Phenolic acids identification by capillary electrophoresis in Lupinus ssp. flours for application in the foods

Hernández-Chávez, J. F.; Guemes Vera, N.
August 2015
International Food Research Journal;2015, Vol. 22 Issue 5, p1740
Academic Journal
The free phenols and phenolic acids are best considered, since they are usually identified together during plant analysis. Acid hydrolysis of plant tissues releases a number of ether-soluble phenolic acids, some of which are universal in their distribution. These acids are either associated with lignin combined as ester groups or present in the alcohol-insoluble fraction bound as simple glycosides. Universal among the angiosperms are p-hydroxybenzoic acid, protocatechuic acid, vanillic acid and syringic acid. Gentict acid is also wide spread; less common are salicylic and o-protocatechuic, two acids found characteristically in the Ericaceae. Gallic acid is found in many woody plants, bound as gallotannin, but it is a very reactive substance. By contrast with the above acids, free phenols are relatively rare in plants. Hydroquinone is probably the most widely distributed; others, such as catechol, orcinol, phloroglucinol and pyrogallol, have been reported from only a few sources. The simple phenols are included here, because their identification is important in relation to determining the structure of flavonoids. The Lupinus is one of the most complex among the legumes, and its species grow in habitats ranging from sea level to the alpine tundra. Of the almost 500 known species worldwide, twelve are found in Europe and North Africa, and over three hundred in America. Some body authors identified 111 Lupinus species in Mexico, accounting for 22% of the described Lupinus taxa, although none of these have been cultivated or domesticated for food use. Use of Lupinus seeds is limited, however, by non-nutritional factors such as tannins, alkaloids and oligosaccharides. The alkaloids in Lupinus species are quinolizidine alkaloids (QA), which have very broad biological activity; for example, they can inhibit virus multiplication, bacteria proliferation and growth in certain fungi. This Legumen is promoted by health authorities in Western countries as a means of reducing the risk of diseases such as cancer, diabetes, and coronary heart disease. The capillary gel electrophoresis (CGE) was used to identify the protein fractions in the L. montanus, L. barkeri and L. albus isolates, using polymeric-coated commercial capillaries.



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