Incorporation of [sup 13] C glucose into nicotinamide in E. coli and in S. cerevisiae

Gupta, Ram Nath; Hemscheidt, Thomas; Sayer, Brian G; Spenser, Ian D
April 2001
Canadian Journal of Chemistry;Apr2001, Vol. 79 Issue 4, p418
Academic Journal
The mode of incorporation into nicotinamide of label from [sup 13] C-labeled samples of D-glucose, in Escherichia coli and Saccharomyces cerevisiae, was determined by means of [sup 13] C NMR spectroscopy. The results, which confirm and extend early studies with radioactive tracers, permit a definitive choice to be made between alternative biogenetic proposals.Key words: nicotinic acid, nicotinamide, biosynthesis, glucose incorporation, [sup 13] C NMR.Faisant appel � la spectroscopie RMN du [sup 13] C, on a d�termin� le mode d'incorporation du [sup 13] C d'�chantillons de D-glucose marqu� dans le nicotinamide d'Escherichia coli et de Saccharomyces cerevisiae. Les r�sultats, qui confirment et qui �tendent la port�e d'�tudes ant�rieures � l'aide de marqueurs radioactifs, permettent de faire un choix d�finitif entre les diverses propositions biog�n�tiques.Mots cl�s : acide nicotinique, nicotinamide, biosynth�se, incorporation du glucose, RMN du [sup 13] C.[Traduit par la R�daction]


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