Study of Wards Environment and Hands of Healthcare Staff as Reservoir of Bacteria

Abedian, Zeinab; Sadighian F.; Saane-ei A.; Mirkarimi Z.
March 2015
Advances in Bioresearch;Mar2015, Vol. 6 Issue 2, p60
Academic Journal
Nosocomial infections are the effective cause of the mortality rate in hospitalized patients. Hospital environment, (equipment surfaces) and the hands of hospital staff can be cause to transfer multidrug resistant and life-threatening bacteria to the patients. This study was conducted to identify the types of aerobic bacteria in the environment and healthcare workers hands for prevention of nosocomial infections in the hospital. During 17 months, the specimens were collected from the staff hands and environment of ICU, surgery, maternity unit and emergency wards, by sterile swab. Then, they were cultured on Blood agar and EMB in the sterile situation. Isolated bacteria were identified by differential medium and biochemical tests. In this investigation, 403 samples were achieved from ward staff hands (234) and medical equipments (169) of the wards. Staphylococcus aureus was the most common isolated bacteria, 144 (49.5%)from staff hands and 24(10%)from medical equipments, respectively, from total (530 isolated bacteria). Two cases of Acinetobacter.spp from ICU medical equipments and three cases of Pseudomonas aeruginosa from medical equipment of ICU and male surgical ward were isolated. With regard to the importance of S. aureus which can be MRSA, applying appropriate infection monitoring and surveillance, using standard procedures, detecting common microorganisms in the hospital , effect on reducing nosocomial infection.


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