Mapping the SRY gene in Microtus cabrerae: a vole species with multiple SRY copies in males and females

Fernández, Rosa; Barragán, María José L; Bullejos, Mónica; Marchal, Juan Alberto; Martínez, Sergio; de la Guardia, Rafael Díaz; Sánchez, Antonio
June 2002
Genome;Jun2002, Vol. 45 Issue 3, p600
Academic Journal
The SRY gene is a single-copy, male-specific gene, located on the Y chromosome in most mammals. However, recently we have described the presence of multiple polymorphic copies of this gene in both males and females of the vole species Microtus cabrerae. Here, we present the chromosomal localization of SRY gene copies in this species by fluorescent in situ hybridization (FISH). This technique localized these gene copies in the short arm, and hence in the euchromatic region, of the Y chromosome. Furthermore, several copies of the SRY gene are located on the X chromosome. These copies are spread along the entire heterochromatic region of the X chromosome, occupying the whole short arm, the centromeric region, and the pericentromeric region of the long arm.Key words: FISH mapping, Micotus cabrerae, SRY gene, X chromosome, Y chromosome.Chez la plupart des mammifères, le gène SRY est un gène à copie unique, présent exclusivement chez les mâles et situé sur le chromosome Y. Cependant, les auteurs ont récemment décrit la présence de copies multiples et polymorphes de ce gène chez les mâles et les femelles du campagnol de Cabrera, Microtus cabrerae. Les auteurs ont réalisé la localisation chromosomique des copies du gène SRY chez cette espèce par hybridation in situ en fluorescence (FISH). Cette technique a permis de localiser ces copies sur le bras court du chromosome Y et donc au sein d'une région euchromatique de ce chromosome. De plus, plusieurs copies du gène SRY sont situées sur le chromosome X. Ces copies sont dispersées tout au long de la région hétérochromatique du chromosome X, laquelle occupe la totalité du bras court ainsi que les régions centromérique et péricentromérique du bras long.Mots clés : cartographie FISH, Microtus cabrerae, gène SRY, chromosome X, chromosome Y.[Traduit par la Rédaction]


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