Low-intensity two-dimensional imaging of fluorescence lifetimes in living cells

Emiliani, V.; Sanvitto, D.; Tramier, M.; Piolot, T.; Petrasek, Z.; Kemnitz, K.; Durieux, C.; Coppey-Moisan, M.
September 2003
Applied Physics Letters;9/22/2003, Vol. 83 Issue 12, p2471
Academic Journal
The use of a time- and space-correlated single-photon counting detector enables us to perform fluorescence lifetime imaging microscopy in living cells with a temporal resolution of less than 100 ps and a spatial resolution of 500 nm. Two-dimensional (2D) maps of the fluorescence lifetimes and the corresponding prefactors are extracted by the use of a fitting program based on the Levenberg–Marquardt algorithm (Globals Unlimited). We applied this technique to extract 2D maps of protein localization in multilabeled living cells and to study protein–protein interaction by fluorescence resonance energy transfer. © 2003 American Institute of Physics.


Related Articles

  • Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy. Macháň, Radek; Kapusta, Peter; Hof, Martin // Analytical & Bioanalytical Chemistry;Aug2014, Vol. 406 Issue 20, p4797 

    We review the principles and applications of statistical filtering in multichannel fluorescence microscopy. This alternative approach to separation of signals from individual fluorophore populations has many important advantages, especially when spectral and/or temporal overlap, or the...

  • Cooperative singlet and triplet exciton transport in tetracene crystals visualized by ultrafast microscopy. Wan, Yan; Guo, Zhi; Zhu, Tong; Yan, Suxia; Johnson, Justin; Huang, Libai // Nature Chemistry;Oct2015, Vol. 7 Issue 10, p785 

    Singlet fission presents an attractive solution to overcome the Shockley-Queisser limit by generating two triplet excitons from one singlet exciton. However, although triplet excitons are long-lived, their transport occurs through a Dexter transfer, making them slower than singlet excitons,...

  • Quantum pathways for resonance energy transfer. Jenkins, Robert D.; Daniels, Gareth J.; Andrews, David L. // Journal of Chemical Physics;6/22/2004, Vol. 120 Issue 24, p11442 

    A quantum electrodynamical calculation is presented that focuses individually on the two quantum pathways or time orderings for resonance energy transfer. Conventional mathematical procedures necessitate summing the quantum pathway amplitudes at an early stage in the calculations. Here it is...

  • Optomechanics in an ultrahigh-Q two-dimensional photonic crystal cavity. Safavi-Naeini, Amir H.; Alegre, Thiago P. Mayer; Winger, Martin; Painter, Oskar // Applied Physics Letters;11/1/2010, Vol. 97 Issue 18, p181106 

    We demonstrate an ultrahigh-Q slotted two-dimensional photonic crystal cavity capable of obtaining strong interaction between the internal light field and the mechanical motion of the slotted structure. The measured optical quality factor is Q=1.2×106 for a cavity with an effective modal...

  • Ultrafast Coherent Control of Spin Precession Motion by Terahertz Magnetic Pulses. Nakajima, M.; Yamaguchi, K.; Suemoto, T. // Acta Physica Polonica, A.;Feb2012, Vol. 121 Issue 2, p343 

    We demonstrated coherent control of spin precession motion due to the ferromagnetic resonance induced by magnetic field component of ultrashort terahertz pulses. The amplitude of the precession can be controlled by the pulse separation time of the double pulse excitation technique. We succeeded...

  • Towards the Fluorescence Resonance Energy Transfer (FRET) Scanning Near-Field Optical Microscopy: Investigation of Nanolocal FRET Processes and FRET Probe Microscope. Sekatskiı, S. K.; Shubeita, G. T.; Chergui, M.; Dietler, G.; Mironov, B. N.; Lapshin, D. A.; Letokhov, V. S. // Journal of Experimental & Theoretical Physics;May2000, Vol. 90 Issue 5, p769 

    The fluorescence resonance energy-transfer (FRET) process is investigated between donor dye molecules deposited on the sample surface and acceptor dye molecules deposited on the tips of scanning nearfield and atomic force microscopes. The FRET process was observed only when the tip acquired...

  • Advanced Fluorescence Microscopy Techniques--FRAP, FLIP, FLAP, FRET and FLIM. Ishikawa-Ankerhold, Hellen C.; Ankerhold, Richard; Drummen, Gregor P. C. // Molecules;Apr2012, Vol. 17 Issue 4, p4047 

    Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric...

  • Immunological synapses: Increasing T-cell sensitivity with CD8. Minton, Kirsty // Nature Reviews Immunology;Aug2005, Vol. 5 Issue 8, p586 

    Discusses research being done on T-cell sensitivity. Reference to a study by P. P. Yachi and colleagues published in a 2005 issue of "Nature Immunology" journal; Use of microscopic evaluation of fluorescence resonance energy transfer in the study; T-cell response to ovalbumin peptide.

  • Resonant vibrational energy transfer in ice Ih. Shi, L.; Li, F.; Skinner, J. L. // Journal of Chemical Physics;6/28/2014, Vol. 140 Issue 24, p244503-1 

    Fascinating anisotropy decay experiments have recently been performed on H2O ice Ih by Timmer and Bakker [R. L. A. Timmer, and H. J. Bakker, J. Phys. Chem. A 114, 4148 (2010)]. The very fast decay (on the order of 100 fs) is indicative of resonant energy transfer between OH stretches on...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics