TITLE

Mapping the SRY gene in Microtus cabrerae: a vole species with multiple SRY copies in males and females

AUTHOR(S)
Fernández, Rosa; Barragán, María José L; Bullejos, Mónica; Marchal, Juan Alberto; Martínez, Sergio; de la Guardia, Rafael Díaz; Sánchez, Antonio
PUB. DATE
June 2002
SOURCE
Genome;Jun2002, Vol. 45 Issue 3, p600
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The SRY gene is a single-copy, male-specific gene, located on the Y chromosome in most mammals. However, recently we have described the presence of multiple polymorphic copies of this gene in both males and females of the vole species Microtus cabrerae. Here, we present the chromosomal localization of SRY gene copies in this species by fluorescent in situ hybridization (FISH). This technique localized these gene copies in the short arm, and hence in the euchromatic region, of the Y chromosome. Furthermore, several copies of the SRY gene are located on the X chromosome. These copies are spread along the entire heterochromatic region of the X chromosome, occupying the whole short arm, the centromeric region, and the pericentromeric region of the long arm.Key words: FISH mapping, Micotus cabrerae, SRY gene, X chromosome, Y chromosome.Chez la plupart des mammifères, le gène SRY est un gène à copie unique, présent exclusivement chez les mâles et situé sur le chromosome Y. Cependant, les auteurs ont récemment décrit la présence de copies multiples et polymorphes de ce gène chez les mâles et les femelles du campagnol de Cabrera, Microtus cabrerae. Les auteurs ont réalisé la localisation chromosomique des copies du gène SRY chez cette espèce par hybridation in situ en fluorescence (FISH). Cette technique a permis de localiser ces copies sur le bras court du chromosome Y et donc au sein d'une région euchromatique de ce chromosome. De plus, plusieurs copies du gène SRY sont situées sur le chromosome X. Ces copies sont dispersées tout au long de la région hétérochromatique du chromosome X, laquelle occupe la totalité du bras court ainsi que les régions centromérique et péricentromérique du bras long.Mots clés : cartographie FISH, Microtus cabrerae, gène SRY, chromosome X, chromosome Y.[Traduit par la Rédaction]
ACCESSION #
10579483

 

Related Articles

  • Meiotic studies in an azoospermic boar carrying a Y;14 translocation. Pinton, A.; Letron, I. Raymond; Berland, H. M.; Bonnet, N.; Calgaro, A.; Garnier-Bonnet, A.; Yerle, M.; Ducos, A. // Cytogenetic & Genome Research;2008, Vol. 120 Issue 1/2, p106 

    A reciprocal translocation between the q arm of the Y chromosome and the q arm of chromosome 14 was identified in a young, phenotypically normal boar presenting azoospermia. Testicular biopsies were analyzed by classical histological and immunolocalization techniques, and by fluorescence in situ...

  • Assignment of the thymosin beta 4 X/Y chromosome (TMSB4X/Y) gene to tammar wallaby chromosome 5p by fluorescence in situ hybridisation. Waters, P. D.; Sankovic, N.; Kirby, P. J.; Delbridge, M. L.; Graves, J. A. // Cytogenetic & Genome Research;2003, Vol. 103 Issue 1/2, p203F 

    Reports the assignment of the thymosin beta 4 X/Y chromosome (TMSB4X/Y) gene to tammar wallaby chromosome 5p by fluorescence in situ hybridization. Comparison of gene content of human sex chromosomes with those of distantly related mammals; Details of materials and methods; Cloning and...

  • Organizational Variation of DYZ1 Repeat Sequences on the Human Y Chromosome and Its Diagnostic Potentials. Rahman, Mohammed Mahidur; Bashamboo, Anu; Prasad, Aparna; Pathak, Deepali; Ali, Sher // DNA & Cell Biology;Sep2004, Vol. 23 Issue 9, p561 

    The long arm of the human Y chromosome is flecked with various fractions of repetitive DNA. DYZ1 is one such fraction, which is organized tandemly as an array of a 3.4-kb repeat ranging from 2000–4000 copies in normal males. We have studied the organizational variation of the DYZ1...

  • Different origins of ZZ/ZW sex chromosomes in closely related medaka fishes, Oryzias javanicus and O. hubbsi. Satoshi Hamaguchi; Mitsuru Sakaizumi // Chromosome Research;Aug2008, Vol. 16 Issue 5, p801 

    Abstract  Although the sex-determining gene DMY has been identified on the Y chromosome in the medaka, Oryzias latipes, this gene is absent in most Oryzias species. Recent comparative studies have demonstrated that, in the javanicus species group, Oryzias dancena and Oryzias minutillus...

  • A de-novo (Y;13) translocation in an oligozoospermic patient: cytogenetic and molecular studies and prognosis for PGD. Alves, C.; Carvalho, F.; Sousa, M.; Cremades, N.; Barros, A. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;2002 Supplement 2, Vol. 4 Issue S2, p45 

    Objective: Translocations between autosomes and the Y chromosome are seen in one in 2000 live-born children. The most common type of translocation involves an acrocentric chromosome and the heterochromatic portion of the Y chromosome. There are only two reports in the literature referring to...

  • Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat. Alexandrov, Oleg S.; Divashuk, Mikhail G.; Yakovin, Nikolay A.; Karlov, Gennady I. // Comparative Cytogenetics;2012, Vol. 6 Issue 3, p239 

    Humulus japonicus Siebold & Zucc (Japanese hop) is a dioecious species of the family Cannabaceae. he chromosome number is 2n = 16 = 14 + XX for females and 2n = 17 = 14 + XY1Y2 for male. To date, no fluorescence in situ hybridization (FISH) markers have been established for the identification of...

  • Complex mosaicism in sex reversed SRY+ male twins. DesGroseilliers, M.; Fortin, F.; Lemyre, E.; Lemieux, N. // Cytogenetic & Genome Research;2006, Vol. 112 Issue 1/2, p176 

    Sex reversal is characterized by discordance between genetic and phenotypic sex. Most XX males result from an unequal interchange between X and Y chromosomes during paternal meiosis, therefore transferring SRY to the X chromosome, which explains the male development in the presence of an...

  • A family case of fertile human 45,X,psu dic(15;Y) males. Wimmer, R.; Schempp, W.; Gopinath, P. M.; Nagarajappa, C. S.; Chandra, N.; Palaniappan, I.; Hansmann, I. // Cytogenetic & Genome Research;2006, Vol. 115 Issue 1, p94 

    We report on a familial case including four male probands from three generations with a 45,X,psu dic(15;Y)(p11.2;q12) karyotype. 45,X is usually associated with a female phenotype and only rarely with maleness, due to translocation of small Y chromosomal fragments to autosomes. These male...

  • Does differential selection on the 5S rDNA explain why the rainbow trout sex chromosome heteromorphism is not linked to the SEX locus? Phillips, R. B.; Noakes, M. A.; Morasch, M.; Felip, A.; Thorgaard, G. H. // Cytogenetic & Genome Research;2004, Vol. 105 Issue 1, p122 

    Many but not all rainbow trout strains have morphologically distinguishable sex chromosomes. In these strains, the short arm of the X has multiple copies of 5S rDNA and a bright DAPI band near the centromere, both of which are missing from the Y chromosome, which has a very small short arm. We...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics