A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation

Luo, Shuhua; Tang, Menglin; Du, Lei; Gong, Lina; Xu, Jin; Chen, Youwen; Wang, Yabo; Lin, Ke; An, Qi
January 2015
Mediators of Inflammation;1/29/2015, Vol. 2015, p1
Academic Journal
Extracorporeal circulation (ECC) is necessary for conventional cardiac surgery and life support, but it often triggers systemic inflammation that can significantly damage tissue. Studies of ECC have been limited to large animals because of the complexity of the surgical procedures involved, which has hampered detailed understanding of ECC-induced injury. Here we describe a minimally invasive mouse model of ECC that may allow more extensive mechanistic studies. The right carotid artery and external jugular vein of anesthetized adult male C57BL/6 mice were cannulated to allow blood flow through a 1/32-inch external tube. All animals (n=20) survived 30 min ECC and subsequent 60 min observation. Blood analysis after ECC showed significant increases in levels of tumor necrosis factor α, interleukin-6, and neutrophil elastase in plasma, lung, and renal tissues, as well as increases in plasma creatinine and cystatin C and decreases in the oxygenation index. Histopathology showed that ECC induced the expected lung inflammation, which included alveolar congestion, hemorrhage, neutrophil infiltration, and alveolar wall thickening; in renal tissue, ECC induced intracytoplasmic vacuolization, acute tubular necrosis, and epithelial swelling. Our results suggest that this novel, minimally invasive mouse model can recapitulate many of the clinical features of ECC-induced systemic inflammatory response and organ injury.


Related Articles

  • Hematological changes during short-term tidal flow extracorporeal life support. Griffith, G. W.; Toomasian, J. M.; Schreiner, R. J.; Dusset, C. M.; Cook, K. E.; Osterholzer, K. R.; Merz, S. I.; Bartlett, R. H. // Perfusion;Nov2004, Vol. 19 Issue 6, p359 

    Various methods exist in the clinical practice of long-term venovenous (VV) extracorporeal life support (ECLS). Among the clinical techniques used are single venous access with a dual-lumen catheter, and cannulation of the jugular and femoral veins. Tidal flow VV ECLS uses a single-lumen...

  • Aprotinin in the management of life-threatening bleeding during extracorporeal life support. Biswas, A.K.; Lewis, L.; Sommerauer, J.F. // Perfusion;Jun2000, Vol. 15 Issue 3, p211 

    Contact with the synthetic surfaces of an extracorporeal circuit induces alterations in vascular components, derangements of the coagulation cascade and a systemic inflammatory response. Aprotinin reduces intraoperative and postoperative bleeding in adults undergoing cardiopulmonary bypass;...

  • Editorial. Taylor, K. M. // Perfusion;Jul2007, Vol. 22 Issue 4, p223 

    The article discusses various reports published within the issue, including one which highlights the celebration of the life and work of Professor Denis Melrose and another on a case report relating to extracorporeal circulation and pulmonary assistance.

  • Subject-specific blood flow simulation in the human carotid artery bifurcation. Deshpande, M. D.; Ballal, Vinay; Shankapal, S. R.; Prabhu, Vinay M. D.; Srinath, M. G. // Current Science (00113891);11/10/2009, Vol. 97 Issue 9, p1303 

    Pulsatile blood flow in the human carotid artery bifurcation has been simulated by solving the incompressible Navier-Stokes equations. Subject-specific data were obtained from two volunteers. Threedimensional vascular geometry was extracted from magnetic resonance imaging scans and periodic...

  • Systemic arterial waveform analysis and assessment of blood flow during extracorporeal circulation. Romano, Salvatore M.; Scolletta, Sabino; Olivotto, Iacopo; Biagioli, Bonizella; Gensini, Gian Franco; Chiostri, Marco; Giomarelli, Pierpaolo // Perfusion;Mar2006, Vol. 21 Issue 2, p109 

    Background: The pressure recording analytical method (PRAM) is a method for real-time beat-to-beat quantification of peripheral blood flow based on the analysis of arterial waveform morphology. Since PRAM can be implemented in any conditions of flow, whether physiological or artificial, we...

  • An ultrasound model to calculate the brain blood outflow through collateral vessels: a pilot study. Zamboni, Paolo; Sisini, Francesco; Menegatti, Erica; Taibi, Angelo; Malagoni, Anna Maria; Morovic, Sandra; Gambaccini, Mauro // BMC Neurology;2013, Vol. 13 Issue 1, p1 

    Background: The quantification of the flow returning from the head through the cervical veins and the collaterals of the internal jugular vein (IJV), is becoming of prominent interest in clinical practice. We developed a novel model to calculate the cerebral venous return, normalized to the...

  • Effects of Short-Acting Anaesthetics on Haemodynamic Function as Determined by Doppler Ultrasonography in Rabbits. KAYA, Mahir; PEKCAN, Zeynep; ŞEN, Yusuf; BOZTOK, Başak; ŞENEL, Oytun Okan; BUMİN, Ali // Kafkas Universitesi Veteriner Fakultesi Dergisi;2011, Vol. 17 Issue 5, p713 

    This study was carried out to determine the effects of short-acting anaesthetics on haemodynamic function determined by Doppler ultrasonography. Prior to anaesthesia, Doppler parameters [peak systolic blood flow velocity (psBFV), end-diastolic blood flow velocity (edBFV), minimum diastolic blood...

  • Totally endoscopic closure of an atrial septal defect using the da vinci surgical system: Report of four cases. Yujiro Kikuchi; Teruaki Ushijima; Go Watanabe; Norihiko Ishikawa; Munehisa Takata; Yoshitaka Yamamoto // Surgery Today;Feb2010, Vol. 40 Issue 2, p150 

    Abstract  This report presents four cases of totally endoscopic closure of an atrial septal defect using the da Vinci Surgical System (Intuitive Surgical, Mountain View, CA, USA). The patients were diagnosed with an ostium secundum atrial septal defect and elected to undergo minimally...

  • Differential increases in blood flow velocity in the middle cerebral artery after tourniquet deflation during sevoflurane, isoflurane or propofol anaesthesia. KADOI, Y.; KAWAUCHI, C. H.; IDE, M.; SAITO, S.; MIZUTANI, A. // Anaesthesia & Intensive Care;Jul2009, Vol. 37 Issue 4, p598 

    The article details a study which investigated the comparative effects of sevoflurane, isoflurane or propofol on cerebral blood flow velocity following tourniquet deflation during orthopaedic surgery. The study included 30 patients who are undergoing orthopedic surgery and were assigned to...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics