Kinetics of the Apoptotic Response Induced by Anti-IgM Engagement of the B Cell Receptor Is Dependent on the Density of Cell Surface Immunoglobulin M Expression

Trujillo, Miguel A.; Eberhardt, Norman L.
August 2003
DNA & Cell Biology;Aug2003, Vol. 22 Issue 8, p525
Academic Journal
The effect of B cell receptor (BCR) density on anti-BCR-induced apoptosis was assessed in Ramos cell lines, expressing low, medium, or high levels of surface IgM (sIgM[sup LO], sIgM[sup MED], sIgM[sup HI]). All cells required a 6- μg/ml threshold of anti-IgM to elicit apoptosis. Anti-IgM treatment of sIgM[sup LO] cells induced growth inhibition and limited dose-independent apoptosis. Anti-IgM treatment of sIgM[sup MED] cells induced dose-independent death with a 32-h lag. Ligation of the BCR in the sIgM[sup HI] cells induced rapid apoptosis beginning by 6 h, which was dose-dependent. Secondary crosslinking reagents did not affect apoptosis, and this effect was independent of anti-IgM concentration, time, or sIgM density. These results suggest that the response to BCR engagement strongly depends on the cell surface receptor density.


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