TITLE

Monocyte chemoattractant protein 1 (MCP-1) released from Helicobacter pylori stimulated gastric epithelial cells induces cyclooxygenase 2 expression and activation in T cells

AUTHOR(S)
Futagami S; Hiratsuka T; Tatsuguchi A; Suzuki K; Kusunoki M; Shinji Y; Shinoki K; Iizumi T; Akamatsu T; Nishigaki H; Wada K; Miyake K; Gudis K; Tsukui T; Sakamoto C
PUB. DATE
September 2003
SOURCE
Gut;Sep2003, Vol. 52 Issue 9, p1257
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
BACKGROUND: and aims: To clarify the interaction between gastric epithelial and mucosal T cells, we examined the role of cytokines released from epithelial cells in response to Helicobacter pylori water extract protein (HPWEP) in regulating T cell cyclooxygenase 2 (COX-2) expression and activation. METHODS: Media from MKN-28 cells incubated with HPWEP for 48 hours were added to Jurkat T cells and human peripheral T cells. C-C and CXC chemokine concentrations in MKN-28 cell media, and COX-2 expression, interferon gamma (IFN-gamma), and interleukin (IL)-4 secretions in T cells were determined by western blot analysis and ELISA methods. Distributions of COX-2 positive T cells and monocyte chemoattractant protein 1 (MCP-1) in tissue specimens with H pylori associated gastritis were determined as single or double labelling by immunohistochemistry. RESULTS: MCP-1, IL-7, IL-8, and RANTES were detected in media from MKN-28 cells incubated with HPWEP. Media as a whole, and MCP-1 alone, stimulated COX-2 expression and peripheral T cell proliferation. Anti-MCP-1 antibody inhibited media stimulated COX-2 mRNA expression in Jurkat T cells. Media stimulated IFN-gamma but not IL-4 secretion from peripheral T cells, while MCP-1 stimulated IL-4 but not IFN-gamma secretion. Both stimulated cytokine release, and peripheral T cell proliferation was partially inhibited by NS-398, a specific COX-2 inhibitor. In mucosa with gastritis, COX-2 was expressed in T cells and MCP-1 was localised mainly in epithelial and mononuclear cells. MCP-1 levels and the intensity of COX-2 expression in tissue samples were closely related. CONCLUSIONS: Cytokines such as MCP-1, released from gastric epithelial cells in response to HPWEP, seem to modulate T cell immune responses, at least in part via COX-2 expression.
ACCESSION #
10631319

 

Related Articles

  • Immune restoration in head and neck cancer patients after in vivo COX-2 inhibition. Lang, Stephan; Tiwari, Sanjay; Andratschke, Michaela; Loehr, Iren; Lauffer, Lina; Bergmann, Christoph; Mack, Brigitte; Lebeau, Annette; Moosmann, Andreas; Whiteside, Theresa L.; Zeidler, Reinhard // Cancer Immunology, Immunotherapy;Oct2007, Vol. 56 Issue 10, p1645 

    To determine the immunomodulatory effects of in vivo COX-2 inhibition on leukocyte infiltration and function in patients with head and neck cancer. Patients with squamous cell carcinoma of the head and neck preoperatively received a specific COX-2 inhibitor (rofecoxib, 25 mg daily) orally for 3...

  • Cyclooxygenase (COX)-2 and Granulosa Cell Apoptosis In Vitro. Caffrey, Angela S.; Patton, William C.; Corselli, Johannah U.; Swensen, Ron E.; King, Alan; Chan, Philip J. // Journal of Assisted Reproduction & Genetics;Dec2002, Vol. 19 Issue 12, p577 

    Assesses the sensitization of c-myc on granulosa cell apoptosis. Inhibition of cyclooxygenase (COX)- 2 to c-myc; Decrease in cell viability due to COX-2 and COX-1 inhibition; Rate of cell viability in cultured granulosa cells.

  • COX-2: promoting tolerance. Morteau, Olivier // Nature Medicine;Aug99, Vol. 5 Issue 8, p867 

    Transgenic mouse studies designed to explore T cell-mediated immunity in the gut (page 900) uncover another function for COX-2.

  • Molecular Mechanisms of High Glucose-Induced Cyclooxygenase-2 Expression in Monocytes. Shanmugam, Narkunaraja; Gonzalo, Irene T. Gaw; Natarajan, Rama // Diabetes;Mar2004, Vol. 53 Issue 3, p795 

    The cyclooxygenase (COX)-2 enzyme has been implicated in the pathogenesis of several inflammatory diseases. However, its role in diabetic vascular disease is unclear. In this study, we evaluated the hypothesis that diabetic conditions can induce COX-2 in monocytes. High glucose treatment of...

  • Cyclooxygenase-2. Yumeng Mao; Poschke, Isabel; Kiessling, Rolf // OncoImmunology;Aug2013, Vol. 2 Issue 8, p1 

    Cyclooxygenase-2 and its product PGE2 play essential roles in inducing and maintaining the suppressive functions of MDSCs in cancer. Blockade of the COX-2/PGE2 pathway abolishes the induction of MDSCs in response to tumor-derived factors and thus may provide new opportunities for targeting MDSCs...

  • Macrophages induce COX-2 expression in breast cancer cells: role of IL-1β autoamplification. Hou, Zhe; Falcone, Domenick J.; Subbaramaiah, Kotha; Dannenberg, Andrew J. // Carcinogenesis;May2011, Vol. 32 Issue 5, p695 

    Tumor-associated macrophages and high levels of cyclooxygenase-2 (COX-2) are associated with poor prognosis in breast cancer patients, but their potential interdependence has not been evaluated. The objective of this study was to determine whether macrophages regulate COX-2 expression in breast...

  • The retinal pigment epithelium (RPE) induces FasL and reduces iNOS and Cox2 in primary monocytes. Hettich, Christin; Wilker, Sebastian; Mentlein, Rolf; Lucius, Ralph; Roider, Johann; Klettner, Alexa // Graefe's Archive of Clinical & Experimental Ophthalmology;Nov2014, Vol. 252 Issue 11, p1747 

    Purpose: Retinal pigment epithelium (RPE) cells may alter the phenotype of monocytes by soluble factors that may be influenced by stimulation of the RPE. Since RPE cells carry the toll-like receptor-3 (TLR3) that detects and reacts to viral infection through binding of dsRNA we investigated the...

  • CXCL10 and CCL2 mRNA expression in monocytes is inversely correlated with the HLA-DR lower fraction of monocytes in patients with renal cell carcinoma. TAKANOBU MOTOSHIMA; YOSHIHIRO KOMOHARA; HASITA HORLAD; HIROTAKE TSUKAMOTO; MITSUGU FUJITA; YOICHI SAITO; KENICHIRO TANOUE; YUTAKA KASEJIMA; YUTAKA SUGIYAMA; YOSHIAKI KAWANO; YASUHARU NISHIMURA; MOTOHIRO TAKEYA; MASATOSHI ETO // Oncology Letters;Mar2016, Vol. 11 Issue 3, p1911 

    Circulating cluster of differentiation (CD)14+ human leukocyte antigen (HLA)-DRlow/- monocytes, those with a lower HLA-DR expression or are negative for HLA-DR, are considered to be involved in systemic immunosuppression in patients with several malignant tumors. However, few studies have...

  • Monocyte chemoattractant protein 1 and macrophage cyclooxygenase 2 expression in colonic adenoma. Tanaka, S.; Tatsuguchi, A.; Futagami, S.; Gudis, K.; Wada, K.; Seo, T.; Mitsui, K.; Yonezawa, M.; Nagata, K.; Fujimori, S.; Tsukui, T.; Kishida, T.; Sakamoto, C. // Gut;Jan2006, Vol. 55 Issue 1, p54 

    Background and aims: Cyclooxygenase 2(COX-2) expression in subepithelial macrophages of colorectal adenoma has been suggested as the first in a series of steps leading to colorectal tumorigenesis. We tested the hypothesis that chemokines released from human colorectal adenoma epithelium might be...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics