Detection and Quantification of Mycoplasma genitalium in Male Patients with Urethritis

Dupin, Nicolas; Bijaoui, Gérard; Schwarzinger, Michaë; Ernault, Pauline; Gerhardt, Philippe; Jdid, Randa; Hilba, Salim; Pantoja, Christina; Buffet, Marc; Escande, Jean-Paul; Cost, Jean-Marc
August 2003
Clinical Infectious Diseases;8/15/2003, Vol. 37 Issue 4, p602
Academic Journal
Detection and quantification of Mycoplasma genitalium were evaluated in 83 patients with urethritis (group 1), 60 patients with urethral symptoms but no urethritis (group 2), and 50 asymptomatic men (group 3). Quantification of M. genitalium was carried out using real-time polymerase chain reaction (PCR) analysis of first-pass urine samples. The rate of detection of M. genitalium was significantly higher in group 1 than in groups 2 and 3 (P < .0001). The mean observed concentration of M. genitalium was 1.2 × 10[sup 4] equivalent genomes/mL of urine (range, 50 to 8 × 10[sup 4] equivalent genomes/mL). Analysis of M. genitalium load in serial urine samples collected before and after the administration of antibacterial treatment showed an association between clinical and microbiological responses, with a shift to negative PCR results in symptom-free patients. Our results illustrate the usefulness of monitoring the M. genitalium load in evaluating the susceptibility of M. genitalium to antibacterial treatment.


Related Articles

  • Mycoplasma genitalium Infections in Asymptomatic Men and Men with Urethritis Attending a Sexually Transmitted Diseases Clinic in New Orleans. Mena, Leandro; Xiaofei Wang; Mroczkowski, Tomasz F.; Martin, David H. // Clinical Infectious Diseases;11/15/2002, Vol. 35 Issue 10, p1167 

    We report the results of a study of Mycoplasma genitalium (detected with a modified polymerase chain reaction [PCR] assay) in men with urethritis and in asymptomatic control subjects at a sexually transmitted diseases clinic in New Orleans. Data for 97 men with urethritis and 184 asymptomatic...

  • Association of Mycoplasma genitalium with balanoposthitis in men with non-gonococcal urethritis. Horner, Patrick J.; Taylor-Robinson, David // Sexually Transmitted Infections;Feb2011, Vol. 87 Issue 1, p38 

    Objective To determine whether Mycoplasma genitalium is associated with balanitis and/or posthitis in a previous study of the role of M genitalium in men with acute non-gonococcal urethritis (NGU). Methods In a previous study of men with acute NGU, the existence of balanitis and/or posthitis was...

  • Expanding the Spectrum of Pathogens in Urethritis: Implications for Presumptive Therapy? Manhart, Lisa E.; Golden, Matthew R.; Marrazzo, Jeanne M. // Clinical Infectious Diseases;10/1/2007, Vol. 45 Issue 7, p872 

    The article presents the authors' comment on the article "The role of Mycoplasma genitalium and Ureaplasma urealyticum biovar 2 in postgonococcal urethritis," by S. Yokoi and colleagues, published in this journal. The authors discuss the clinical implications of the findings related to the role...

  • Mycoplasma genitalium is associated with symptomatic urethritis. Leung, A.; Eastick, K.; Haddon, L. E.; Horn, C. K.; Ahuja, D.; Horner, P. J. // International Journal of STD & AIDS;May2006, Vol. 17 Issue 5, p285 

    In order to further investigate the epidemiology of Mycoplasma genitalium, 680 men attending departments of genitourinary medicine in Bristol, Bath and Truro were studied. M. genitalium was detected in 36 men (5.3%) and was present at all three clinics. Clinically, both urethritis and the...

  • Prevalence of Mycoplasma genitalium among female students in vocational schools in Japan. Hamasuna, R.; Imai, H.; Tsukino, H.; Jensen, J. S.; Osada, Y. // Sexually Transmitted Infections;Aug2008, Vol. 84 Issue 4, p303 

    Objective: In Japan it was reported that about 9% of sexually active female teenagers had Chlamydia trachomatis. Most of them were asymptomatic, which may lead to continuing spread of the infection. Like C trachomatis, Mycoplasma genitalium is a pathogen in male non-gonococcal urethritis....

  • Treatment efficacy, treatment failures and selection of macrolide resistance in patients with high load of Mycoplasma genitalium during treatment of male urethritis with josamycin. Guschin, Alexander; Ryzhikh, Pavel; Rumyantseva, Tatiana; Gomberg, Mikhail; Unemo, Magnus // BMC Infectious Diseases;2015, Vol. 15 Issue 1, p1 

    Background: Azithromycin has been widely used for Mycoplasma genitalium treatment internationally. However, the eradication efficacy has substantially declined recent decade. In Russia, josamycin (another macrolide) is the recommended first-line treatment for M. genitalium infections, however,...

  • Azithromycin Failure in Mycoplasma genitalium Urethritis. Bradshaw, Catriona S.; Jensen, Jorgen S.; Tabrizi, Sepehr N.; Read, Timothy R. H.; Garland, Suzanne M.; Hopkins, Carol A.; Moss, Lorna M.; Fairley, Christopher K. // Emerging Infectious Diseases;Jul2006, Vol. 12 Issue 7, p1149 

    We report significant failure rates (28%, 95% confidence interval 15%-45%) after administering 1 g azithromycin to men with Mycoplasma genitalium-positive nongonococcal urethritis. In vitro evidence supported reduced susceptibility of M. genitalium to macrolides. Moxifloxacin administration...

  • The association of Chlamydia trachomatis and Mycoplasma genitalium with non-gonococcal urethritis: observations on heterosexual men and their female partners. Keane, F. E. A.; Thomas, B. J.; Gilroy, C. B.; Renton, A.; Taylor-Robinson, D.; Keane, F E // International Journal of STD & AIDS;Jul2000, Vol. 11 Issue 7, p435 

    Our objectives were to study the distribution of Chlamydia trachomatis and Mycoplasma genitalium in men with or without non-gonococcal urethritis (NGU) and their respective female partners. A case-control study was carried out to which men with or without NGU and their female partners were...

  • Effect of gatifloxacin against Mycoplasma genitalium-related urethritis: an open clinical trial.  // Sexually Transmitted Infections;Aug2011, Vol. 87 Issue 5, p389 

    The article presents a study which investigated the efficacy of gatifloxacin against Mycoplasma genitalium-related urethritis. Microbiological cure rates of gatifloxacin against C trachomatis and M genitalium were found to be 100 percent and 83 percent, respectively. The total clinical cure rate...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics