Identification of a Single Nucleotide Polymorphism at Hinf-1 Enzyme Restriction Site of Pit-1 Gene on Indonesian Bali Cattle Population

Jakaria; Noor, R. R.
August 2015
Media Peternakan;Aug2015, Vol. 38 Issue 2, p104
Academic Journal
This study aimed to determine the Pit-1 ∣ Hinf-1 gene polymorphism in Bali cattle (Bos javanicus) as Indonesian native cattle and besides Madura, Pesisir, Aceh, and Katingan cattle breeds as a comparison. DNA samples were extracted from 488 blood samples consisting of Bali (245 heads), Madura (68 heads), Aceh (25 heads), Pesisir (100 heads) and Katingan (50 heads) cattle. The diversity of the Pit-1∣Hinf-1 gene wasere analyzed using PCR-RFLP. Whereas the nucleotide base mutations were identified by sequencing. Genotyping data were analyzed by calculating the allele frequency, observed heterozygosity (Ho) and expected heterozygosity (He) values as well as Hardry-Weinberg equilibrium test using POPGENE 1.31 program. Whereas, Tthe sequence data were analyzed by using MEGA6 program. The Pit-1∣Hinf-1 gene fragment analysis showed that Bali, Madura, Pesisir, Aceh, and Katingan cattle had high BB genotype, resulting in B allele frequency of 0.982, 0.963, 0.925, 0.960, and 0.960, respectively. Ho and He values were 0.074-0.130 and 0.036-0.139, respectively. Hardy-Weinberg equilibrium test did not significant for all breed populations, except for Aceh cattle population (P<0.05). Mutation from guanine (G) to adenine (A) was found in Pit-1 gene fragment. Therefore, Pit-1∣Hinf-1 gene fragment had low genetic diversity in Bali cattle and other breeds population.


Related Articles

  • Single nucleotide polymorphisms in five adipokine genes in dairy cattle populations. Nephawe, K. A.; Spurlock, D. M. // South African Journal of Animal Science;2007, Vol. 37 Issue 3, p154 

    Genetic diversity in candidate genes for fitness and production traits was explored in three populations of dairy cattle. The study focused on adipokines, including leptin (LEP), tumor necrosis factor alpha (TNF), interleukin-8 (IL8) and interleukin-10 (IL10) as candidate genes. The three...

  • DNA sequence polymorphisms in a panel of eight candidate bovine imprinted genes and their association with performance traits in Irish Holstein-Friesian cattle. Magee, David A.; Sikora, Klaudia M.; Berkowicz, Erik W.; Berry, Donagh P.; Howard, Dawn J.; Mullen, Michael P.; Evans, Ross D.; Spillane, Charles; MacHugh, David E. // BMC Genetics;2010, Vol. 11, p93 

    Background: Studies in mice and humans have shown that imprinted genes, whereby expression from one of the two parentally inherited alleles is attenuated or completely silenced, have a major effect on mammalian growth, metabolism and physiology. More recently, investigations in livestock species...

  • Sequence duplication at the 3′ end of BoLA-DQB genes suggests multiple allelic lineages. Russell, George C. // Immunogenetics;Nov2000, Vol. 52 Issue 1/2, p101 

    Full-length cDNAs encoding the DQB genes expressed by three BoLA class II haplotypes (DH8A, DH15B, and DH24A) were amplified by reverse-transcription polymerase chain reaction, cloned, and sequenced. The sequence data revealed that the DH8A haplotype expressed two DQB genes (DQB*1005 and...

  • Characteristics of linkage disequilibrium in North American Holsteins. Bohmanova, Jarmila; Sargolzaei, Mehdi; Schenkel, Flavio S. // BMC Genomics;2010, Vol. 11, p421 

    Background: Effectiveness of genomic selection and fine mapping is determined by the level of linkage disequilibrium (LD) across the genome. Knowledge of the range of genome-wide LD, defined as a non-random association of alleles at different loci, can provide an insight into the optimal density...

  • Simplified Colour Method for the Detection of SNPs.  // Japanese Biotechnology & Medical Technology;Mar/Apr2002, Vol. 2 Issue 1, p5 

    Focuses on the development of a simplified method to detect the presence of single nucleotide polymorphisms within genes under fluorescent microscopes, reported by a research team at Kumamoto University in Japan. Mechanism of the method; Accuracy of the process according to the research team;...

  • The impact of single nucleotide polymorphism in monomeric alpha-amylase inhibitor genes from wild emmer wheat, primarily from Israel and Golan. Ji-Rui Wang; Yu-Ming Wei; Mei Deng; Nevo, Eviatar; Ze-Hong Yan; You-Liang Zheng // BMC Evolutionary Biology;2010, Vol. 10, p170 

    Background: Various enzyme inhibitors act on key insect gut digestive hydrolases, including alpha-amylases and proteinases. Alpha-amylase inhibitors have been widely investigated for their possible use in strengthening a plant's defense against insects that are highly dependent on starch as an...

  • Cellular thermotolerance is associated with heat shock protein 70.1 genetic polymorphisms in Holstein lactating cows. Basiricò, Loredana; Morera, Patrizia; Primi, Valentina; Lacetera, Nicola; Nardone, Alessandro; Bernabucci, Umberto // Cell Stress & Chaperones;Jul2011, Vol. 16 Issue 4, p441 

    Heat shock proteins (Hsp) are known to protect cells from several stressors. Nucleotide changes in the flanking regions [5′- and 3′-untranslated region (UTR)] of Hsp gene might affect inducibility, degree of expression, or stability of Hsp70 mRNA. The present study aimed to...

  • Allelic association and disease mapping. Ennis, Sarah; Maniatis, Nikolas; Collins, Andrew // Briefings in Bioinformatics;Dec2001, Vol. 2 Issue 4, p63 

    The application of allelic association to map genes for complex traits, particularly using highdensity maps of single nucleotide polymorphisms in candidate regions, is an area of very active research. Here we present some aspects of the methodology and applications to both major gene mapping,...

  • Polymorphisms in the 5'-untranslated region of the human serotonin receptor 1B (HTR1B) gene affect gene expression. Duan, J.; Sanders, A. R.; Moien, J. E.Vander; Martinolich, L.; Mowry, B. J.; Levinson, D. F.; Crowe, R. R.; Silverman, J. M.; Gejman, P. V. // Molecular Psychiatry;2003, Vol. 8 Issue 11, p901 

    We present evidence of complex balancing regulation of HTR1B transcription by common polymorphisms in its promoter. Computational analysis of the HTR1B gene predicted that a 5' segment, spanning common DNA sequence variations, T-261G, A-161T, and -182INS/DEL-181, contained a putative functional...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics