TITLE

IN VIVO MODELS COMBINATION FOR B16 MURINE MELANOMA STUDY

AUTHOR(S)
Pirici, D.; Mîndrilă, I.; Mîndrilă, B.; Meşină, M.; Buteică, Alice Sandra
PUB. DATE
April 2014
SOURCE
Romanian Journal of Functional & Clinical, Macro- & Microscopica;2014, Vol. 13 Issue 2, p143
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Several melanoma models have been developed on mice in the recent decades to study the tumoral line selection, cells metastatic potential and cancer immunology in order to develop new therapeutic agents. These models should allow the recreation of the tumor features in the proper microenvironmental context (tumor growth, progression, migration, invasion), should be easy to use, inexpensive, reliable and also should provide an ethically acceptable approach. The chick embryo chorioallantoic membrane (CAM) assay is an attractive and short-time in vivo model for studying cancer, tumoral cells dissemination and drug delivery systems. As the murine model is rather a time-consuming assay, we consider that the therapeutic agents discovery or/and development studies can be improved by using a combination of the CAM and murine models. A B16F10 cell line was used to create an syngeneic murine melanoma model on C57BL/6 mice. Four weeks murine melanoma tissues were harvested by fine needle aspiration and then transplantated on 8-day old chick CAMs. Analysis of the chick CAMs inoculated with mouse melanoma tissues reveal its ability to sustain murine melanoma xenografts with its morphological and tumor progression features. Our study confirmed the validity of the CAM and murine models combination that, by using the CAM as the pre-screening model should lead to the involvement of significantly fewer animals and should provide a ethically acceptable approach for therapeutic agents discovery or development studies.
ACCESSION #
108435370

 

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