Hepatic [sup 31]P MRS in rat models of chronic liver disease: assessing the extent and progression of disease

Corbin, I.R.; Buist, R.; Peeling, J.; Zhang, M.; Uhanova, J.; Minuk, G.Y.
July 2003
Gut;Jul2003, Vol. 52 Issue 7, p1046
Academic Journal
Background: Hepatic adenosine triphosphate (ATP) levels are an accurate reflection of functioning hepatic mass following surgical resections and acute liver injury. Objective: To determine whether hepatic ATP levels can serve as a non-invasive means of documenting progression of chronic liver disease to cirrhosis. Methods: In vivo phosphorus-31 magnetic resonance spectroscopy ([sup 31]P MRS) was performed in three animal models of chronic liver disease. Sixty six adult Sprague-Dawley rats were subjected to either thioacetamide, carbon tetrachloride (CCl[sub 4]), or common bile duct ligation (CBDL) to induce liver disease (n = 35, 21, and 10, respectively). Serial MRS examinations, blood samples, and liver biopsies (when appropriate) were obtained throughout and/or on completion of the study. Results: Over the course of the chronic liver disease, a progressive decrease in hepatic ATP levels was consistently observed in each model. The findings were most striking when end stage liver disease (cirrhosis) was established. The reduction in hepatic ATP levels correlated with significant changes in serum albumin concentrations (CCl[sub 4] and CBDL models) and the extent of hepatocyte loss seen histologically (all models). Conclusion: The results of this study indicate that during progression of chronic liver disease to cirrhosis, there is a progressive reduction in hepatic ATP levels. In addition, changes in hepatic ATP levels correlate with changes in liver function and histology. Thus hepatic [sup 31]P MRS provides a non-invasive means of documenting the severity and progression of parenchymal and cholestotic models of chronic liver disease in rats.


Related Articles

  • A new free radical scavenger, edaravone, ameliorates oxidative liver damage due to ischemia-reperfusion in vitro and in vivo. Abe, Tomoya; Unno, Michiaki; Takeuchi, Heigo; Kakita, Tetsuya; Katayose, Yu; Rikiyama, Toshiki; Morikawa, Takanori; Suzuki, Masanori; Matsuno, Seiki // Journal of Gastrointestinal Surgery;Jul2004, Vol. 8 Issue 5, p604 

    Ischemia-reperfusion injury causes oxidative stress producing reactive oxygen species, which is a serious problem linked to morbidity and mortality in liver surgery. We investigated the effects of edaravone, a new free radical scavenger, on liver oxidative stress in vitro and in vivo. We...

  • The AquaZoids! Rutemiller, Brent; Lowenheim, Al // Swimming World & Junior Swimmer;Jun96, Vol. 37 Issue 6, p22 

    Discusses adenosine triphosphate (ATP). Production of ATP by the aerobic energy system.

  • A P2X purinoceptor expressed by a subset of sensory neurons. Chen, Chih-Cheng; Akopian, Armen N. // Nature;10/5/1995, Vol. 377 Issue 6548, p428 

    Presents a study on the molecular cloning and characterization of the P2X3 P2X receptor family in adenosine triphosphates (ATP). Channel transcript in a subset of rat dorsal-root-ganglion sensory neurons; ATP-dependent cation flux; Selectivity of expression.

  • Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory... Lewis, C.; Neidhart, S. // Nature;10/5/1995, Vol. 377 Issue 6548, p432 

    Presents a study on the cloning of a complementary DNA P2X receptor channels from rat dorsal root ganglia that had properties dissimilar to those of sensory neurons. Expression yielding adenosine triphosphate-activated currents; Formation of channels by subunit heteropolymerization.

  • CORRECTION.  // Plastics News;7/2/2007, Vol. 19 Issue 18, p3 

    A correction to the article on the story of adenosine triphosphate (ATP) Engineered Rubber and Plastics Group in the June 25, 2007 issue.

  • SWIM TRAINING. Rabalais, Scott // Swim Magazine;May/Jun2004, Vol. 20 Issue 3, p12 

    Provides information on the recycling of adenosine triphosphate during swimming.

  • Rapid Determination of Bacteria in Pools. Lee, JiYoung; Deininger, Rolf A.; Fleece, Richard M. // Journal of Environmental Health;Jul/Aug2001, Vol. 64 Issue 1, p9 

    Abstract If a method for rapid determination of bacterial levels in swimming pools and whirlpools were available, bacterial health risks could be avoided before exposure occurred. Such a method would enable public-health authorities to make timely decisions about closing or reopening pools and...

  • A crossbridge too far. Spudich, James A.; Rock, Ronald S. // Nature Cell Biology;Jan2002, Vol. 4 Issue 1, pE8 

    Provides information on myosin V, an enzyme that transduces the chemical energy of ATP hydrolysis into mechanical movement along actin filaments. Number of family types of actin-based myosin the serve different functions; Working hypotheses for nucleotide-dependent coupling of myosin V stepping.

  • Chemomechanical coupling of the forward and backward steps of single kinesin molecules. Nishiyama, Masayoshi; Higuchi, Hideo // Nature Cell Biology;Oct2002, Vol. 4 Issue 10, p790 

    The molecular motor kinesin travels processively along a microtubule in a stepwise manner. Here we have studied the chemomechanical coupling of the hydrolysis of ATP to the mechanical work of kinesin by analysing the individual stepwise movements according to the directionality of the movements....


Read the Article


Sign out of this library

Other Topics