TITLE

Variable-ambient scanning stage for a laser scanning confocal microscope

AUTHOR(S)
Sirbuly, D.J.; Schmidt, J.P.; Mason, M.D.; Summers, M.A.; Buratto, S.K.
PUB. DATE
October 2003
SOURCE
Review of Scientific Instruments;Oct2003, Vol. 74 Issue 10, p4366
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A variable-ambient scanning stage for a laser scanning confocal microscope was designed and tested. The stage allows for facile observation of both thin films and single molecule samples under inert conditions. High precision images have been acquired up to 174 μm2 using a single piezoceramic tube. The vacuum seal is achieved by simply placing the sample side of a standard 25×25 mm glass coverslip face down onto a Buna o ring and gently clamping it in place with a Teflon cap. Tests show that the stage holds a vacuum of at least 3×10[sup -6] Torr. Fluorescence experiments performed on films of an organic dye, DiIC[sub 12], as well as single molecule experiments performed on air sensitive oligo(phenylenevinylene) molecules, effectively demonstrate the ability of the scanner to reduce photo-oxidation rates. © 2003 American Institute of Physics.
ACCESSION #
10921027

 

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