxin G), has been identified in French Mediterranean lagoons and was responsible for recurrent episodes of shellfish toxicity detected by mouse bioassay. Until now, the biology and physiology of V. rugosum have not been fully investigated. We studied the growth characteristics and toxicity of a V. rugosum strain (IFR-VRU-01), isolated in the Ingril lagoon in June 2009 (North-Western French Mediterranean Sea). It was cultivated in Enriched Natural Sea Water (ENSW) with organic (urea) and inorganic (ammonium and nitrate) nitrogen, at a temperature of 25 °C and irradiance of 100 μmol/m2·s-1. Results showed that ammonium was assimilated by cells more rapidly than nitrate and urea. V. rugosum is thus an osmotrophic species using urea. Consequently, this nitrogen form could contribute to the growth of this dinoflagellate species in the natural environment. There was no significant difference (Anova, p = 0.856) between the growth rate of V. rugosum cultivated with ammonium (0.28 ± 0.11 day-1), urea (0.26 ± 0.08 day-1) and nitrate (0.24 ± 0.01 day-1). However, the production of chlorophyll a and pinnatoxin G was significantly lower with urea as a nitrogen source (Anova, p < 0.027), suggesting that nutritional conditions prevailing at the moment of the bloom could determine the cellular toxicity of V. rugosum and therefore the toxicity measured in contaminated mollusks. The relatively low growth rate (≤0.28 day-1) and the capacity of this species to continuously produce temporary cysts could explain why cell densities of this species in the water column are typically low (≤20,000 cells/L).
ACCESSION #
110070017

 

Related Articles

  • Intraspecific Differences in Responses of Marsh Frog (Pelophylax ridibundus) Tadpoles Exposed to Environmentally Relevant and Acute Levels of Ammonium Nitrate Fertilizer. Karaoglu, Handan; Kutrup, Bilal; Ogut, Hamdi // Journal of Freshwater Ecology;Sep2010, Vol. 25 Issue 3, p353 

    Marsh frog (Pelophylax ridibundus) tadpoles from two different ponds in the same region were exposed to acute (0 to 500 mg/L) and chronic (0 to 25 mg/L) levels of ammonium nitrate to assess lethal and sublethal effects (growth, duration of larval development, occurrence and frequency of...

  • Nitrate has edge in dry conditions. Abel, Charles // Farmers Weekly;1/31/2003, Vol. 138 Issue 5, p54 

    Deals with the advantage of applying urea and ammonium nitrate in dry conditions. Weather considerations in nitrate applications; Advice for growers on nitrate applications; Amount of sulphur needed in combination with nitrate.

  • Urea-only policy puts crop margins at risk. Abel, Charles // Farmers Weekly;1/31/2003, Vol. 138 Issue 5, p5 

    Discusses dangers of using urea fertilizer only throughout the season instead of ammonium nitrate. Impact on combinable crop margins; Crop yields of farms that used urea throughout the winter season; Comparison of urea nitrates and ammonium nitrates.

  • Relative Uptake of Urea and Ammonium by Dinoflagellates or Cyanobacteria in Shrimp Mesocosms. Burford, Michele // Hydrobiologia;Oct2005, Vol. 549 Issue 1-3, p297 

    The relative role of the organic nitrogen source, urea, versus ammonium as a nitrogen source for two species of dinoflagellates was compared with one species of cyanobacteria. Experiments were conducted opportunistically in nutrient-rich marine water during blooms of 34either cyanobacteria or...

  • Bioconcentration and depuration of chlorpyrifos in the marine mollusc Mytilus edulis Hernandez, F.; Lopez, F. J.; Serrano, R.; Pena, J. B. // Archives of Environmental Contamination & Toxicology;Jul1997, Vol. 33 Issue 1, p47 

    The bioconcentration and depuration of chlorpyrifos [O,O-diethyl-O-(3,5,6-trichloro-2-pyridil) phosphorothioate] for the marine mollusc Mytilus edulis was investigated under laboratory conditions in experimental aquaria. Renewal tests at concentrations of 1 and 3.2 mg/L followed by depuration...

  • On farm this week. Richardson, David; Tucker, Mark // Farmers Weekly;6/23/2006, Vol. 144 Issue 25, p31 

    The article focuses on a debate over the use of urea and ammonium nitrate in farming in Great Britain. The issue has been discussed at the recent Cereals 2006 event in the region. Ammonium nitrate is considered as an effective form of nitrogen. The high levels of volatilization of urea...

  • Ammonium and urea removal by Spirulina platensis. Converti, A.; Scapazzoni, S.; Lodi, A.; Carvalho, J. // Journal of Industrial Microbiology & Biotechnology;Jan2006, Vol. 33 Issue 1, p8 

    Different concentrations either of ammonium chloride or urea were used in batch and fed-batch cultivations of Spirulina platensis to evaluate the possibility of substituting nitrate by cheaper reduced nitrogen sources in wastewaters biotreatment. The maximum nitrogen concentration able to...

  • Real costs revealed in Inputs Price Monitor.  // Farmers Weekly;2/11/2011, Vol. 154 Issue 6, p19 

    The article discusses the results of the Input Price Monitor of the National Farmers Union (NFU) and "Farmers Weekly" which show that British farmers paid 300 British pounds per ton for ammonium nitrate in January 2011, with granular urea costing more than December 2010 prices at 310 British...

  • Managing corn in a dry spring. McClure, Angela // Southeast Farm Press Exclusive Insight;5/11/2012, p8 

    The article provides suggestions on how to manage corn on dry weather. It suggests the application of side-dress nitrogen to lessen loss and also cause the least amount of damage to dry stressed corn. When broadcasting ammonium nitrate or urea with Agrotain, it recommends the application when...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics