TITLE

Reflecting microscope system with a 0.99 numerical aperture designed for three-dimensional fluorescence imaging of individual molecules at cryogenic temperatures

AUTHOR(S)
Inagawa, H.; Toratani, Y.; Motohashi, K.; Nakamura, I.; Matsushita, M.; Fujiyoshi, S.
PUB. DATE
August 2015
SOURCE
Scientific Reports;8/7/2015, p12833
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We have developed a cryogenic fluorescence microscope system, the core of which is a reflecting objective that consists of spherical and aspherical mirrors. The use of an aspherical mirror allows the reflecting objective to have a numerical aperture (NA) of up to 0.99, which is close to the maximum possible NA of 1.03 in superfluid helium. The performance of the system at a temperature of 1.7 K was tested by recording a three-dimensional fluorescence image of individual quantum dots using excitation wavelengths (λex) of 532 nm and 635 nm. At 1.7 K, the microscope worked with achromatic and nearly diffraction-limited performance. The 1/e2 radius (Γ) of the point spread function of the reflecting objective in the lateral (xy) direction was 0.212 ± 0.008 μm at λex = 532 nm and was less than 1.2 times the simulated value for a perfectly polished objective. The radius Γ in the axial (z) direction was 0.91 ± 0.04 μm at λex = 532 nm and was less than 1.4 times the simulated value of Γ. The chromatic aberrations between the two wavelengths were one order of magnitude smaller than Γ in each direction.
ACCESSION #
108797764

 

Related Articles

  • Sloshing of superfluid helium in a viscous damping matrix. Snyder, H. A. // AIP Conference Proceedings;2002, Vol. 613 Issue 1, p1319 

    Operational problems may result from sloshing of liquid cryogens on space missions. Sloshing limits the pointing accuracy of spacecraft and the acceleration level of low-g experiments. The resonant frequencies of sloshing in space are low due to the small specific surface tension of cryogens....

  • A quantum-dot refrigerator. Edwards, H.L.; Niu, Q.; de Lozanne, A.L. // Applied Physics Letters;9/27/1993, Vol. 63 Issue 13, p1815 

    Presents a cryogenic refrigeration scheme. Utilization of discrete energy levels of quantum dots; Discussion of several physical limitations of the scheme; Use of lithographic techniques in the construction of the device.

  • Cryogenic two-photon laser photolithography with SU-8. Lee, Kwan H.; Green, Alex M.; Taylor, Robert A.; Sharp, David N.; Turberfield, Andrew J.; Brossard, Frederic S. F.; Williams, David A.; Briggs, G. Andrew D. // Applied Physics Letters;4/3/2006, Vol. 88 Issue 14, p143123 

    We have shown that photolithography can be used to create alignment markers on a semiconductor substrate at cryogenic temperatures. The epoxy resist SU-8 can be exposed effectively by two-photon absorption at a temperature of 4 K. By this means a spectroscopy apparatus can be used to find the...

  • Charge-controlled magnetism in colloidal doped semiconductor nanocrystals. Ochsenbein, Stefan T.; Yong Feng; Whitaker, Kelly M.; Badaeva, Ekaterina; Liu, William K.; Xiaosong Li; Gamelin, Daniel R. // Nature Nanotechnology;Oct2009, Vol. 4 Issue 10, p681 

    Electrical control over the magnetic states of doped semiconductor nanostructures could enable new spin-based information processing technologies. To this end, extensive research has recently been devoted to examination of carrier-mediated magnetic ordering effects in substrate-supported quantum...

  • Nonexponential "blinking" kinetics of single CdSe quantum dots: A universal power law behavior. Kuno, M.; Fromm, D. P.; Fromm, D.P.; Hamann, H. F.; Hamann, H.F.; Gallagher, A.; Nesbitt, D. J.; Nesbitt, D.J. // Journal of Chemical Physics;2/15/2000, Vol. 112 Issue 7 

    Single molecule confocal microscopy is used to study fluorescence intermittency of individual ZnS overcoated CdSe quantum dots (QDs) excited at 488 nm. The confocal apparatus permits the distribution of "on" and "off" times (i.e., periods of sustained fluorescence emission and darkness) to be...

  • A planar dielectric antenna for directional single-photon emission and near-unity collection efficiency. Lee, K. G.; Chen, X. W.; Eghlidi, H.; Kukura, P.; Lettow, R.; Renn, A.; Sandoghdar, V.; Götzinger, S. // Nature Photonics;Mar2011, Vol. 5 Issue 3, p166 

    Single emitters have been considered as sources of single photons in various contexts, including cryptography, quantum computation, spectroscopy and metrology. The success of these applications will crucially rely on the efficient directional emission of photons into well-defined modes. To...

  • Individual quantum dot spectroscopy probed by a cryogenic scanning probe. Mingjiao Liu; Changyi Li; Mampazhy, Arun; Chia-Hung Yang; Ming-Jey Yang // AIP Conference Proceedings;2005, Vol. 772 Issue 1, p653 

    In this paper we report the energy spectra of single electrons confined in a single InAs quantum dot, and single holes confined in a single GaSb quantum dot. These quantum dots are fabricated by laterally reducing the diameter of resonant tunneling (or resonant interband tunneling) diodes. The...

  • Fiber-based confocal microscope for cryogenic spectroscopy. Högele, Alexander; Seidl, Stefan; Kroner, Martin; Karrai, Khaled; Schulhauser, Christian; Sqalli, Omar; Scrimgeour, Jan; Warburton, Richard J. // Review of Scientific Instruments;Feb2008, Vol. 79 Issue 2, p023709 

    We describe the design and performance of a fiber-based confocal microscope for cryogenic operation. The microscope combines positioning at low temperatures along three space coordinates of millimeter translation and nanometer precision with high stability and optical performance at the...

  • High-frequency gate manipulation of a bilayer graphene quantum dot. Dröscher, S.; Güttinger, J.; Mathis, T.; Batlogg, B.; Ihn, T.; Ensslin, K. // Applied Physics Letters;7/23/2012, Vol. 101 Issue 4, p043107 

    We report transport data obtained for a double-gated bilayer graphene quantum dot. In Coulomb blockade measurements, the gate dielectric Cytopâ„¢ is found to provide remarkable electronic stability even at cryogenic temperatures. Moreover, we demonstrate gate manipulation with square shaped...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics