TITLE

Reference gene selection for real-time quantitative PCR analysis on ovarian cryopreservation by vitrification in mice

AUTHOR(S)
Yuanyuan, Shan; Qin, Su; Rongrong, Xu; Yujing, Gao; Chengbin, Pei; Jianjun, Ma; Yanzhou, Yang; Xiuying, Pei
PUB. DATE
August 2015
SOURCE
Journal of Assisted Reproduction & Genetics;Aug2015, Vol. 32 Issue 8, p1277
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To ensure the correct interpretation of the results of quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) from ovarian tissue cryopreserved by vitrification, it is critical to normalize expression levels to a reference gene with stable messenger RNA (mRNA) expression in the vitrified/warmed ovarian tissue. The aim of this work was to identify suitable reference genes for qRT-PCR analysis during ovarian cryopreservation by vitrification. Methods: GeNorm, NormFinder, comparative Delta-CT, and BestKeeper were used to analyze the expression and stability of the 14 reference genes GAPDH, ABL1, ACTB, CDKN1A, GPER, GUSB, HPRT1, HSP90AB1, IPO8, PPIA, RPL4, RPL30, TBP, and UPAR. Results: Our results indicated that ACTB and RPL4 were relatively stable reference genes in vitrified/warmed ovaries.
ACCESSION #
109189776

 

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