TITLE

Amelotin Gene Structure and Expression during Enamel Formation in the Opossum Monodelphis domestica

AUTHOR(S)
Gasse, Barbara; Liu, Xi; Corre, Erwan; Sire, Jean-Yves
PUB. DATE
July 2015
SOURCE
PLoS ONE;7/17/2015, Vol. 10 Issue 7, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Amelotin (AMTN) is an ameloblast-secreted protein that belongs to the secretory calcium-binding phosphoprotein family, which also includes the enamel matrix proteins amelogenin, ameloblastin and enamelin. Although AMTN is supposed to play an important role in enamel formation, data were long limited to the rodents, in which it is expressed during the maturation stage. Recent comparative studies in sauropsids and amphibians revealed that (i) AMTN was expressed earlier, i.e. as soon as ameloblasts are depositing the enamel matrix, and (ii) AMTN structure was different, a change which mostly resulted from an intraexonic splicing in the large exon 8 of an ancestral mammal. The present study was performed to know whether the differences in AMTN structure and expression in rodents compared to non-mammalian tetrapods dated back to an early ancestral mammal or were acquired later in mammalian evolution. We sequenced, assembled and screened the jaw transcriptome of a neonate opossum Monodelphis domestica, a marsupial. We found two AMTN transcripts. Variant 1, representing 70.8% of AMTN transcripts, displayed the structure known in rodents, whereas variant 2 (29.2%) exhibited the nonmammalian tetrapod structure. Then, we studied AMTN expression during amelogenesis in a neonate specimen. We obtained similar data as those reported in rodents. These findings indicate that more than 180 million years ago, before the divergence of marsupials and placentals, changes occurred in AMTN function and structure. The spatiotemporal expression was delayed to the maturation stage of amelogenesis and the intraexonic splicing gave rise to isoform 1, encoded by variant 1 and lacking the RGD motif. The ancestral isoform 2, housing the RGD, was initially conserved, as demonstrated here in a marsupial, then secondarily lost in the placental lineages. These findings bring new elements towards our understanding of the non-prismatic to prismatic enamel transition that occurred at the onset of mammals.
ACCESSION #
108665146

 

Related Articles

  • An X chromosome MicroRNA Cluster in the Marsupial Species Monodelphis domestica. Devor, Eric J.; Huang, Lingyan; Wise, Amanda; Peek, Andrew S.; Samollow, Paul B. // Journal of Heredity;Sep2011, Vol. 102 Issue 5, p577 

    MicroRNAs (miRNAs) are an important class of posttranscriptional gene expression regulators. In the course of mapping novel marsupial-specific miRNAs in the genome of the gray short-tailed opossum, Monodelphis domestica, we encountered a cluster of 39 actual and potential miRNAs spanning 102 kb...

  • Expression and Cellular Distribution of Ubiquitin in Response to Injury in the Developing Spinal Cord of Monodelphis domestica. Noor, Natassya M.; Møllgård, Kjeld; Wheaton, Benjamin J.; Steer, David L.; Truettner, Jessie S.; Dziegielewska, Katarzyna M.; Dietrich, W. Dalton; Smith, A. Ian; Saunders, Norman R. // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to...

  • Understanding strain-induced collagen matrix development in engineered cardiovascular tissues from gene expression profiles. Geemen, Daphne; Driessen-Mol, Anita; Baaijens, Frank; Bouten, Carlijn // Cell & Tissue Research;Jun2013, Vol. 352 Issue 3, p727 

    Mechanical conditioning is often used to enhance collagen synthesis, remodeling and maturation and, hence, the structural and mechanical properties of engineered cardiovascular tissues. Intermittent straining, i.e., alternating periods of cyclic and static strain, has previously been shown to...

  • Mef2c Regulates Transcription of the Extracellular Matrix Protein Cartilage Link Protein 1 in the Developing Murine Heart. Lockhart, Marie M.; Wirrig, Elaine E.; Phelps, Aimee L.; Ghatnekar, Angela V.; Barth, Jeremy L.; Norris, Russell A.; Wessels, Andy // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Cartilage Link Protein 1 (Crtl1) is an extracellular matrix (ECM) protein that stabilizes the interaction between hyaluronan and versican and is expressed in endocardial and endocardially-derived cells in the developing heart, including cells in the atrioventricular (AV) and outflow tract (OFT)...

  • Ameloblastin Inhibits Cranial Suture Closure by Modulating Msx2 Expression and Proliferation. Atsawasuwan, Phimon; Lu, Xuanyu; Ito, Yoshihiro; Zhang, Youbin; Evans, Carla A.; Luan, Xianghong // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Deformities of cranial sutures such as craniosynostosis and enlarged parietal foramina greatly impact human development and quality of life. Here we have examined the role of the extracellular matrix protein ameloblastin (Ambn), a recent addition to the family of non-collagenous extracellular...

  • Comparative biochemical characterization and in silico analysis of novel lipases Lip11 and Lip12 with Lip2 from Yarrowia lipolytica. Kumari, Arti; Verma, Ved; Gupta, Rani // World Journal of Microbiology & Biotechnology;Nov2012, Vol. 28 Issue 11, p3103 

    Novel lipases lip11 and lip12 from Yarrowia lipolytica MSR80 were cloned and expressed in E. coli HB101 pEZZ18 system along with lip2. These enzymes were constitutively expressed as extracellular proteins with IgG tag. The enzymes were purified by affinity chromatography and analyzed by SDS-PAGE...

  • Overexpression of CD97 Confers an Invasive Phenotype in Glioblastoma Cells and Is Associated with Decreased Survival of Glioblastoma Patients. Safaee, Michael; Clark, Aaron J.; Oh, Michael C.; Ivan, Michael E.; Bloch, Orin; Kaur, Gurvinder; Sun, Matthew Z.; Kim, Joseph M.; Oh, Taemin; Berger, Mitchel S.; Parsa, Andrew T. // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Mechanisms of invasion in glioblastoma (GBM) relate to differential expression of proteins conferring increased motility and penetration of the extracellular matrix. CD97 is a member of the epidermal growth factor seven-span transmembrane family of adhesion G-protein coupled receptors. These...

  • Developmental variations among Panagrolaimid nematodes indicate developmental system drift within a small taxonomic unit. Schiffer, Philipp; Nsah, Ndifon; Grotehusmann, Henny; Kroiher, Michael; Loer, Curtis; Schierenberg, Einhard // Development Genes & Evolution;Jun2014, Vol. 224 Issue 3, p183 

    Comparative studies of nematode embryogenesis among different clades revealed considerable variations. However, to what extent developmental differences exist between closely related species has mostly remained nebulous. Here, we explore the correlation between phylogenetic neighborhood and...

  • Age-related Changes in the Mechanical Properties of Human Skin. Daly, Colin H.; Odland, George F. // Journal of Investigative Dermatology;Jul79, Vol. 73 Issue 1, p84 

    The mechanical properties of skin have been studied both in vivo and in vitro by a variety of test methods. These properties are well matched to the function of the skin, and they depend on the geometry of the collagen and elastin networks of the dermis. The time dependence of...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics